USP5 (NM_003481) Human Tagged ORF Clone

CAT#: RC202624L2

Lenti ORF clone of Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 2, mGFP tagged

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: USP5
• Synonyms: ISOT
• Vector: pLenti-C-mGFP
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC202624).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_003481
• ORF Size: 2505 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003481.1, NP_003472.1
• RefSeq Size: 3130
• RefSeq ORF: 2508
• Locus ID: 8078
• Domains: UBA, UCH, zf-UBP
• Protein Families: Druggable Genome, Protease
• MW: 93.3 kDa
• Gene Summary: Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]). [supplied by OMIM, Mar 2010]