ATG4C (NM_178221) Human Tagged ORF Clone

CAT#: RC205955

ATG4C (Myc-DDK-tagged)-Human ATG4 autophagy related 4 homolog C (S. cerevisiae) (ATG4C), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY409886) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216088 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ATG4C protein (Cat# TP305955). The protein was produced from HEK293T cells transfected with ATG4C cDNA clone (Cat# RC205955) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATG4C
• Synonyms: APG4-C; APG4C; AUTL1; AUTL3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205955 representing NM_178221
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCTACAGGAACAGATGAAGTTGACAAGCTAAAAACCAAATTTATATCTGCTTGGAACAACATGA
  AATATAGTTGGGTGTTGAAAACAAAGACGTATTTTAGTAGAAATTCTCCTGTATTATTGCTTGGAAAATG
  TTACCATTTTAAATATGAAGATGAAGATAAAACGTTACCTGCAGAGTCGGGATGTACAATAGAGGATCAC
  GTAATTGCAGGAAATGTAGAAGAATTTCGTAAAGATTTCATTTCTAGAATATGGCTGACCTACAGGGAAG
  AATTCCCTCAAATAGAAGGCTCAGCTTTGACAACAGACTGTGGGTGGGGCTGCACATTGAGAACTGGCCA
  GATGCTCTTGGCTCAAGGACTCATACTACACTTTCTTGGTAGAGCTTGGACCTGGCCTGATGCTTTGAAT
  ATTGAAAATTCAGACTCTGAATCATGGACTTCCCACACTGTCAAAAAATTTACTGCATCATTTGAAGCAT
  CACTTTCAGGGGAAAGAGAATTCAAAACCCCAACAATTTCTCTGAAGGAAACAATTGGGAAATATTCTGA
  TGATCATGAAATGCGAAATGAAGTTTATCATAGGAAAATCATCTCTTGGTTTGGTGATTCCCCCTTGGCT
  CTTTTTGGCTTACATCAACTAATAGAATATGGAAAGAAGTCTGGGAAAAAAGCAGGAGATTGGTATGGAC
  CAGCTGTGGTTGCTCACATTTTAAGAAAAGCAGTTGAAGAAGCAAGGCATCCTGATTTACAAGGAATAAC
  TATTTATGTTGCACAAGATTGTACAGTTTACAATTCTGATGTAATTGATAAACAGAGTGCTTCCATGACT
  TCTGATAATGCAGATGACAAAGCTGTTATTATTCTAGTTCCTGTTAGACTTGGTGGAGAAAGAACCAACA
  CCGACTACTTAGAATTTGTGAAGGGTATTTTAAGCCTGGAATATTGTGTGGGTATTATTGGTGGCAAACC
  TAAACAGTCATATTACTTTGCTGGATTTCAAGATGACAGTTTGATTTACATGGATCCTCATTACTGCCAA
  TCTTTTGTAGATGTCAGCATAAAGGATTTCCCTCTTGAGACATTCCACTGCCCTTCTCCCAAAAAGATGT
  CTTTTCGAAAAATGGATCCCAGCTGTACAATAGGATTTTACTGTCGAAATGTTCAGGACTTCAAACGAGC
  TTCTGAAGAAATCACCAAGATGCTGAAATTTTCTTCTAAGGAGAAATATCCCTTATTTACTTTTGTAAAT
  GGTCATTCCAGAGACTATGATTTTACATCTACTACAACCAATGAAGAAGACCTTTTTTCAGAGGATGAAA
  AGAAACAATTAAAAAGATTTAGCACGGAAGAGTTTGTCTTGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205955 representing NM_178221
  Red=Cloning site Green=Tags(s)
  
  MEATGTDEVDKLKTKFISAWNNMKYSWVLKTKTYFSRNSPVLLLGKCYHFKYEDEDKTLPAESGCTIEDH
  VIAGNVEEFRKDFISRIWLTYREEFPQIEGSALTTDCGWGCTLRTGQMLLAQGLILHFLGRAWTWPDALN
  IENSDSESWTSHTVKKFTASFEASLSGEREFKTPTISLKETIGKYSDDHEMRNEVYHRKIISWFGDSPLA
  LFGLHQLIEYGKKSGKKAGDWYGPAVVAHILRKAVEEARHPDLQGITIYVAQDCTVYNSDVIDKQSASMT
  SDNADDKAVIILVPVRLGGERTNTDYLEFVKGILSLEYCVGIIGGKPKQSYYFAGFQDDSLIYMDPHYCQ
  SFVDVSIKDFPLETFHCPSPKKMSFRKMDPSCTIGFYCRNVQDFKRASEEITKMLKFSSKEKYPLFTFVN
  GHSRDYDFTSTTTNEEDLFSEDEKKQLKRFSTEEFVLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178221
• ORF Size: 1374 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178221.1, NM_178221.2, NP_835739.1
• RefSeq Size: 1774 bp
• RefSeq ORF: 1377 bp
• Locus ID: 84938
• Protein Families: Protease
• Protein Pathways: Regulation of autophagy
• MW: 52.3 kDa
• Gene Summary: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq, Jul 2008]