VPS33A (NM_022916) Human Tagged ORF Clone

CAT#: RC202151

VPS33A (Myc-DDK-tagged)-Human vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A)

Product Images

Western blot validation of overexpression lysate (Cat# LY411454) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202151 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified VPS33A protein (Cat# TP302151). The protein was produced from HEK293T cells transfected with VPS33A cDNA clone (Cat# RC202151) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VPS33A
• Synonyms: MPSPS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202151 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTCATCTGTCCTACGGCCGAGTGAACCTAAACGTGTTGCGCGAGGCGGTGCGTCGCGAGCTGC
  GCGAGTTCCTGGACAAGTGCGCAGGAAGCAAGGCAATAGTTTGGGATGAATACCTAACTGGACCCTTTGG
  CCTGATTGCACAGTATTCACTATTGAAGGAACATGAAGTGGAAAAAATGTTCACACTTAAAGGAAATCGT
  TTGCCGGCAGCTGATGTGAAGAATATAATTTTTTTTGTCAGACCCAGGCTAGAGTTGATGGATATAATCG
  CTGAAAACGTGCTCAGTGAAGATAGACGAGGCCCAACGAGAGATTTTCATATTCTGTTTGTGCCACGCCG
  TAGCCTGTTGTGCGAACAGCGGTTGAAGGATCTGGGTGTCTTGGGATCCTTTATTCACAGGGAGGAGTAC
  AGCTTAGATCTCATTCCATTCGATGGGGATCTCTTATCCATGGAATCAGAGGGTGCATTCAAAGAGTGCT
  ACCTGGAGGGTGACCAGACGAGCCTGTACCACGCAGCCAAGGGGCTGATGACCCTGCAAGCTCTGTATGG
  AACGATCCCCCAGATCTTTGGGAAAGGAGAATGCGCTCGGCAAGTGGCCAATATGATGATCAGGATGAAG
  AGAGAGTTTACAGGAAGCCAGAATTCAATATTTCCTGTTTTTGATAATCTCTTGTTGCTTGATCGGAATG
  TGGATTTATTAACACCTCTTGCCACTCAGCTGACATATGAAGGACTCATTGATGAAATTTATGGCATTCA
  GAACAGTTATGTGAAATTACCTCCAGAGAAATTTGCACCTAAGAAACAGGGCGATGGTGGTAAGGACCTC
  CCCACGGAAGCAAAGAAGCTGCAGCTGAATTCTGCAGAGGAGCTCTATGCTGAGATCCGAGATAAGAACT
  TCAACGCAGTTGGCTCTGTGCTCAGCAAGAAAGCAAAGATCATCTCTGCAGCATTCGAGGAAAGACACAA
  TGCTAAGACCGTGGGGGAGATCAAGCAGTTTGTTTCCCAGTTGCCCCACATGCAGGCAGCAAGGGGCTCG
  CTTGCAAACCATACCTCAATTGCAGAATTGATCAAAGATGTCACTACTTCTGAAGACTTTTTTGATAAAT
  TAACCGTGGAACAGGAGTTTATGTCTGGAATAGACACTGATAAGGTCAACAATTACATTGAGGATTGTAT
  CGCCCAAAAGCACTCGTTGATCAAGGTGTTAAGACTAGTTTGCCTCCAATCCGTGTGTAATAGTGGGCTC
  AAACAAAAAGTTTTGGATTATTACAAAAGAGAGATTCTCCAGACATACGGCTATGAGCACATATTGACCT
  TACACAACCTGGAGAAGGCCGGCCTGCTGAAACCGCAGACGGGGGGCAGAAACAATTACCCAACTATACG
  GAAAACATTACGCCTCTGGATGGATGATGTTAATGAGCAAAACCCCACGGACATATCGTATGTGTACAGT
  GGGTATGCCCCGCTCAGTGTGCGGCTGGCCCAGCTGCTTTCCCGGCCTGGCTGGCGGAGCATCGAGGAGG
  TCCTCCGCATCCTCCCAGGGCCCCACTTTGAGGAGCGGCAGCCACTGCCCACAGGACTGCAGAAGAAACG
  TCAACCGGGAGAAAACCGAGTGACTCTGATATTTTTCCTTGGGGGCGTAACCTTCGCTGAAATTGCTGCC
  CTGCGATTTCTCTCCCAGTTGGAAGATGGAGGTACAGAATATGTCATTGCCACCACTAAACTAATGAATG
  GAACCAGTTGGATAGAGGCTCTGATGGAAAAACCTTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202151 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAHLSYGRVNLNVLREAVRRELREFLDKCAGSKAIVWDEYLTGPFGLIAQYSLLKEHEVEKMFTLKGNR
  LPAADVKNIIFFVRPRLELMDIIAENVLSEDRRGPTRDFHILFVPRRSLLCEQRLKDLGVLGSFIHREEY
  SLDLIPFDGDLLSMESEGAFKECYLEGDQTSLYHAAKGLMTLQALYGTIPQIFGKGECARQVANMMIRMK
  REFTGSQNSIFPVFDNLLLLDRNVDLLTPLATQLTYEGLIDEIYGIQNSYVKLPPEKFAPKKQGDGGKDL
  PTEAKKLQLNSAEELYAEIRDKNFNAVGSVLSKKAKIISAAFEERHNAKTVGEIKQFVSQLPHMQAARGS
  LANHTSIAELIKDVTTSEDFFDKLTVEQEFMSGIDTDKVNNYIEDCIAQKHSLIKVLRLVCLQSVCNSGL
  KQKVLDYYKREILQTYGYEHILTLHNLEKAGLLKPQTGGRNNYPTIRKTLRLWMDDVNEQNPTDISYVYS
  GYAPLSVRLAQLLSRPGWRSIEEVLRILPGPHFEERQPLPTGLQKKRQPGENRVTLIFFLGGVTFAEIAA
  LRFLSQLEDGGTEYVIATTKLMNGTSWIEALMEKPF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022916
• ORF Size: 1788 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022916.2, NM_022916.3, NM_022916.4, NP_075067.2
• RefSeq Size: 2628 bp
• RefSeq ORF: 1791 bp
• Locus ID: 65082
• Domains: Sec1
• MW: 67.6 kDa
• Gene Summary: This gene encodes a tethering protein and a core subunit of the homotypic fusion and protein sorting (HOPS) complex. The HOPS complex and a second endosomal tethering complex called the class C core vacuole/endosome tethering (CORVET) complex, perform diverse functions in endocytosis including membrane tethering, RabGTPase interaction, activation and proofreading of synaptic-soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) assembly to drive membrane fusion, and endosome-to-cytoskeleton attachment. The HOPS complex controls endosome maturation as well as endosome traffic to the lysosome. This complex is essential for vacuolar fusion and is required for adaptor protein complex 3-dependent transport from the golgi to the vacuole. The encoded protein belongs to the Sec1/Munc18 (SM) family of SNARE-mediated membrane fusion regulators. Naturally occurring mutations in this gene are associated with a novel mucopolysaccharidosis-like disease. [provided by RefSeq, Apr 2017]