ITM2B (NM_021999) Human Tagged ORF Clone
CAT#: RC202377
ITM2B (Myc-DDK-tagged)-Human integral membrane protein 2B (ITM2B)
"NM_021999" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ITM2B |
Synonyms | ABRI; BRI; BRI2; BRICD2B; E3-16; E25B; FBD; imBRI2; RDGCA |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202377 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAAGGTGACGTTCAACTCCGCTCTGGCCCAGAAGGAGACCAAGAAGGACGAGCCCAAGAGCGGCG AGGAGGCGCTCATCATCCCCCCCGACGCCGTCGCGGTGGACTGCAAGGACCCAGATGATGTGGTACCAGT TGGCCAAAGAAGAGCCTGGTGTTGGTGCATGTGCTTTGGACTAGCATTTATGCTTGCAGGTGTTATTCTA GGAGGAGCATACTTGTACAAATATTTTGCACTTCAACCAGATGACGTGTACTACTGTGGAATAAAGTACA TCAAAGATGATGTCATCTTAAATGAGCCCTCTGCAGATGCCCCAGCTGCTCTCTACCAGACAATTGAAGA AAATATTAAAATCTTTGAAGAAGAAGAAGTTGAATTTATCAGTGTGCCTGTCCCAGAGTTTGCAGATAGT GATCCTGCCAACATTGTTCATGACTTTAACAAGAAACTTACAGCCTATTTAGATCTTAACCTGGATAAGT GCTATGTGATCCCTCTGAACACTTCCATTGTTATGCCACCCAGAAACCTACTGGAGTTACTTATTAACAT CAAGGCTGGAACCTATTTGCCTCAGTCCTATCTGATTCATGAGCACATGGTTATTACTGATCGCATTGAA AACATTGATCACCTGGGTTTCTTTATTTATCGACTGTGTCATGACAAGGAAACTTACAAACTGCAACGCA GAGAAACTATTAAAGGTATTCAGAAACGTGAAGCCAGCAATTGTTTCGCAATTCGGCATTTTGAAAACAA ATTTGCCGTGGAAACTTTAATTTGTTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202377 protein sequence
Red=Cloning site Green=Tags(s) MVKVTFNSALAQKETKKDEPKSGEEALIIPPDAVAVDCKDPDDVVPVGQRRAWCWCMCFGLAFMLAGVIL GGAYLYKYFALQPDDVYYCGIKYIKDDVILNEPSADAPAALYQTIEENIKIFEEEEVEFISVPVPEFADS DPANIVHDFNKKLTAYLDLNLDKCYVIPLNTSIVMPPRNLLELLINIKAGTYLPQSYLIHEHMVITDRIE NIDHLGFFIYRLCHDKETYKLQRRETIKGIQKREASNCFAIRHFENKFAVETLICS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021999 |
ORF Size | 798 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021999.1, NM_021999.2, NM_021999.3, NM_021999.4, NP_068839.1 |
RefSeq Size | 1896 bp |
RefSeq ORF | 801 bp |
Locus ID | 9445 |
Domains | BRICHOS |
Protein Families | Druggable Genome, Transmembrane |
MW | 30.4 kDa |
Gene Summary | Amyloid precursor proteins are processed by beta-secretase and gamma-secretase to produce beta-amyloid peptides which form the characteristic plaques of Alzheimer disease. This gene encodes a transmembrane protein which is processed at the C-terminus by furin or furin-like proteases to produce a small secreted peptide which inhibits the deposition of beta-amyloid. Mutations which result in extension of the C-terminal end of the encoded protein, thereby increasing the size of the secreted peptide, are associated with two neurogenerative diseases, familial British dementia and familial Danish dementia. [provided by RefSeq, Oct 2009] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC108808 | ITM2B (untagged)-Human integral membrane protein 2B (ITM2B) |
USD 310.00 |
|
SC322363 | ITM2B (untagged)-Human integral membrane protein 2B (ITM2B) |
USD 310.00 |
|
RG202377 | ITM2B (GFP-tagged) - Human integral membrane protein 2B (ITM2B) |
USD 460.00 |
|
RC202377L1 | Lenti ORF clone of Human integral membrane protein 2B (ITM2B), Myc-DDK-tagged |
USD 768.00 |
|
RC202377L2 | Lenti ORF clone of Human integral membrane protein 2B (ITM2B), mGFP tagged |
USD 620.00 |
|
RC202377L3 | Lenti ORF clone of Human integral membrane protein 2B (ITM2B), Myc-DDK-tagged |
USD 620.00 |
|
RC202377L4 | Lenti ORF clone of Human integral membrane protein 2B (ITM2B), mGFP tagged |
USD 620.00 |
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