CDC5L (NM_001253) Human Untagged Clone

CAT#: SC119335

CDC5L (untagged)-Human CDC5 cell division cycle 5-like (S. pombe) (CDC5L)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC5L
• Synonyms: CDC5; CDC5-LIKE; CEF1; dJ319D22.1; PCDC5RP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119335 sequence for NM_001253 edited (data generated by NextGen Sequencing)
  ATGCCTCGAATTATGATCAAGGGGGGCGTATGGAGGAATACCGAGGATGAAATTCTGAAA
  GCAGCGGTAATGAAATATGGGAAAAATCAGTGGTCTAGGATTGCCTCATTGCTGCATAGA
  AAATCAGCAAAGCAGTGCAAAGCCAGATGGTATGAATGGCTGGATCCAAGCATTAAGAAG
  ACAGAATGGTCCAGAGAAGAAGAGGAAAAACTCTTGCACTTGGCCAAGTTGATGCCAACT
  CAGTGGAGGACCATTGCTCCAATCATTGGAAGAACAGCGGCCCAGTGCTTAGAACACTAT
  GAATTTCTTCTGGATAAAGCTGCCCAAAGAGACAATGAAGAGGAAACAACAGATGATCCA
  CGAAAACTTAAACCTGGAGAAATAGATCCAAATCCAGAAACAAAACCAGCGCGGCCTGAT
  CCAATTGATATGGATGAGGATGAACTTGAGATGCTTTCTGAAGCCAGAGCCCGCTTGGCT
  AATACTCAGGGAAAGAAGGCCAAGAGGAAAGCAAGAGAGAAACAATTGGAAGAAGCAAGA
  CGTCTTGCTGCCCTCCAAAAAAGAAGAGAACTTCGAGCAGCTGGCATAGAAATTCAGAAG
  AAAAGAAAAAGGAAGAGAGGAGTTGATTATAATGCCGAAATCCCATTTGAAAAAAAGCCT
  GCCCTTGGTTTTTATGATACTTCTGAGGAAAACTACCAAGCTCTTGACGCAGATTTCAGG
  AAATTAAGACAACAGGATCTTGATGGGGAGCTAAGATCTGAAAAAGAAGGAAGAGATAGA
  AAAAAAGACAAACAGCATTTGAAAAGGAAAAAAGAATCTGATTTACCATCAGCTATTCTT
  CAAACTAGTGGTGTTTCTGAATTTACTAAAAAGAGAAGCAAACTAGTACTTCCTGCCCCT
  CAGATTTCAGATGCAGAACTCCAGGAAGTTGTAAAAGTAGGCCAAGCGAGTGAAATTGCA
  CGTCAAACTGCCGAGGAATCTGGCATAACAAATTCTGCTTCCAGTACACTTTTGTCTGAG
  TACAATGTCACCAACAACAGCGTTGCTCTTAGAACACCACGAACACCAGCTTCCCAGGAC
  AGAATTCTGCAGGAAGCCCAGAACCTCATGGCCCTCACCAATGTGGACACCCCATTGAAA
  GGTGGACTTAATACCCCATTGCATGAGAGTGACTTCTCAGGTGTAACTCCACAGCGACAA
  GTTGTACAGACTCCAAACACAGTTCTCTCTACTCCATTCAGGACTCCTTCTAATGGAGCT
  GAAGGGCTGACTCCCCGGAGTGGAACAACTCCCAAACCAGTTATTAACTCTACTCCGGGT
  AGAACTCCTCTTCGAGACAAGTTAAACATTAATCCCGAGGATGGAATGGCAGACTATAGT
  GATCCCTCTTACGTGAAGCAGATGGAAAGAGAATCCCGAGAACATCTCCGTTTAGGGTTG
  TTGGGCCTTCCTGCCCCTAAGAATGATTTTGAAATTGTTCTACCAGAAAATGCCGAGAAG
  GAGCTGGAAGAACGTGAAATAGATGATACTTACATTGAAGATGCTGCTGATGTGGATGCT
  CGAAAGCAGGCCATACGAGATGCAGAGCGTGTAAAGGAAATGAAACGAATGCATAAAGCT
  GTCCAGAAAGATCTGCCAAGACCATCAGAAGTAAATGAAACTATTCTAAGACCCTTAAAT
  GTAGAACCGCCTTTAACAGATTTACAGAAAAGTGAAGAACTAATCAAAAAAGAAATGATC
  ACAATGCTTCATTATGACCTTCTACATCACCCTTATGAACCATCTGGAAATAAAAAAGGC
  AAAACTGTAGGGTTTGGTACCAATAATTCAGAGCACATTACCTATCTGGAACATAATCCT
  TATGAAAAGTTCTCCAAAGAAGAGCTGAAAAAGGCCCAGGATGTTTTGGTGCAGGAGATG
  GAAGTGGTTAAACAAGGAATGAGCCATGGAGAGCTCTCAAGTGAAGCTTATAACCAGGTG
  TGGGAAGAATGCTACAGTCAAGTTTTATATCTTCCTGGGCAGAGCCGCTACACACGGGCC
  AATCTGGCTAGTAAAAAGGACAGAATTGAATCACTTGAAAAGAGGCTCGAGATAAACAGG
  GGTCACATGACGACAGAAGCCAAGAGGGCTGCAAAGATGGAAAAGAAGATGAAAATTTTG
  CTTGGGGGTTACCAGTCTCGTGCTATGGGGCTCATGAAACAGTTGAATGACTTATGGGAC
  CAAATTGAACAGGCTCACTTGGAGTTACGCACTTTTGAAGAACTCAAGAAACATGAAGAT
  TCTGCTATTCCCCGGAGGCTAGAGTGTCTAAAAGAAGACGTTCAGCGACAACAAGAAAGA
  GAAAAGGAACTTCAACATAGATATGCTGATTTGCTGCTGGAGAAAGAGACTTTAAAGTCA
  AAATTCTGA
  
  Clone variation with respect to NM_001253.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001253 unedited
  TTGTAACACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTGGAGGAAGTGGC
  GGCTTTGAGTCCGGTGGCCCAATCGCTGTTACTACTTCTCTGAAGCTCCTCTCGGCTGCT
  TGCCGAGACACCCTGCCGCCAAGATGCCTCGAATTATGATCAAGGGGGGCGTATGGAGGA
  ATACCGAGGATGAAATTCTGAAAGCAGCGGTAATGAAATATGGGAAAAATCAGTGGTCTA
  GGATTGCCTCATTGCTGCATAGAAAATCAGCAAAGCAGTGCAAAGCCAGATGGTATGAAT
  GGCTGGATCCAAGCATTAAGAAGACAGAATGGTCCAGAGAAGAAGAGGAAAAACTCTTGC
  ACTTGGCCAAGTTGATGCCAACTCAGTGGAGGACCATTGCTCCAATCATTGGAAGAACAG
  CGGCCCAGTGCTTAGAACACTATGAATTTCTTCTGGATAAAGCTGCCCAAAGAGACAATG
  AAGAGGAAACAACAGATGATCCACGAAAACTTAAACCTGGAGAAATAGATCCAAATCCAG
  AAACAAAACCAGCGCGGCCTGATCCAATTGATATGGATGAGGATGAACTTGAGATGCTTT
  CTGAAGCCAGAGCCCGCTTGGCTAATACTCAGGGAAAGAAGGCCAAGAGGAAAGCAAGAG
  AGATACAATTGGAAGAAGCAAGACGTCTTGCTGCCCTCCAAAAAAGAAGAGAACTTCGAG
  CAGCTGGCATAGAAATTCAGAAGAAAAGAANAAGGAAGAGAGGAGTTGATTTATATGCCG
  AAATCCATTTGAAAAAAGCCTGCCCTTGGGTTTTATGATACTTCTGAGGAAACTACCAGC
  TCTTGACGCAGATTCAGGAAAATAGACACAGATCTTGATGGGGAGCTAGACTGAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001253 unedited
  TGACCGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTATATT
  TTGGTTTTATTCAAGAGTTACTCACAGGCAGCACTTTAAAATTAGGCTTTTTAAATTAAA
  GGCCAAAACTAAAAAAGTTTGCAAATATGACATTACTAAAAGAATGATAGCCTTAATAAT
  TTAAACTCTAAAATGTCCGTCCTGTGGAGTTAAGGTCTTTATACACAGAATGTGTAAGAT
  CGATATCATTTAAAATAAACAACTGAAAAACCACAGATGGGGGGTAAATTTGTCAATGAA
  GATAAACATCAGTTTCAGCCTTCTAAAGTATGAAAACCGGCAATTAATTAATCCTGTGAC
  AGAATATAAACTGTACTTCAAAATTTTGACTTTAAAGTCTCTTTCTCCAGCAGCAAATCA
  GCATATCTATGTTGAAGTTCCTTTTCTCTTTCTTGTTGGCGCTGAACGTCTTCTTTTAGA
  CACTCTAGCCTCCGGGGAATAGCAGAATCTTCATGTTTCTTGAGTTCTTCAAAAGTGCGT
  AACTCCAAGTGAGCCTGTTCAATTTGGTCCCATAAGTCATTCAACTGTTTCATGAGCCCC
  ATAGCACGAGACTGGTAACCCCCAAGCAAAATTTTCATCTTCTTTTCCATCTTTGCAGCC
  CTCTTGGCTTCTGTCGTCATGTGACCCCTGTTTATCTCGAGCCTCTTTTCAAGTGATTCA
  ATTCTGTCCTTTTTACTAAGCAGATTGGCCCGTGTGTAGCGGCTCTGCCCACGAAGATAA
  TAAACTTGACTGTAACATTCTTCCCACACCTGGTTATAGCTTCACTTGAAAGACTCTCAT
  GGCTCATTCCTTGGTTTAACACTTCCATCTCCTGCACCAANCATTCCTGGGCCTTTTTCA
  ACTCTTTCTTGGAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001253
• Insert Size: 2940 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253.2, NP_001244.1
• RefSeq Size: 3012 bp
• RefSeq ORF: 2409 bp
• Locus ID: 988
• Cytogenetics: 6p21.1
• Domains: myb_DNA-binding
• Protein Families: Transcription Factors
• Protein Pathways: Spliceosome
• Gene Summary: 'The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [provided by RefSeq, Jul 2008]'