CDC5L (NM_001253) Human Tagged ORF Clone

CAT#: RG201702

CDC5L (GFP-tagged) - Human CDC5 cell division cycle 5-like (S. pombe) (CDC5L)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CDC5L
• Synonyms: CDC5; CDC5-LIKE; CEF1; dJ319D22.1; PCDC5RP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201702 representing NM_001253
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCGAATTATGATCAAGGGGGGCGTATGGAGGAATACCGAGGATGAAATTCTGAAAGCAGCGGTAA
  TGAAATATGGGAAAAATCAGTGGTCTAGGATTGCCTCATTGCTGCATAGAAAATCAGCAAAGCAGTGCAA
  AGCCAGATGGTATGAATGGCTGGATCCAAGCATTAAGAAGACAGAATGGTCCAGAGAAGAAGAGGAAAAA
  CTCTTGCACTTGGCCAAGTTGATGCCAACTCAGTGGAGGACCATTGCTCCAATCATTGGAAGAACAGCGG
  CCCAGTGCTTAGAACACTATGAATTTCTTCTGGATAAAGCTGCCCAAAGAGACAATGAAGAGGAAACAAC
  AGATGATCCACGAAAACTTAAACCTGGAGAAATAGATCCAAATCCAGAAACAAAACCAGCGCGGCCTGAT
  CCAATTGATATGGATGAGGATGAACTTGAGATGCTTTCTGAAGCCAGAGCCCGCTTGGCTAATACTCAGG
  GAAAGAAGGCCAAGAGGAAAGCAAGAGAGAAACAATTGGAAGAAGCAAGACGTCTTGCTGCCCTCCAAAA
  AAGAAGAGAACTTCGAGCAGCTGGCATAGAAATTCAGAAGAAAAGAAAAAGGAAGAGAGGAGTTGATTAT
  AATGCCGAAATCCCATTTGAAAAAAAGCCTGCCCTTGGTTTTTATGATACTTCTGAGGAAAACTACCAAG
  CTCTTGACGCAGATTTCAGGAAATTAAGACAACAGGATCTTGATGGGGAGCTAAGATCTGAAAAAGAAGG
  AAGAGATAGAAAAAAAGACAAACAGCATTTGAAAAGGAAAAAAGAATCTGATTTACCATCAGCTATTCTT
  CAAACTAGTGGTGTTTCTGAATTTACTAAAAAGAGAAGCAAACTAGTACTTCCTGCCCCTCAGATTTCAG
  ATGCAGAACTCCAGGAAGTTGTAAAAGTAGGCCAAGCGAGTGAAATTGCACGTCAAACTGCCGAGGAATC
  TGGCATAACAAATTCTGCTTCCAGTACACTTTTGTCTGAGTACAATGTCACCAACAACAGCGTTGCTCTT
  AGAACACCACGAACACCAGCTTCCCAGGACAGAATTCTGCAGGAAGCCCAGAACCTCATGGCCCTCACCA
  ATGTGGACACCCCATTGAAAGGTGGACTTAATACCCCATTGCATGAGAGTGACTTCTCAGGTGTAACTCC
  ACAGCGACAAGTTGTACAGACTCCAAACACAGTTCTCTCTACTCCATTCAGGACTCCTTCTAATGGAGCT
  GAAGGGCTGACTCCCCGGAGTGGAACAACTCCCAAACCAGTTATTAACTCTACTCCGGGTAGAACTCCTC
  TTCGAGACAAGTTAAACATTAATCCCGAGGATGGAATGGCAGACTATAGTGATCCCTCTTACGTGAAGCA
  GATGGAAAGAGAATCCCGAGAACATCTCCGTTTAGGGTTGTTGGGCCTTCCTGCCCCTAAGAATGATTTT
  GAAATTGTTCTACCAGAAAATGCCGAGAAGGAGCTGGAAGAACGTGAAATAGATGATACTTACATTGAAG
  ATGCTGCTGATGTGGATGCTCGAAAGCAGGCCATACGAGATGCAGAGCGTGTAAAGGAAATGAAACGAAT
  GCATAAAGCTGTCCAGAAAGATCTGCCAAGACCATCAGAAGTAAATGAAACTATTCTAAGACCCTTAAAT
  GTAGAACCGCCTTTAACAGATTTACAGAAAAGTGAAGAACTAATCAAAAAAGAAATGATCACAATGCTTC
  ATTATGACCTTCTACATCACCCTTATGAACCATCTGGAAATAAAAAAGGCAAAACTGTAGGGTTTGGTAC
  CAATAATTCAGAGCACATTACCTATCTGGAACATAATCCTTATGAAAAGTTCTCCAAAGAAGAGCTGAAA
  AAGGCCCAGGATGTTTTGGTGCAGGAGATGGAAGTGGTTAAACAAGGAATGAGCCATGGAGAGCTCTCAA
  GTGAAGCTTATAACCAGGTGTGGGAAGAATGCTACAGTCAAGTTTTATATCTTCCTGGGCAGAGCCGCTA
  CACACGGGCCAATCTGGCTAGTAAAAAGGACAGAATTGAATCACTTGAAAAGAGGCTCGAGATAAACAGG
  GGTCACATGACGACAGAAGCCAAGAGGGCTGCAAAGATGGAAAAGAAGATGAAAATTTTGCTTGGGGGTT
  ACCAGTCTCGTGCTATGGGGCTCATGAAACAGTTGAATGACTTATGGGACCAAATTGAACAGGCTCACTT
  GGAGTTACGCACTTTTGAAGAACTCAAGAAACATGAAGATTCTGCTATTCCCCGGAGGCTAGAGTGTCTA
  AAAGAAGACGTTCAGCGACAACAAGAAAGAGAAAAGGAACTTCAACATAGATATGCTGATTTGCTGCTGG
  AGAAAGAGACTTTAAAGTCAAAATTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201702 representing NM_001253
  Red=Cloning site Green=Tags(s)
  
  MPRIMIKGGVWRNTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARWYEWLDPSIKKTEWSREEEEK
  LLHLAKLMPTQWRTIAPIIGRTAAQCLEHYEFLLDKAAQRDNEEETTDDPRKLKPGEIDPNPETKPARPD
  PIDMDEDELEMLSEARARLANTQGKKAKRKAREKQLEEARRLAALQKRRELRAAGIEIQKKRKRKRGVDY
  NAEIPFEKKPALGFYDTSEENYQALDADFRKLRQQDLDGELRSEKEGRDRKKDKQHLKRKKESDLPSAIL
  QTSGVSEFTKKRSKLVLPAPQISDAELQEVVKVGQASEIARQTAEESGITNSASSTLLSEYNVTNNSVAL
  RTPRTPASQDRILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQVVQTPNTVLSTPFRTPSNGA
  EGLTPRSGTTPKPVINSTPGRTPLRDKLNINPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDF
  EIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSEVNETILRPLN
  VEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHITYLEHNPYEKFSKEELK
  KAQDVLVQEMEVVKQGMSHGELSSEAYNQVWEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINR
  GHMTTEAKRAAKMEKKMKILLGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECL
  KEDVQRQQEREKELQHRYADLLLEKETLKSKF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001253
• ORF Size: 2406 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253.2, NP_001244.1
• RefSeq Size: 3012 bp
• RefSeq ORF: 2409 bp
• Locus ID: 988
• Cytogenetics: 6p21.1
• Domains: myb_DNA-binding
• Protein Families: Transcription Factors
• Protein Pathways: Spliceosome
• Gene Summary: 'The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [provided by RefSeq, Jul 2008]'