BHMT2 (NM_017614) Human Tagged ORF Clone

CAT#: RC204661

BHMT2 (Myc-DDK-tagged)-Human betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY413675) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204661 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified BHMT2 protein (Cat# TP304661). The protein was produced from HEK293T cells transfected with BHMT2 cDNA clone (Cat# RC204661) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BHMT2
• Synonyms: FLJ20001
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204661 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCTGCTGGACGCCCGGGGGCCAAGAAGGGGATTTTGGAGCGCCTGGAGAGTGGGGAGGTTGTGA
  TTGGAGATGGCAGCTTTCTCATTACTCTGGAGAAGAGAGGCTATGTGAAGGCTGGGCTCTGGACTCCAGA
  GGCAGTGATAGAACACCCAGACGCAGTTCGTCAACTTCACATGGAATTCTTGAGAGCAGGATCAAATGTC
  ATGCAGACTTTTACCTTTTCTGCCAGTGAGGACAATATGGAAAGCAAGTGGGAAGATGTAAATGCTGCTG
  CCTGTGACCTCGCCAGGGAAGTGGCTGGCAAAGGTGATGCTTTGGTAGCAGGGGGGATCTGCCAGACATC
  AATATACAAATACCAGAAGGATGAAGCTAGAATTAAAAAACTTTTTCGACAACAGCTAGAAGTTTTTGCC
  TGGAAAAATGTGGACTTCTTGATTGCAGAGTATTTTGAGCACGTTGAAGAAGCTGTGTGGGCTGTGGAAG
  TCTTAAAAGAATCAGATAGACCCGTGGCAGTTACCATGTGCATAGGCCCAGAGGGAGACATGCATGATAT
  AACCCCCGGAGAATGTGCTGTGAGGCTGGTGAAGGCAGGGGCTTCCATCGTTGGCGTGAACTGCCGCTTT
  GGGCCCGACACCAGCTTGAAGACGATGGAGCTCATGAAGGAGGGTCTTGAGTGGGCAGGGCTGAAAGCGC
  ACCTCATGGTGCAGCCTCTGGGGTTCCACGCGCCTGACTGTGGCAAAGAGGGGTTTGTGGATCTCCCAGA
  ATATCCCTTTGGACTGGAGTCCAGAGTTGCCACCAGATGGGATATTCAAAAATACGCCAGAGAGGCCTAC
  AACCTGGGGGTCAGGTACATTGGCGGGTGCTGTGGATTTGAGCCCTACCACATCAGGGCAATTGCAGAGG
  AGCTGGCCCCAGAAAGGGGCTTTTTGCCACCAGCTTCAGAAAAACACGGCAGCTGGGGAAGTGGTTTGGA
  CATGCACACCAAACCCTGGATTAGAGCAAGGGCTCGAAGGGAGTATTGGGAGAATCTGCTGCCAGCTTCA
  GGCAGACCTTTCTGTCCTTCGCTGTCAAAGCCAGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204661 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPAGRPGAKKGILERLESGEVVIGDGSFLITLEKRGYVKAGLWTPEAVIEHPDAVRQLHMEFLRAGSNV
  MQTFTFSASEDNMESKWEDVNAAACDLAREVAGKGDALVAGGICQTSIYKYQKDEARIKKLFRQQLEVFA
  WKNVDFLIAEYFEHVEEAVWAVEVLKESDRPVAVTMCIGPEGDMHDITPGECAVRLVKAGASIVGVNCRF
  GPDTSLKTMELMKEGLEWAGLKAHLMVQPLGFHAPDCGKEGFVDLPEYPFGLESRVATRWDIQKYAREAY
  NLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWIRARARREYWENLLPAS
  GRPFCPSLSKPDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017614
• ORF Size: 1089 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017614.2, NM_017614.3, NM_017614.4, NP_060084.2
• RefSeq Size: 2651 bp
• RefSeq ORF: 1092 bp
• Locus ID: 23743
• Domains: S-methyl_trans
• MW: 40.4 kDa
• Gene Summary: Homocysteine is a sulfur-containing amino acid that plays a crucial role in methylation reactions. Transfer of the methyl group from betaine to homocysteine creates methionine, which donates the methyl group to methylate DNA, proteins, lipids, and other intracellular metabolites. The protein encoded by this gene is one of two methyl transferases that can catalyze the transfer of the methyl group from betaine to homocysteine. Anomalies in homocysteine metabolism have been implicated in disorders ranging from vascular disease to neural tube birth defects such as spina bifida. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]