CDC5L (NM_001253) Human Untagged Clone

CAT#: SC322498

CDC5L (untagged)-Human CDC5 cell division cycle 5-like (S. pombe) (CDC5L)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC5L
• Synonyms: CDC5; CDC5-LIKE; CEF1; dJ319D22.1; PCDC5RP
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322498 GCTTGGAGGAAGTGGCGGCTTTGAGTCCGGTGGCCCAATCGCTGTTACTACTTCTCTGAA
  GCTCCTCTCGGCTGCTTGCCGAGACACCCTGCCGCCAAGATGCCTCGAATTATGATCAAG
  GGGGGCGTATGGAGGAATACCGAGGATGAAATTCTGAAAGCAGCGGTAATGAAATATGGG
  AAAAATCAGTGGTCTAGGATTGCCTCATTGCTGCATAGAAAATCAGCAAAGCAGTGCAAA
  GCCAGATGGTATGAATGGCTGGATCCAAGCATTAAGAAGACAGAATGGTCCAGAGAAGAA
  GAGGAAAAACTCTTGCACTTGGCCAAGTTGATGCCAACTCAGTGGAGGACCATTGCTCCA
  ATCATTGGAAGAACAGCGGCCCAGTGCTTAGAACACTATGAATTTCTTCTGGATAAAGCT
  GCCCAAAGAGACAATGAAGAGGAAACAACAGATGATCCACGAAAACTTAAACCTGGAGAA
  ATAGATCCAAATCCAGAAACAAAACCAGCGCGGCCTGATCCAATTGATATGGATGAGGAT
  GAACTTGAGATGCTTTCTGAAGCCAGAGCCCGCTTGGCTAATACTCAGGGAAAGAAGGCC
  AAGAGGAAAGCAAGAGAGAAACAATTGGAAGAAGCAAGACGTCTTGCTGCCCTCCAAAAA
  AGAAGAGAACTTCGAGCAGCTGGCATAGAAATTCAGAAGAAAAGAAAAAGGAAGAGAGGA
  GTTGATTATAATGCCGAAATCCCATTTGAAAAAAAGCCTGCCCTTGGTTTTTATGATACT
  TCTGAGGAAAACTACCAAGCTCTTGACGCAGATTTCAGGAAATTAAGACAACAGGATCTT
  GATGGGGAGCTAAGATCTGAAAAAGAAGGAAGAGATAGAAAAAAAGACAAACAGCATTTG
  AAAAGGAAAAAAGAATCTGATTTACCATCAGCTATTCTTCAAACTAGTGGTGTTTCTGAA
  TTTACTAAAAAGAGAAGCAAACTAGTACTTCCTGCCCCTCAGATTTCAGATGCAGAACTC
  CAGGAAGTTGTAAAAGTAGGCCAAGCGAGTGAAATTGCACGTCAAACTGCCGAGGAATCT
  GGCATAACAAATTCTGCTTCCAGTACACTTTTGTCTGAGTACAATGTCACCAACAACAGC
  GTTGCTCTTAGAACACCACGAACACCAGCTTCCCAGGACAGAATTCTGCAGGAAGCCCAG
  AACCTCATGGCCCTCACCAATGTGGACACCCCATTGAAAGGTGGACTTAATACCCCATTG
  CATGAGAGTGACTTCTCAGGTGTAACTCCACAGCGACAAGTTGTACAGACTCCAAACACA
  GTTCTCTCTACTCCATTCAGGACTCCTTCTAATGGAGCTGAAGGGCTGACTCCCCGGAGT
  GGAACAACTCCCAAACCAGTTATTAACTCTACTCCGGGTAGAACTCCTCTTCGAGACAAG
  TTAAACATTAATCCCGAGGATGGAATGGCAGACTATAGTGATCCCTCTTACGTGAAGCAG
  ATGGAAAGAGAATCCCGAGAACATCTCCGTTTAGGGTTGTTGGGCCTTCCTGCCCCTAAG
  AATGATTTTGAAATTGTTCTACCAGAAAATGCCGAGAAGGAGCTGGAAGAACGTGAAATA
  GATGATACTTACATTGAAGATGCTGCTGATGTGGATGCTCGAAAGCAGGCCATACGAGAT
  GCAGAGCGTGTAAAGGAAATGAAACGAATGCATAAAGCTGTCCAGAAAGATCTGCCAAGA
  CCATCAGAAGTAAATGAAACTATTCTAAGACCCTTAAATGTAGAACCGCCTTTAACAGAT
  TTACAGAAAAGTGAAGAACTAATCAAAAAAGAAATGATCACAATGCTTCATTATGACCTT
  CTACATCACCCTTATGAACCATCTGGAAATAAAAAAGGCAAAACTGTAGGGTTTGGTACC
  AATAATTCAGAGCACATTACCTATCTGGAACATAATCCTTATGAAAAGTTCTCCAAAGAA
  GAGCTGAAAAAGGCCCAGGATGTTTTGGTGCAGGAGATGGAAGTGGTTAAACAAGGAATG
  AGCCATGGAGAGCTCTCAAGTGAAGCTTATAACCAGGTGTGGGAAGAATGCTACAGTCAA
  GTTTTATATCTTCCTGGGCAGAGCCGCTACACACGGGCCAATCTGGCTAGTAAAAAGGAC
  AGAATTGAATCACTTGAAAAGAGGCTCGAGATAAACAGGGGTCACATGACGACAGAAGCC
  AAGAGGGCTGCAAAGATGGAAAAGAAGATGAAAATTTTGCTTGGGGGTTACCAGTCTCGT
  GCTATGGGGCTCATGAAACAGTTGAATGACTTATGGGACCAAATTGAACAGGCTCACTTG
  GAGTTACGCACTTTTGAAGAACTCAAGAAACATGAAGATTCTGCTATTCCCCGGAGGCTA
  GAGTGTCTAAAAGAAGACGTTCAGCGACAACAAGAAAGAGAAAAGGAACTTCAACATAGA
  TATGCTGATTTGCTGCTGGAGAAAGAGACTTTAAAGTCAAAATTCTGAAGTACAGTTTAT
  ATTCTGTCACAGGATTAATTAATTGCCGGTTTTCATACTCTAGAAGGCTGAAACTGATGT
  TTATCTTCATTGACAAATTTACCCACCATCTGTGGTTTTTCAGTTGTTTATTTTAAATGA
  TATCGATCTTACACATTCTGTGTATAAAGACCTTAACTCCACAGGACGGACATTTTAGAG
  TTTAAATTATTAAGGCTATCATTCTTTTAGTAATGTCATATTTGCAAACTTTTTTAGTTT
  TGGCCTTTAATTTAAAAAGCCTAATTTTAAAGTGCTGCCTGTGAGTAACTCTTGAATAAA
  AACAAAATATAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001253
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253.2, NP_001244.1
• RefSeq Size: 3012 bp
• RefSeq ORF: 2409 bp
• Locus ID: 988
• Cytogenetics: 6p21.1
• Domains: myb_DNA-binding
• Protein Families: Transcription Factors
• Protein Pathways: Spliceosome
• Gene Summary: 'The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [provided by RefSeq, Jul 2008]'