Ubiquilin (UBQLN1) (NM_053067) Human Tagged ORF Clone

CAT#: RC204020

UBQLN1 (Myc-DDK-tagged)-Human ubiquilin 1 (UBQLN1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY409314) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204020 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified UBQLN1 protein (Cat# TP304020). The protein was produced from HEK293T cells transfected with UBQLN1 cDNA clone (Cat# RC204020) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UBQLN1
• Synonyms: DA41; DSK2; PLIC-1; UBQN; XDRP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204020 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGAGTGGTGAAAGCGGCGGTCCTCCGGGCTCCCAGGATAGCGCCGCCGGAGCCGAAGGTGCTG
  GCGCCCCCGCGGCCGCTGCCTCCGCGGAGCCCAAAATCATGAAAGTCACCGTGAAGACCCCGAAGGAAAA
  GGAGGAATTCGCCGTGCCCGAGAATAGCTCCGTCCAGCAGTTTAAGGAAGAAATCTCTAAACGTTTTAAA
  TCACATACTGACCAACTTGTGTTGATATTTGCTGGAAAAATTTTGAAAGATCAAGATACCTTGAGTCAGC
  ATGGAATTCATGATGGACTTACTGTTCACCTTGTCATTAAAACACAAAACAGGCCTCAGGATCATTCAGC
  TCAGCAAACAAATACAGCTGGAAGCAATGTTACTACATCATCAACTCCTAATAGTAACTCTACATCTGGT
  TCTGCTACTAGCAACCCTTTTGGTTTAGGTGGCCTTGGGGGACTTGCAGGTCTGAGTAGCTTGGGTTTGA
  ATACTACCAACTTCTCTGAACTACAGAGTCAGATGCAGCGACAACTTTTGTCTAACCCTGAAATGATGGT
  CCAGATCATGGAAAATCCCTTTGTTCAGAGCATGCTCTCAAATCCTGACCTGATGAGACAGTTAATTATG
  GCCAATCCACAAATGCAGCAGTTGATACAGAGAAATCCAGAAATTAGTCATATGTTGAATAATCCAGATA
  TAATGAGACAAACGTTGGAACTTGCCAGGAATCCAGCAATGATGCAGGAGATGATGAGGAACCAGGACCG
  AGCTTTGAGCAACCTAGAAAGCATCCCAGGGGGATATAATGCTTTAAGGCGCATGTACACAGATATTCAG
  GAACCAATGCTGAGTGCTGCACAAGAGCAGTTTGGTGGTAATCCATTTGCTTCCTTGGTGAGCAATACAT
  CCTCTGGTGAAGGTAGTCAACCTTCCCGTACAGAAAATAGAGATCCACTACCCAATCCATGGGCTCCACA
  GACTTCCCAGAGTTCATCAGCTTCCAGCGGCACTGCCAGCACTGTGGGTGGCACTACTGGTAGTACTGCC
  AGTGGCACTTCTGGGCAGAGTACTACTGCGCCAAATTTGGTGCCTGGAGTAGGAGCTAGTATGTTCAACA
  CACCAGGAATGCAGAGCTTGTTGCAACAAATAACTGAAAACCCACAACTGATGCAAAACATGTTGTCTGC
  CCCCTACATGAGAAGCATGATGCAGTCACTAAGCCAGAATCCTGACCTTGCTGCACAGATGCAGAATCCT
  GATACACTATCAGCAATGTCAAACCCTAGAGCAATGCAGGCCTTGTTACAGATTCAGCAGGGTTTACAGA
  CATTAGCAACGGAAGCCCCGGGCCTCATCCCAGGGTTTACTCCTGGCTTGGGGGCATTAGGAAGCACTGG
  AGGCTCTTCGGGAACTAATGGATCTAACGCCACACCTAGTGAAAACACAAGTCCCACAGCAGGAACCACT
  GAACCTGGACATCAGCAGTTTATTCAGCAGATGCTGCAGGCTCTTGCTGGAGTAAATCCTCAGCTACAGA
  ATCCAGAAGTCAGATTTCAGCAACAACTGGAACAACTCAGTGCAATGGGATTTTTGAACCGTGAAGCAAA
  CTTGCAAGCTCTAATAGCAACAGGAGGTGATATCAATGCAGCTATTGAAAGGTTACTGGGCTCCCAGCCA
  TCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204020 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAESGESGGPPGSQDSAAGAEGAGAPAAAASAEPKIMKVTVKTPKEKEEFAVPENSSVQQFKEEISKRFK
  SHTDQLVLIFAGKILKDQDTLSQHGIHDGLTVHLVIKTQNRPQDHSAQQTNTAGSNVTTSSTPNSNSTSG
  SATSNPFGLGGLGGLAGLSSLGLNTTNFSELQSQMQRQLLSNPEMMVQIMENPFVQSMLSNPDLMRQLIM
  ANPQMQQLIQRNPEISHMLNNPDIMRQTLELARNPAMMQEMMRNQDRALSNLESIPGGYNALRRMYTDIQ
  EPMLSAAQEQFGGNPFASLVSNTSSGEGSQPSRTENRDPLPNPWAPQTSQSSSASSGTASTVGGTTGSTA
  SGTSGQSTTAPNLVPGVGASMFNTPGMQSLLQQITENPQLMQNMLSAPYMRSMMQSLSQNPDLAAQMQNP
  DTLSAMSNPRAMQALLQIQQGLQTLATEAPGLIPGFTPGLGALGSTGGSSGTNGSNATPSENTSPTAGTT
  EPGHQQFIQQMLQALAGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQP
  S
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053067
• ORF Size: 1683 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053067.1, NM_053067.2, NP_444295.1
• RefSeq Size: 4093 bp
• RefSeq ORF: 1686 bp
• Locus ID: 29979
• Domains: UBA, UBQ, STI1
• Protein Families: Druggable Genome
• MW: 59.2 kDa
• Gene Summary: This gene encodes an ubiquitin-like protein (ubiquilin) that shares a high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain an N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to modulate accumulation of presenilin proteins, and it is found in lesions associated with Alzheimer's and Parkinson's disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]