SPHK2 (NM_020126) Human Untagged Clone

CAT#: SC320762

SPHK2 (untagged)-Human sphingosine kinase 2 (SPHK2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPHK2
• Synonyms: SK-2; SK 2; SPK-2; SPK 2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_020126.3 CGACTGACTAGCCGGGCGATAACGGCAGAGAGCATAGAGCGCAGGAACAAGCGCAACGTC
  CAAGAGGGAAGGGCCAGCACGTCGGGGGCCTCTCTGGCCCTACCCAGGCCGTGTTCTCGA
  TAGCTTTCCGGAAGAAAGGGATCTGGGAGCGAGATGCGTGTAGCTAGCACGATGCGTCGC
  GCGGTGACGCTCTGGCCCGACGCCGACGGCCTCTCAGTGGCTCCCGGAGGACCCGGCGGG
  CCCAGTGTTGGAGAGCTGAAGGTCAGGCCAGGACAGTGAGACCTGACTCCTTGCTCCTAC
  CAGCCTACTATGGCTTAAGACCCAGGGCCAGGGTCCCGTTGATGTAACAGAGCAGAGGAC
  CAGCAGATGAATGGACACCTTGAAGCAGAGGAGCAGCAGGACCAGAGGCCAGACCAGGAG
  CTGACCGGGAGCTGGGGCCACGGGCCTAGGAGCACCCTGGTCAGGGCTAAGGCCATGGCC
  CCGCCCCCACCGCCACTGGCTGCCAGCACCCCGCTCCTCCATGGCGAGTTTGGCTCCTAC
  CCAGCCCGAGGCCCACGCTTTGCCCTCACCCTTACATCGCAGGCCCTGCACATACAGCGG
  CTGCGCCCCAAACCTGAAGCCAGGCCCCGGGGTGGCCTGGTCCCGTTGGCCGAGGTCTCA
  GGCTGCTGCACCCTGCGAAGCCGCAGCCCCTCAGACTCAGCGGCCTACTTCTGCATCTAC
  ACCTACCCTCGGGGCCGGCGCGGGGCCCGGCGCAGAGCCACTCGCACCTTCCGGGCAGAT
  GGGGCCGCCACCTACGAAGAGAACCGTGCCGAGGCCCAGCGCTGGGCCACTGCCCTCACC
  TGTCTGCTCCGAGGACTGCCACTGCCCGGGGATGGGGAGATCACCCCTGACCTGCTACCT
  CGGCCGCCCCGGTTGCTTCTATTGGTCAATCCCTTTGGGGGTCGGGGCCTGGCCTGGCAG
  TGGTGTAAGAACCACGTGCTTCCCATGATCTCTGAAGCTGGGCTGTCCTTCAACCTCATC
  CAGACAGAACGACAGAACCACGCCCGGGAGCTGGTCCAGGGGCTGAGCCTGAGTGAGTGG
  GATGGCATCGTCACGGTCTCGGGAGACGGGCTGCTCCATGAGGTGCTGAACGGGCTCCTA
  GATCGCCCTGACTGGGAGGAAGCTGTGAAGATGCCTGTGGGCATCCTCCCCTGCGGCTCG
  GGCAACGCGCTGGCCGGAGCAGTGAACCAGCACGGGGGATTTGAGCCAGCCCTGGGCCTC
  GACCTGTTGCTCAACTGCTCACTGTTGCTGTGCCGGGGTGGTGGCCACCCACTGGACCTG
  CTCTCCGTGACGCTGGCCTCGGGCTCCCGCTGTTTCTCCTTCCTGTCTGTGGCCTGGGGC
  TTCGTGTCAGATGTGGATATCCAGAGCGAGCGCTTCAGGGCCTTGGGCAGTGCCCGCTTC
  ACACTGGGCACGGTGCTGGGCCTCGCCACACTGCACACCTACCGCGGACGCCTCTCCTAC
  CTCCCCGCCACTGTGGAACCTGCCTCGCCCACCCCTGCCCATAGCCTGCCTCGTGCCAAG
  TCGGAGCTGACCCTAACCCCAGACCCAGCCCCGCCCATGGCCCACTCACCCCTGCATCGT
  TCTGTGTCTGACCTGCCTCTTCCCCTGCCCCAGCCTGCCCTGGCCTCTCCTGGCTCGCCA
  GAACCCCTGCCCATCCTGTCCCTCAACGGTGGGGGCCCAGAGCTGGCTGGGGACTGGGGT
  GGGGCTGGGGATGCTCCGCTGTCCCCGGACCCACTGCTGTCTTCACCTCCTGGCTCTCCC
  AAGGCAGCTCTACACTCACCCGTCTCCGAAGGGGCCCCCGTAATTCCCCCATCCTCTGGG
  CTCCCACTTCCCACCCCTGATGCCCGGGTAGGGGCCTCCACCTGCGGCCCGCCCGACCAC
  CTGCTGCCTCCGCTGGGCACCCCGCTGCCCCCAGACTGGGTGACGCTGGAGGGGGACTTT
  GTGCTCATGTTGGCCATCTCGCCCAGCCACCTAGGCGCTGACCTGGTGGCAGCTCCGCAT
  GCGCGCTTCGACGACGGCCTGGTGCACCTGTGCTGGGTGCGTAGCGGCATCTCGCGGGCT
  GCGCTGCTGCGCCTTTTCTTGGCCATGGAGCGTGGTAGCCACTTCAGCCTGGGCTGTCCG
  CAGCTGGGCTACGCCGCGGCCCGTGCCTTCCGCCTAGAGCCGCTCACACCACGCGGCGTG
  CTCACAGTGGACGGGGAGCAGGTGGAGTATGGGCCGCTACAGGCACAGATGCACCCTGGC
  ATCGGTACACTGCTCACTGGGCCTCCTGGCTGCCCGGGGCGGGAGCCCTGAAACTAAACA
  AGCTTGGTACCCGCCGGGGGCGGGGCCTACATTCCAATGGGGCGGAGCCTGAGCTAGGGG
  GTGTGGCCTGGCTGCTAGAGTTGTGGTGGCAGGGGCCCTGGCCCCGTCTCAGGATTGCGC
  TCGCTTTCATGGGACCAGACGTGATGCTGGAAGGTGGGCGTCGTCACGGTTAAAGAGAAA
  TGGGCTCGTCCCGAGGGTAGTGCCTGATCAATGAGGGCGGGGCCTGGCGTCTGATCTGGG
  GCCGCCCTTACGGGGCAGGGCTCAGTCCTGACGCTTGCCACCTGCTCCTACCCGGCCAGG
  ATGGCTGAGGGCGGAGTCTATTTTACGCGTCGCCCAATGACAGGACCTGGAATGTACTGG
  CTGGGGTAGGCCTCAGTGAGTCGGCCGGTCAGGGCCCGCAGCCTCGCCCCATCCACTCCG
  GTGCCTCCATTTAGCTGGCCAATCAGCCCAGGAGGGGCAGGTTCCCCGGGGCCGGCGCTA
  GGATTTGCACTAATGTTCCTCTCCCCGCGGGTGGGGGCGGGGAAATTCATATCCCCTGTT
  CGTCTCATGCGCGTCCTCCGTCCCCAATCTAAAAAGCAATTGAAAAGGTCTATGCAATAA
  AGGCAGTCGCTTCATTCCTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020126.3 unedited
  GCGATTGTATACGACTCCTATAGGGCGGCGCGGGAATTCGGCACGAGGCGACTGACTAGC
  CGGGCGATAACGGCAGAGAGCATAGAGCGCAGGAACAAGCGCAACGTCCAAGAGGGAAGG
  GCCAGCACGTCGGGGGCCTCTCTGGCCCTACCCAGGCCGTGTTCTCGATAGCTTTCCGGA
  AGAAAGGGATCTGGGAGCGAGATGCGTGTAGCTAGCACGATGCGTCGCGCGGTGACGCTC
  TGGCCCGACGCCGACGGCCTCTCAGTGGCTCCCGGAGGACCCGGCGGGCCCAGTGTTGGA
  GAGCTGAAGGTCAGGCCAGGACAGTGAGACCTGACTCCTTGCTCCTACCAGCCTACTATG
  GCTTAAGACCCAGGGCCAGGGTCCCGTTGATGTAACAGAGCAGAGGACCAGCAGATGAAT
  GGACACCTTGAAGCAGAGGAGCAGCAGGACCAGAGGCCAGACCAGGAGCTGACCGGGAGC
  TGGGGCCACGGGCCTAGGAGCACCCTGGTCAGGGCTAAGGCCATGGCCCCGCCCCCACCG
  CCACTGGCTGCCAGCACCCCGCTCCTCCATGGCGAGTTTGGCTCCTACCCAGCCCGAGGC
  CCACGCTTTGCCCTCACCCTTACATCGCAGGCCCTGCACATACAGCGGCTGCGCCCCAAA
  CCTGAAGCCAGGCCCCGGGGTGGCCTGGTCCCGTTGGCCGAGGTCTCAGCTGCTGCACCC
  TGCGAAGCCGCAGCCCCTCAGACTCAGCGGCCTACTTCTGCATTCTACACCTACCCTCGG
  GGCCGGCGCGGGGCCCGGCGCAGGAGCCACTCCGCACCTTCCGGGCAGATGGGCCGCCAC
  TACGAGAGAAACCGTGCCGAAGCCCAGCGCTGGGCACTGCCCTCACCTGTCTGCTCGAGA
  CTGCCATGCGGGATGGGAGAATCCAGCCTGAACTGCTACCTCGGCCGCCCCCGGTGCTTT
  CTATGGTCATATTCCCATG
• Restriction Sites: Please inquire
• ACCN: NM_020126
• ORF Size: 1965 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_020126.3, NP_064511.2
• RefSeq Size: 3012
• RefSeq ORF: 1965
• Locus ID: 56848
• Domains: DAGKc
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Fc gamma R-mediated phagocytosis, Metabolic pathways, Sphingolipid metabolism, VEGF signaling pathway
• Gene Summary: This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1 and 3 both encode the same isoform (a).