SPHK2 (NM_020126) Human Tagged ORF Clone

CAT#: RC203019

SPHK2 (Myc-DDK-tagged)-Human sphingosine kinase 2 (SPHK2), transcript variant 1

Product Images

Coomassie blue staining of purified SPHK2 protein (Cat# TP303019). The protein was produced from HEK293T cells transfected with SPHK2 cDNA clone (Cat# RC203019) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPHK2
• Synonyms: SK-2; SK 2; SPK-2; SPK 2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203019 representing NM_020126
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGACACCTTGAAGCAGAGGAGCAGCAGGACCAGAGGCCAGACCAGGAGCTGACCGGGAGCTGGG
  GCCACGGGCCTAGGAGCACCCTGGTCAGGGCTAAGGCCATGGCCCCGCCCCCACCGCCACTGGCTGCCAG
  CACCCCGCTCCTCCATGGCGAGTTTGGCTCCTACCCAGCCCGAGGCCCACGCTTTGCCCTCACCCTTACA
  TCGCAGGCCCTGCACATACAGCGGCTGCGCCCCAAACCTGAAGCCAGGCCCCGGGGTGGCCTGGTCCCGT
  TGGCCGAGGTCTCAGGCTGCTGCACCCTGCGAAGCCGCAGCCCCTCAGACTCAGCGGCCTACTTCTGCAT
  CTACACCTACCCTCGGGGCCGGCGCGGGGCCCGGCGCAGAGCCACTCGCACCTTCCGGGCAGATGGGGCC
  GCCACCTACGAAGAGAACCGTGCCGAGGCCCAGCGCTGGGCCACTGCCCTCACCTGTCTGCTCCGAGGAC
  TGCCACTGCCCGGGGATGGGGAGATCACCCCTGACCTGCTACCTCGGCCGCCCCGGTTGCTTCTATTGGT
  CAATCCCTTTGGGGGTCGGGGCCTGGCCTGGCAGTGGTGTAAGAACCACGTGCTTCCCATGATCTCTGAA
  GCTGGGCTGTCCTTCAACCTCATCCAGACAGAACGACAGAACCACGCCCGGGAGCTGGTCCAGGGGCTGA
  GCCTGAGTGAGTGGGATGGCATCGTCACGGTCTCGGGAGACGGGCTGCTCCATGAGGTGCTGAACGGGCT
  CCTAGATCGCCCTGACTGGGAGGAAGCTGTGAAGATGCCTGTGGGCATCCTCCCCTGCGGCTCGGGCAAC
  GCGCTGGCCGGAGCAGTGAACCAGCACGGGGGATTTGAGCCAGCCCTGGGCCTCGACCTGTTGCTCAACT
  GCTCACTGTTGCTGTGCCGGGGTGGTGGCCACCCACTGGACCTGCTCTCCGTGACGCTGGCCTCGGGCTC
  CCGCTGTTTCTCCTTCCTGTCTGTGGCCTGGGGCTTCGTGTCAGATGTGGATATCCAGAGCGAGCGCTTC
  AGGGCCTTGGGCAGTGCCCGCTTCACACTGGGCACGGTGCTGGGCCTCGCCACACTGCACACCTACCGCG
  GACGCCTCTCCTACCTCCCCGCCACTGTGGAACCTGCCTCGCCCACCCCTGCCCATAGCCTGCCTCGTGC
  CAAGTCGGAGCTGACCCTAACCCCAGACCCAGCCCCGCCCATGGCCCACTCACCCCTGCATCGTTCTGTG
  TCTGACCTGCCTCTTCCCCTGCCCCAGCCTGCCCTGGCCTCTCCTGGCTCGCCAGAACCCCTGCCCATCC
  TGTCCCTCAACGGTGGGGGCCCAGAGCTGGCTGGGGACTGGGGTGGGGCTGGGGATGCTCCGCTGTCCCC
  GGACCCACTGCTGTCTTCACCTCCTGGCTCTCCCAAGGCAGCTCTACACTCACCCGTCTCCGAAGGGGCC
  CCCGTAATTCCCCCATCCTCTGGGCTCCCACTTCCCACCCCTGATGCCCGGGTAGGGGCCTCCACCTGCG
  GCCCGCCCGACCACCTGCTGCCTCCGCTGGGCACCCCGCTGCCCCCAGACTGGGTGACGCTGGAGGGGGA
  CTTTGTGCTCATGTTGGCCATCTCGCCCAGCCACCTAGGCGCTGACCTGGTGGCAGCTCCGCATGCGCGC
  TTCGACGACGGCCTGGTGCACCTGTGCTGGGTGCGTAGCGGCATCTCGCGGGCTGCGCTGCTGCGCCTTT
  TCTTGGCCATGGAGCGTGGTAGCCACTTCAGCCTGGGCTGTCCGCAGCTGGGCTACGCCGCGGCCCGTGC
  CTTCCGCCTAGAGCCGCTCACACCACGCGGCGTGCTCACAGTGGACGGGGAGCAGGTGGAGTATGGGCCG
  CTACAGGCACAGATGCACCCTGGCATCGGTACACTGCTCACTGGGCCTCCTGGCTGCCCGGGGCGGGAGC
  CC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203019 representing NM_020126
  Red=Cloning site Green=Tags(s)
  
  MNGHLEAEEQQDQRPDQELTGSWGHGPRSTLVRAKAMAPPPPPLAASTPLLHGEFGSYPARGPRFALTLT
  SQALHIQRLRPKPEARPRGGLVPLAEVSGCCTLRSRSPSDSAAYFCIYTYPRGRRGARRRATRTFRADGA
  ATYEENRAEAQRWATALTCLLRGLPLPGDGEITPDLLPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISE
  AGLSFNLIQTERQNHARELVQGLSLSEWDGIVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGILPCGSGN
  ALAGAVNQHGGFEPALGLDLLLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFLSVAWGFVSDVDIQSERF
  RALGSARFTLGTVLGLATLHTYRGRLSYLPATVEPASPTPAHSLPRAKSELTLTPDPAPPMAHSPLHRSV
  SDLPLPLPQPALASPGSPEPLPILSLNGGGPELAGDWGGAGDAPLSPDPLLSSPPGSPKAALHSPVSEGA
  PVIPPSSGLPLPTPDARVGASTCGPPDHLLPPLGTPLPPDWVTLEGDFVLMLAISPSHLGADLVAAPHAR
  FDDGLVHLCWVRSGISRAALLRLFLAMERGSHFSLGCPQLGYAAARAFRLEPLTPRGVLTVDGEQVEYGP
  LQAQMHPGIGTLLTGPPGCPGREP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020126
• ORF Size: 1962 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020126.3, NM_020126.4, NP_064511.2
• RefSeq Size: 3012
• RefSeq ORF: 1965
• Locus ID: 56848
• Domains: DAGKc
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Fc gamma R-mediated phagocytosis, Metabolic pathways, Sphingolipid metabolism, VEGF signaling pathway
• MW: 69 kDa
• Gene Summary: This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]