SV2B (NM_014848) Human Tagged ORF Clone

CAT#: RC205345

SV2B (Myc-DDK-tagged)-Human synaptic vesicle glycoprotein 2B (SV2B), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY402380) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205345 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SV2B
• Synonyms: HsT19680
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205345 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGACTACAAGTATCAGGACAATTATGGGGGCTATGCTCCCAGTGATGGCTATTACCGCGGCAATG
  AGTCCAACCCAGAAGAAGATGCACAGAGTGATGTCACCGAAGGCCATGATGAGGAAGACGAGATCTATGA
  GGGCGAGTACCAGGGTATCCCTCACCCAGATGATGTCAAGGCCAAGCAGGCCAAGATGGCGCCCTCCAGA
  ATGGACAGCCTTCGGGGCCAGACAGACCTGATGGCTGAGAGGCTGGAAGATGAGGAGCAGTTGGCCCATC
  AGTACGAGACCATCATGGATGAGTGTGGCCATGGCCGCTTCCAGTGGATCCTCTTTTTCGTCTTGGGTTT
  GGCCCTGATGGCCGATGGGGTGGAAGTGTTCGTGGTGAGTTTTGCCCTGCCCAGTGCAGAGAAGGACATG
  TGTCTGTCCAGTTCCAAAAAAGGAATGCTAGGGATGATAGTCTACTTGGGAATGATGGCGGGCGCCTTCA
  TCCTGGGAGGCCTGGCTGATAAGCTGGGAAGGAAGCGAGTCCTCAGCATGTCTCTGGCCGTCAATGCCTC
  CTTCGCCTCCCTCTCTTCCTTCGTGCAGGGATATGGAGCCTTCCTCTTCTGCCGACTCATCTCAGGCATC
  GGTATTGGGGGTGCTCTACCGATTGTTTTTGCCTATTTTTCTGAATTCTTGTCTCGGGAGAAGCGAGGAG
  AACACCTCAGTTGGCTGGGCATCTTCTGGATGACTGGGGGCCTGTACGCATCTGCCATGGCCTGGAGCAT
  CATCCCACACTATGGCTGGGGCTTCAGCATGGGGACCAATTACCACTTCCATAGCTGGAGAGTGTTTGTC
  ATCGTCTGTGCTCTGCCCTGCACCGTGTCCATGGTGGCCCTGAAGTTCATGCCAGAGAGCCCAAGGTTTC
  TGCTAGAGATGGGCAAACATGATGAAGCCTGGATGATTCTCAAGCAAGTCCATGACACCAACATGAGAGC
  TAAGGGGACCCCAGAGAAAGTGTTCACGGTTTCCAACATCAAAACTCCCAAGCAAATGGATGAATTCATT
  GAGATCCAAAGTTCAACAGGAACCTGGTACCAGCGCTGGCTGGTCAGATTCAAGACCATTTTCAAGCAGG
  TCTGGGATAATGCCCTGTACTGTGTGATGGGGCCCTACAGAATGAATACACTGATTCTGGCCGTGGTTTG
  GTTTGCCATGGCATTCAGTTACTATGGACTGACAGTTTGGTTTCCTGATATGATCCGCTATTTTCAAGAT
  GAAGAATACAAGTCTAAAATGAAGGTGTTTTTTGGTGAGCATGTGTACGGCGCCACAATCAACTTCACGA
  TGGAAAATCAGATCCACCAACATGGGAAACTTGTGAATGATAAGTTCACAAGAATGTACTTTAAACATGT
  ACTCTTTGAGGACACATTCTTTGACGAGTGCTATTTTGAAGACGTAACATCAACAGATACCTACTTCAAA
  AATTGTACCATTGAATCAACCATCTTTTACAACACAGACCTCTACGAGCACAAGTTCATCAACTGTCGGT
  TTATCAACTCCACCTTCCTGGAGCAGAAGGAGGGCTGCCACATGGACTTGGAGCAAGATAATGACTTCCT
  GATTTACCTCGTCAGCTTCCTGGGCAGCCTGTCTGTCTTACCCGGGAACATCATTTCTGCCCTGCTCATG
  GATAGAATTGGAAGGCTCAAGATGATTGGTGGCTCCATGCTAATCTCTGCAGTCTGCTGCTTCTTCCTGT
  TTTTTGGCAACAGTGAGTCTGCAATGATCGGCTGGCAGTGCCTGTTCTGTGGGACAAGCATTGCAGCCTG
  GAATGCTCTGGATGTGATCACAGTGGAGCTGTATCCCACCAACCAGAGAGCAACAGCCTTCGGCATTCTC
  AATGGATTATGCAAATTTGGCGCCATCCTGGGAAACACCATCTTTGCTTCTTTTGTTGGGATAACCAAAG
  TGGTCCCCATCCTTCTGGCTGCTGCTTCTCTGGTTGGGGGTGGCCTGATTGCCCTTCGACTGCCAGAGAC
  TCGAGAACAGGTCCTGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205345 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDDYKYQDNYGGYAPSDGYYRGNESNPEEDAQSDVTEGHDEEDEIYEGEYQGIPHPDDVKAKQAKMAPSR
  MDSLRGQTDLMAERLEDEEQLAHQYETIMDECGHGRFQWILFFVLGLALMADGVEVFVVSFALPSAEKDM
  CLSSSKKGMLGMIVYLGMMAGAFILGGLADKLGRKRVLSMSLAVNASFASLSSFVQGYGAFLFCRLISGI
  GIGGALPIVFAYFSEFLSREKRGEHLSWLGIFWMTGGLYASAMAWSIIPHYGWGFSMGTNYHFHSWRVFV
  IVCALPCTVSMVALKFMPESPRFLLEMGKHDEAWMILKQVHDTNMRAKGTPEKVFTVSNIKTPKQMDEFI
  EIQSSTGTWYQRWLVRFKTIFKQVWDNALYCVMGPYRMNTLILAVVWFAMAFSYYGLTVWFPDMIRYFQD
  EEYKSKMKVFFGEHVYGATINFTMENQIHQHGKLVNDKFTRMYFKHVLFEDTFFDECYFEDVTSTDTYFK
  NCTIESTIFYNTDLYEHKFINCRFINSTFLEQKEGCHMDLEQDNDFLIYLVSFLGSLSVLPGNIISALLM
  DRIGRLKMIGGSMLISAVCCFFLFFGNSESAMIGWQCLFCGTSIAAWNALDVITVELYPTNQRATAFGIL
  NGLCKFGAILGNTIFASFVGITKVVPILLAAASLVGGGLIALRLPETREQVLM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014848
• ORF Size: 2049 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014848.1, NM_014848.2, NM_014848.3, NM_014848.4, NM_014848.5, NM_014848.6, NP_055663.1
• RefSeq Size: 11361 bp
• RefSeq ORF: 2052 bp
• Locus ID: 9899
• Domains: sugar_tr
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: ECM-receptor interaction
• MW: 77.4 kDa
• Gene Summary: This gene encodes a member of the synaptic vesicle proteins 2 (SV2) family and major facilitator superfamily of proteins. This protein and other members of the family are localized to synaptic vesicles and may function in the regulation of vesicle trafficking and exocytosis. Studies in mice suggest that the encoded protein may act as a protein receptor for botulinum neurotoxin E in neurons, and that this protein may be important for the integrity of the glomerular filtration barrier. This gene shows reduced expression in areas of synaptic loss in the hippocampus of human temporal lobe epilepsy patients. [provided by RefSeq, Sep 2016]