VPS33A (NM_022916) Human Untagged Clone

CAT#: SC127193

VPS33A (untagged)-Human vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VPS33A
• Synonyms: MPSPS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127193 sequence for NM_022916 edited (data generated by NextGen Sequencing)
  ATGGCGGCTCATCTGTCCTACGGCCGAGTGAACCTAAACGTGTTGCGCGAGGCGGTGCGT
  CGCGAGCTGCGCGAGTTCCTGGACAAGTGCGCAGGAAGCAAGGCAATAGTTTGGGATGAA
  TACCTAACTGGACCCTTTGGCCTGATTGCACAGTATTCACTATTGAAGGAACATGAAGTG
  GAAAAAATGTTCACACTTAAAGGAAATCGTTTGCCGGCAGCTGATGTGAAGAATATAATT
  TTTTTTGTCAGACCCAGGCTAGAGTTGATGGATATAATCGCTGAAAACGTGCTCAGTGAA
  GATAGACGAGGCCCAACGAGAGATTTTCATATTCTGTTTGTGCCACGCCGTAGCCTGTTG
  TGCGAACAGCGGTTGAAGGATCTGGGTGTCTTGGGATCCTTTATTCACAGGGAGGAGTAC
  AGCTTAGATCTCATTCCATTCGATGGGGATCTCTTATCCATGGAATCAGAGGGTGCATTC
  AAAGAGTGCTACCTGGAGGGTGACCAGACGAGCCTGTACCACGCAGCCAAGGGGCTGATG
  ACCCTGCAAGCTCTGTATGGAACGATCCCCCAGATCTTTGGGAAAGGAGAATGCGCTCGG
  CAAGTGGCCAATATGATGATCAGGATGAAGAGAGAGTTTACAGGAAGCCAGAATTCAATA
  TTTCCTGTTTTTGATAATCTCTTGTTGCTTGATCGGAATGTGGATTTATTAACACCTCTT
  GCCACTCAGCTGACATATGAAGGACTCATTGATGAAATTTATGGCATTCAGAACAGTTAT
  GTGAAATTACCTCCAGAGAAATTTGCACCTAAGAAACAGGGCGATGGTGGTAAGGACCTC
  CCCACGGAAGCAAAGAAGCTGCAGCTGAATTCTGCAGAGGAGCTCTATGCTGAGATCCGA
  GATAAGAACTTCAACGCAGTTGGCTCTGTGCTCAGCAAGAAAGCAAAGATCATCTCTGCA
  GCATTCGAGGAAAGACACAATGCTAAGACCGTGGGGGAGATCAAGCAGTTTGTTTCCCAG
  TTGCCCCACATGCAGGCAGCAAGGGGCTCGCTTGCAAACCATACCTCAATTGCAGAATTG
  ATCAAAGATGTCACTACTTCTGAAGACTTTTTTGATAAATTAACCGTGGAACAGGAGTTT
  ATGTCTGGAATAGACACTGATAAGGTCAACAATTACATTGAGGATTGTATCGCCCAAAAG
  CACTCGTTGATCAAGGTGTTAAGACTAGTTTGCCTCCAATCCGTGTGTAATAGTGGGCTC
  AAACAAAAAGTTTTGGATTATTACAAAAGAGAGATTCTCCAGACATACGGCTATGAGCAC
  ATATTGACCTTACACAACCTGGAGAAGGCCGGCCTGCTGAAACCGCAGACGGGGGGCAGA
  AACAATTACCCAACTATACGGAAAACATTACGCCTCTGGATGGATGATGTTAATGAGCAA
  AACCCCACGGACATATCGTATGTGTACAGTGGGTATGCCCCGCTCAGTGTGCGGCTGGCC
  CAGCTGCTTTCCCGGCCTGGCTGGCGGAGCATCGAGGAGGTCCTCCGCATCCTCCCAGGG
  CCCCACTTTGAGGAGCGGCAGCCACTGCCCACAGGACTGCAGAAGAAACGTCAACCGGGA
  GAAAACCGAGTGACTCTGATATTTTTCCTTGGGGGCGTAACCTTCGCTGAAATTGCTGCC
  CTGCGATTTCTCTCCCAGTTGGAAGATGGAGGTACAGAATATGTCATTGCCACCACTAAA
  CTAATGAATGGAACCAGTTGGATAGAGGCTCTGATGGAAAAACCTTTCTAG
  
  Clone variation with respect to NM_022916.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022916 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGGTGGTGGAGCAAGATGGC
  GGCTCATCTGTCCTACGGCCGAGTGAACCTAAACGTGTTGCGCGAGGCGGTGCGTCGCGA
  GCTGCGCGAGTTCCTGGACAAGTGCGCAGGAAGCAAGGCAATAGTTTGGGATGAATACCT
  AACTGGACCCTTTGGCCTGATTGCACAGTATTCACTATTGAAGGAACATGAAGTGGAAAA
  AATGTTCACACTTAAAGGAAATCGTTTGCCGGCAGCTGATGTGAAGAATATAATTTTTTT
  TGTCAGACCCAGGCTAGAGTTGATGGATATAATCGCTGAAAACGTGCTCAGTGAAGATAG
  ACGAGGCCCAACGAGAGATTTTCATATTCTGTTTGTGCCACGCCGTAGCCTGTTGTGCGA
  ACAGCGGTTGAAGGATCTGGGTGTCTTGGGATCCTTTATTCACAGGGAGGAGTACAGCTT
  AGATCTCATTCCATTCGATGGGGATCTCTTATCCATGGAATCAGAGGGTGCATTCAAAGA
  GTGCTACCTGGAGGGTGACCAGACGAGCCTGTACCACGCAGCCAAGGGGCTGATGACCCT
  GNCAGCTCTGTATGGAACGATCCCCCAGATCTTTGGGAAAGGAGAAATGCGCTCGGCAGT
  GGCCATATGATGATCAGGATGAAGAGAGAGTTACAGGAAGCCAGATTCATATTTCCTGTT
  TTTGATATCTCTTGTGGCTGTCCGAAGGTGATTATTAACACCTCTGCCCTCAGCTGACTA
  TGAAGACTCATGATGAAATTATGCATCANNAACAGTATGTGAATTACCTCAGAGNATTTG
  ACTAGAACGGGCGATGGGTGGAAGGCCTTCCACGAGCAAGAACTGACTGATTGCGAGACT
  CTTTTCTGATCGGTACTCTCCCACCTTTGGTCTGGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022916 unedited
  CGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTAAAAGGGAGAACTTTTTTTTTTTATTTTCAGTTTCA
  CCTCCTCAGTCTCAGCTTTTTTATTTTAAAAACTGGCCCCGTTTGTTTGGCTGAATGGAT
  TATTTACCCCCACCATTCTTAATGTTTTTCCAAAGGGTTTTAATGAAAACCCTGAGCTTA
  AAAAACTCAAAACCAGGGGTGGTTTTACTCAGGGGGAATTTCTTCTATTCTTCTTTCTTC
  AACAAATTAGGGGCCAGGCACGGGGGCTCAAGCCTGTAATCCCAACACTGGGGGAGGCCC
  AGGCAGGGGGATCACCTGAGGGCAGGAGTTTGAAACCACCCTGGCCAACATGGGGAAACA
  CCATGGGTACTAAAAATACAAAAATTACCCGGGGGGGGGGGGGCATGCCTGTANTCCCAG
  CTACTCAGGAGGGTGAGGCAGGAAAATTGCTTGAACCTAAGAGGGGGAGGTTTCAATGAG
  GTGAAACCGTGCCACTGCACTCTAATCTGGGTGACAAAGCAAGACTCTCGTCTCAAGGAA
  AAAAAAAAAGGGGATTCCAAAGAGACGGAAAAGCGGTAACAGTTGTTTATTTTAGAAGCT
  GCCCCCAATATATTCTGTATTCCATGTTCTTCTAGGGGGTTTCCTTCCTTAACAATTTTT
  AAAAGAGTAGTTTATTTGCAGACCCTGGTAAGTTTCTCTGACATCCAAAAAGTTTTTCAT
  TAAAACTTATCAACTGGTCCATTAATATTTAAGGGGGGAAGAAATTTTTGTCCCCCTTTT
  TCAACGGGAAAAAATGCGGGGCACATTTCAGGGAGGGTCCCCCCAGGGAAAATTAAAGCC
  CTGGTTTTTTCCGGGGAATTTTTTTGGAACCTGGGCAAGGCTCCGCTTCAAAGGGGGCCT
  GGAGGGGGGGAACCTTTAAGTTTCCCCCAACCGGAAAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022916
• ORF Size: 1791 bp
• Insert Size: 2730
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_022916.3, NP_075067.2
• RefSeq Size: 2577
• RefSeq ORF: 1791
• Locus ID: 65082
• Domains: Sec1
• Gene Summary: This gene encodes a tethering protein and a core subunit of the homotypic fusion and protein sorting (HOPS) complex. The HOPS complex and a second endosomal tethering complex called the class C core vacuole/endosome tethering (CORVET) complex, perform diverse functions in endocytosis including membrane tethering, RabGTPase interaction, activation and proofreading of synaptic-soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) assembly to drive membrane fusion, and endosome-to-cytoskeleton attachment. The HOPS complex controls endosome maturation as well as endosome traffic to the lysosome. This complex is essential for vacuolar fusion and is required for adaptor protein complex 3-dependent transport from the golgi to the vacuole. The encoded protein belongs to the Sec1/Munc18 (SM) family of SNARE-mediated membrane fusion regulators. Naturally occurring mutations in this gene are associated with a novel mucopolysaccharidosis-like disease. [provided by RefSeq, Apr 2017]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.