USP5 (NM_003481) Human Untagged Clone

CAT#: SC117962

USP5 (untagged)-Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP5
• Synonyms: ISOT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003481 edited
  GGGCGGCCGCGAATTCGGCACGAGGGAGAAGCTGCTGCCGGTGTCATGGCGGAGCTGAGT
  GAGGAGGCGCTGCTGTCAGTATTACCGACGATCCGGGTCCCTAAGGCTGGAGACCGGGTC
  CACAAAGACGAGTGCGCCTTCTCCTTCGACACGCCGGAGTCTGAGGGGGGCCTCTACATC
  TGTATGAACACGTTTCTGGGCTTTGGGAAACAGTATGTGGAGAGACATTTCAATAAGACC
  GGCCAGCGAGTCTACTTGCACCTCCGGCGGACCCGGCGCCCGAAAGAGGAGGACCCTGCT
  ACAGGCACTGGAGACCCACCCCGGAAGAAGCCCACGCGGCTGGCTATTGGTGTTGAAGGC
  GGGTTTGACCTTAGCGAGGAGAAGTTTGAATTAGACGAGGATGTGAAGATTGTCATTTTG
  CCAGATTACCTGGAGATTGCCCGGGATGGACTGGGGGGACTGCCTGACATTGTCAGAGAT
  CGGGTGACCAGTGCAGTGGAGGCCCTACTGTCGGCCGACTCAGCCTCCCGCAAGCAGGAG
  GTGCAGGCATGGGATGGGGAAGTACGGCAGGTGTCTAAGCATGCCTTCAGCCTCAAGCAG
  TTGGACAACCCTGCTCGAATCCCTCCCTGTGGCTGGAAGTGCTCCAAGTGTGACATGAGA
  GAGAACCTGTGGCTCAACCTGACTGATGGCTCCATCCTCTGTGGGCGACGCTACTTCGAT
  GGCAGTGGGGGCAACAACCACGCTGTGGAGCACTACCGAGAGACAGGCTACCCGTTAGCT
  GTCAAGCTGGGCACCATCACCCCTGATGGAGCTGACGTGTACTCATATGATGAGGATGAC
  ATGGTCCTGGACCCCAGCCTGGCTGAGCACCTGTCCCACTTCGGCATCGACATGCTGAAG
  ATGCAGAAGACAGACAAGACGATGACTGAGTTGGAGATAGACATGAACCAGCGGATTGGT
  GAATGGGAGCTGATCCAGGAGTCAGGTGTGCCACTCAAGCCCCTGTTTGGGCCTGGCTAC
  ACAGGCATCCGGAACCTGGGTAACAGCTGCTACCTCAACTCTGTGGTCCAGGTGCTCTTC
  AGCATCCCTGACTTCCAGAGGAAGTATGTGGATAAGCTGGAGAAGATCTTCCAGAATGCC
  CCGACGGACCCTACCCAGGATTTCAGCACCCAGGTGGCCAAGCTGGGCCATGGCCTTCTC
  TCCGGGGAGTATTCCAAGCCAGTACCGGAGTCGGGCGATGGGGAGCGGGTGCCAGAACAG
  AAGGAAGTTCAAGATGGCATTGCCCCTCGGATGTTCAAGGCCCTCATCGGCAAGGGCCAC
  CCTGAATTCTCCACCAACCGGCAGCAGGATGCCCAGGAGTTCTTCCTTCACCTTATCAAC
  ATGGTGGAGAGGAATTGCCGGAGCTCTGAAAATCCTAATGAAGTGTTCCGCTTCTTGGTG
  GAGGAAAAGATCAAGTGCCTGGCCACAGAGAAGGTGAAGTACACCCAGCGAGTTGACTAC
  ATCATGCAGCTGCCTGTGCCCATGGATGCAGCCCTTAACAAAGAGGAGCTTCTGGAGTAC
  GAGGAGAAGAAGCGGCAAGCCGAAGAGGAGAAGATGGCACTGCCAGAACTGGTTCGGGCC
  CAGGTGCCCTTCAGCTCTTGCCTGGAGGCCTACGGGGCCCCTGAGCAGGTCGATGACTTC
  TGGAGCACGGCCCTGCAGGCCAAGTCAGTAGCTGTCAAGACCACACGATTTGCCTCATTC
  CCTGACTACCTGGTCATCCAGATCAAGAAGTTCACCTTCGGCTTAGACTGGGTGCCCAAG
  AAACTGGATGTGTCCATCGAGATGCCAGAGGAGCTCGACATCTCCCAGTTGAGGGGCACA
  GGGCTGCAGCCCGGAGAGGAGGAGCTGCCAGACATTGCCCCACCCCTGGTCACTCCGGAT
  GAGCCCAAAGCGCCCATGCTGGATGAATCAGTCATCATCCAGCTGGTGGAGATGGGATTC
  CCTATGGACGCCTGCCGCAAAGCTGTCTACTACACGGGCAACAGCGGGGCTGAGGCCGCC
  ATGAACTGGGTCATGTCACACATGGATGATCCAGATTTTGCAAACCCCCTCATCCTGCCT
  GGCTCTAGTGGGCCGGGCTCCACAAGCGCAGCAGCCGACCCCCCTCCTGAGGACTGTGTG
  ACCACCATTGTCTCCATGGGCTTCTCCCGGGACCAGGCCTTGAAAGCGCTGCGGGCCACG
  AACAATAGTTTAGAACGGGCTGTGGACTGGATCTTCAGTCACATTGACGACCTGGATGCT
  GAAGCTGCCATGGACATCTCAGAGGGCCGCTCAGCTGCCGACTCCATCTCTGAGTCTGTG
  CCAGTGGGACCTAAAGTCCGGGATGGTCCTGGAAAGTATCAGCTCTTTGCCTTCATTAGT
  CACATGGGCACCTCTACCATGTGTGGTCACTACGTCTGCCACATCAAGAAAGAAGGCAGA
  TGGGTGATCTACAATGACCAGAAAGTGTGTGCCTCCGAGAAGCCGCCCAAGGACCTGGGC
  TACATCTACTTCTACCAGAGAGTGGCCAGCTAAGAGCCTGCCTCACCCCTTACCAATGAG
  GGCAGGGGAAGACCACCTGGCATGAGGGAGAGGGGCTGAGGGATGGACTTCAGCCCCTCT
  GCTCTGTACCCTTTTTCCTTTTGTCCCCGGCAGCAGGGAAGAAGCTGGAGGCCGTGGGAG
  AATGGCTGGGCAGAGCAGAGGGGCAGCGATAGACTCTGGGGATGGAGCAGGACGGGGACG
  GGAGGGGCCGGCCACCTGTCTGTAAGGAGACTTTGTTGCTTCCCCTGCCCCCGGAATCCA
  CAGTGCTCTGCTTCTCTGTGTCGCCCCGCCCAGCCCCCTGGTGTGGAGGGAGGGGTCTCG
  TTTGTGCGCGTGGGTGTAGCTTTGTGCATCCTCTCCCAGTGGAGCGATCACCTGTGCCTC
  CCCTCCCCCTTTGTTTGCCCCTGTGTGGTTGGTCAAGGAGGGATGTGAGGGAAATAGGGA
  CCCCCCGACTTGCCCTCCTGCCTCAGTCTTTCCCCCACCCTGTCTCTTCCTTGTCCTTCT
  CTGGAAAATGCCAAAATACACGATGTGAATAAAAGTACAACGGCTAAAAAAAAAAAAAAA
  AAAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003481 unedited
  ATATTTGTATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGNAGAAGCTGCTGC
  CGGTGTCATGGCGGAGCTGAGTGAGGAGGCGCTGCTGTCAGTATTACCGACGATCCGGGT
  CCCTAAGGCTGGAGACCGGGTCCACAAAGACGAGTGCGCCTTCTCCTTCGACACGCCGGA
  GTCTGAGGGGGGCCTCTACATCTGTATGAACACGTTTCTGGGCTTTGGGAAACAGTATGT
  GGAGAGACATTTCAATAAGACCGGCCAGCGAGTCTACTTGCACCTCCGGCGGACCCGGCG
  CCCGAAAGAGGAGGACCCTGCTACAGGCACTGGAGACCCACCCCGGAAGAAGCCCACGCG
  GCTGGCTATTGGTGTTGAAGGCGGGTTTGACCTTAGCGAGGAGAAGTTTGAATTAGACGA
  GGATGTGAAGATTGTCATTTTGCCAGATTACCTGGAGATTGCCCGGGATGGACTGGGGGG
  ACTGCCTGACATTGTCAGAGATCGGGTGACCAGTGCAGTGGAGGCCCTACTGTCGGCCGA
  CTCAGCCTCCCGCAAGCAGGAGGTGCAGGCATGGGATGGGGAAGTACGGCAGGTGTCTAA
  GCATGCCTTCAGCCTCAAGCAGTTGGACAACCCTGCTCGAATCCCTCCCTGTGNGCTGGA
  AGTGCTCCAAGTGTGACATGAGAGAGAACCTGTGGCTCAACCTGACTGATGGCTCCATCC
  TCTGTGGGCGACGCTACTTCGATGGCAGTGGNGGCAACACCACGCTGTGGAGCACTACCG
  AGAGACGGCTACCCGTTAGCTGTCAAGCTGGGCACCATCACCCCTGATGGGAGCTGACGT
  GTACTNCATATGATGAGGATTGACATGGGTCCCTGGACNCCCAGCCTGGNCTGAGCACCC
  TGTCCACTTNNCGCATCGACATGGCTGAAGAATGCNNAAGACAGACAAGAACGATGACTG
  AGNTGGNAGAAGACATGACCAGCNGNATNNNGTGATGGNGNAGCTGACN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003481 unedited
  CTATGGACCGCGCGCCGCTATCTANGATCGAGTTTTTTTTTTTTTTTTTTAGCCGTTGTA
  CTTTTATTCACATCGTGTATTTTGGCATTTTCCAGAGAAGGACAAGGAAGAGACAGGGTG
  GGGGAAAGACTGAGGCAGGAGGGCAAGTCGGGGGGTCCCTATTTCCCTCACATCCCTCCT
  TGACCAACCACACAGGGGCAAACAAAGGGGGAGGGGAGGCACAGGTGATCGCTCCACTGG
  GAGAGGATGCACAAAGCTACACCCACGCGCACAAACGAGACCCCTCCCTCCACACCAGGG
  GGCTGGGCGGGGCGACACAGAGAAGCAGAGCACTGTGGATTCCGGGGGCAGGGGAAGCAA
  CAAAGTCTCCTTACAGACAGGTGGCCGGCCCCTCCCGTCCCCGTCCTGCTCCATCCCCAG
  AGTCTATCGCTGCCCCTCTGCTCTGCCCAGCCATTCTCCCACGGCCTCCAGCTTCTTCCC
  TGCTGCCGGGGACAAAAGGAAAAAGGGTACAGAGCAGAGGGGCTGAAGTCCATCCCTCAG
  CCCCTCTCCCTCATGCCAGGTGGTCTTCCCCTGCCCTCATTGGTAAGGGGTGAGGCAGGC
  TCTTAGCTGGCCACTCTCTGGTAGAAGTAGATGTAGCCCAGGTCCTTGGGCGGCTTCTCG
  GAGGCACACACTTTCTGGTCATTGTAGATCACCCATCTGCCTTCTTTCTTGATGTGGCAG
  ACGTAGTGACCACACATGGTAGAGGTGCCCATGTGACTAATGAAGGCAAAGAGCTGATAC
  TTTNCAGGACCATCCCGGACTTTANGTCCCACTGGCACAGACTCANAGATGGAGTCGGCA
  GCTGAGCGGCCTCTGAGATGTCATGCAGCTTCACATCCAGTCGTCATGTGACTGAAGATC
  ATCCCAGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003481
• ORF Size: 2508 bp
• Insert Size: 3000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_003481.1, NP_003472.1
• RefSeq Size: 3102
• RefSeq ORF: 2508
• Locus ID: 8078
• Domains: UBA, UCH, zf-UBP
• Protein Families: Druggable Genome, Protease
• Gene Summary: Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]). [supplied by OMIM, Mar 2010]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2).