VPS33A (NM_022916) Human Untagged Clone

CAT#: SC324636

VPS33A (untagged)-Human vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VPS33A
• Synonyms: MPSPS
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_022916.4 GGCAGGGGGTGGTGGAGCAAGATGGCGGCTCATCTGTCCTACGGCCGAGTGAACCTAAAC
  GTGTTGCGCGAGGCGGTGCGTCGCGAGCTGCGCGAGTTCCTGGACAAGTGCGCAGGAAGC
  AAGGCAATAGTTTGGGATGAATACCTAACTGGACCCTTTGGCCTGATTGCACAGTATTCA
  CTATTGAAGGAACATGAAGTGGAAAAAATGTTCACACTTAAAGGAAATCGTTTGCCGGCA
  GCTGATGTGAAGAATATAATTTTTTTTGTCAGACCCAGGCTAGAGTTGATGGATATAATC
  GCTGAAAACGTGCTCAGTGAAGATAGACGAGGCCCAACGAGAGATTTTCATATTCTGTTT
  GTGCCACGCCGTAGCCTGTTGTGCGAACAGCGGTTGAAGGATCTGGGTGTCTTGGGATCC
  TTTATTCACAGGGAGGAGTACAGCTTAGATCTCATTCCATTCGATGGGGATCTCTTATCC
  ATGGAATCAGAGGGTGCATTCAAAGAGTGCTACCTGGAGGGTGACCAGACGAGCCTGTAC
  CACGCAGCCAAGGGGCTGATGACCCTGCAAGCTCTGTATGGAACGATCCCCCAGATCTTT
  GGGAAAGGAGAATGCGCTCGGCAAGTGGCCAATATGATGATCAGGATGAAGAGAGAGTTT
  ACAGGAAGCCAGAATTCAATATTTCCTGTTTTTGATAATCTCTTGTTGCTTGATCGGAAT
  GTGGATTTATTAACACCTCTTGCCACTCAGCTGACATATGAAGGACTCATTGATGAAATT
  TATGGCATTCAGAACAGTTATGTGAAATTACCTCCAGAGAAATTTGCACCTAAGAAACAG
  GGCGATGGTGGTAAGGACCTCCCCACGGAAGCAAAGAAGCTGCAGCTGAATTCTGCAGAG
  GAGCTCTATGCTGAGATCCGAGATAAGAACTTCAACGCAGTTGGCTCTGTGCTCAGCAAG
  AAAGCAAAGATCATCTCTGCAGCATTCGAGGAAAGACACAATGCTAAGACCGTGGGGGAG
  ATCAAGCAGTTTGTTTCCCAGTTGCCCCACATGCAGGCAGCAAGGGGCTCGCTTGCAAAC
  CATACCTCAATTGCAGAATTGATCAAAGATGTCACTACTTCTGAAGACTTTTTTGATAAA
  TTAACCGTGGAACAGGAGTTTATGTCTGGAATAGACACTGATAAGGTCAACAATTACATT
  GAGGATTGTATCGCCCAAAAGCACTCGTTGATCAAGGTGTTAAGACTAGTTTGCCTCCAA
  TCCGTGTGTAATAGTGGGCTCAAACAAAAAGTTTTGGATTATTACAAAAGAGAGATTCTC
  CAGACATACGGCTATGAGCACATATTGACCTTACACAACCTGGAGAAGGCCGGCCTGCTG
  AAACCGCAGACGGGGGGCAGAAACAATTACCCAACTATACGGAAAACATTACGCCTCTGG
  ATGGATGATGTTAATGAGCAAAACCCCACGGACATATCGTATGTGTACAGTGGGTATGCC
  CCGCTCAGTGTGCGGCTGGCCCAGCTGCTTTCCCGGCCTGGCTGGCGGAGCATCGAGGAG
  GTCCTCCGCATCCTCCCAGGGCCCCACTTTGAGGAGCGGCAGCCACTGCCCACAGGACTG
  CAGAAGAAACGTCAACCGGGAGAAAACCGAGTGACTCTGATATTTTTCCTTGGGGGCGTA
  ACCTTCGCTGAAATTGCTGCCCTGCGATTTCTCTCCCAGTTGGAAGATGGAGGTACAGAA
  TATGTCATTGCCACCACTAAACTAATGAATGGAACCAGTTGGATAGAGGCTCTGATGGAA
  AAACCTTTCTAGGATGTTCAGAGGAGACTTAACAAGTGTACTGCAGAATAAACTACCTCT
  TTGAAGAAATTGCTGAAAGGAAGTAAAACCCCATAGAAGAAACATGGGAATACAGAATAT
  ATTCTGGGGTCAGCTTCTTAAATTATACTACTGTTTACTGCTTTCTCCGTCTCTTTTGTA
  TTCCCTTTTTTTTTTTCCTTTGAGACGGAGTCTTGCTCTGTCACCCAGACTAGAGTGCAG
  TGGCACGGTCTCACCTCACTGCAACCTCCACCTCCTAGGTTCAAGCAATTCTCCTGCCTC
  AGCCTCCTGAGTAGCTGGGACTACAGGCATGCACCACCACACCCGGCTAATTTTTGTATT
  TTTAGTAGCCATGGTGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAGG
  TGATCCACCTGCCTCGGCCTCCCACAGTGCTGGGATTACAGGCCTGAGCCACCGTGCCTG
  GCCCCTAATCTGCTGAAGAAAGAAGAATAGAAGAAAATCAACCTGAGTAAAAGCAGCACT
  GGTTTTGAGTTTTCTAAGCTCAGGGTCTTCATTAGAGACCTCTGGAAATACATTAAGGAT
  GGTGGGGGTAGATAATCCATTCAGCCAGACAAACGGGGCCAGCTCTTAAAATAAGAAAGC
  TGAGACTGAGGAGGTGAAACTGAAAATAAAAAGAGAAAGTTCATCCTCTAAAAAAAAAAA
  AAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022916.4 unedited
  GGCGATCGTATACGACTCCTATAGGGCGGCCGGCAGAACGCGGCGCGCCGGTACCGGTCC
  GGAATTCCCGGGATATCGTCGACCCACGCGTCCGGGCAGGGGGTGGTGGAGCAAGATGGC
  GGCTCATCTGTCCTACGGCCGAGTGAACCTAAACGTGTTGCGCGAGGCGGTGCGTCGCGA
  GCTGCGCGAGTTCCTGGACAAGTGCGCAGGAAGCAAGGCAATAGTTTGGGATGAATACCT
  AACTGGACCCTTTGGCCTGATTGCACAGTATTCACTATTGAAGGAACATGAAGTGGAAAA
  AATGTTCACACTTAAAGGAAATCGTTTGCCGGCAGCTGATGTGAAGAATATAATTTTTTT
  TGTCAGACCCAGGCTAGAGTTGATGGATATAATCGCTGAAAACGTGCTCAGTGAAGATAG
  ACGAGGCCCAACGAGAGATTTTCATATTCTGTTTGTGCCACGCCGTAGCCTGTTGTGCGA
  ACAGCGGTTGAAGGATCTGGGTGTCTTGGGATCCTTTATTCACAGGGAGGAGTACAGCTT
  AGATCTCATTCCATTCGATGGGGATCTCTTATCCATGGAATCAGAGGGTGCATTCAAAGA
  GTGCTACCTGGAGGGTGACCAGACGAGCCTGTACCACGCAGCCAAGGGGCTGATGACCCT
  GCAAGCTCTGTATGGAACGATCCCCCAGATCTTTGGGAAAGGAGAATGCGCTCGGCAAGT
  GGCCCAATATGATGATCAGGATGAAGAGAGAGTTTACAGGAAGCCAGAATTCAATATTTC
  CTGTTTTTGATATCTCTTGTTGCTTGATCGGAATGTGATTTATTAACACCTCTTGCCACT
  CAGCTGAACATTATGAAGACTCATTGATGAAATTTATGGCATTCAGAACAGTATTGTGAA
  ATTACCTCCAGAGAATTTGCACCCTAAGAAACCAGGGCGATGTGGTAAGGACCCTCCCCA
  CGGAAGCCAAGAAAGCTTGCAGCCTGGAATTTCTGGA
• Restriction Sites: Please inquire
• ACCN: NM_022916
• ORF Size: 1791 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_022916.4, NP_075067.2
• RefSeq Size: 2628
• RefSeq ORF: 1791
• Locus ID: 65082
• Domains: Sec1
• Gene Summary: This gene encodes a tethering protein and a core subunit of the homotypic fusion and protein sorting (HOPS) complex. The HOPS complex and a second endosomal tethering complex called the class C core vacuole/endosome tethering (CORVET) complex, perform diverse functions in endocytosis including membrane tethering, RabGTPase interaction, activation and proofreading of synaptic-soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) assembly to drive membrane fusion, and endosome-to-cytoskeleton attachment. The HOPS complex controls endosome maturation as well as endosome traffic to the lysosome. This complex is essential for vacuolar fusion and is required for adaptor protein complex 3-dependent transport from the golgi to the vacuole. The encoded protein belongs to the Sec1/Munc18 (SM) family of SNARE-mediated membrane fusion regulators. Naturally occurring mutations in this gene are associated with a novel mucopolysaccharidosis-like disease. [provided by RefSeq, Apr 2017]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.