Kaiso (ZBTB33) (NM_006777) Human Tagged ORF Clone

CAT#: RC207287

ZBTB33 (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 33 (ZBTB33), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY416422) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207287 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZBTB33
• Synonyms: ZNF-kaiso; ZNF348
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207287 representing NM_006777
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGTAGAAAACTGATTTCTGCTACAGACATTCAGTACTCTGGCAGTCTGCTGAACTCCTTGAATG
  AGCAACGTGGCCATGGACTCTTCTGTGATGTTACCGTTATTGTGGAAGACCGAAAATTCCGGGCTCACAA
  GAATATTCTTTCAGCTTCTAGTACCTACTTCCATCAGCTCTTCTCTGTTGCTGGGCAAGTTGTTGAACTG
  AGCTTTATAAGAGCAGAGATCTTTGCAGAAATTCTCAATTATATCTATAGTTCTAAAATTGTTCGTGTTA
  GATCAGATTTGCTTGATGAGTTAATTAAATCAGGGCAGTTATTAGGAGTGAAATTTATAGCAGAGCTTGG
  TGTCCCATTGTCACAGGTTAAAAGCATCTCAGGTACAGCGCAGGATGGTAATACTGAGCCTTTACCTCCT
  GATTCTGGTGACAAGAACCTTGTAATACAGAAATCAAAAGATGAAGCCCAAGATAATGGGGCTACTATAA
  TGCCTATTATAACAGAGTCTTTTTCATTATCTGCCGAAGATTATGAAATGAAAAAGATCATTGTTACCGA
  TTCTGATGATGATGATGATGATGATGTCATTTTTTGCTCCGAGATTCTGCCCACAAAGGAGACTTTGCCG
  AGTAATAACACAGTGGCACAGGTCCAATCTAACCCAGGCCCTGTTGCTATTTCAGATGTTGCACCTAGTG
  CTAGCAATAACTCGCCCCCTTTAACAAATATCACACCTACTCAGAAACTTCCTACTCCTGTGAATCAGGC
  AACTTTGAGCCAAACACAAGGAAGTGAAAAATTGTTGGTATCTTCAGCTCCAACACATCTGACTCCCAAT
  ATTATTTTGTTAAATCAGACACCACTTTCTACACCACCAAATGTCAGTTCTTCACTTCCAAATCATATGC
  CCTCTTCAATCAATTTACTTGTGCAGAATCAGCAGACACCAAACAGTGCTATTTTAACAGGAAACAAGGC
  CAATGAAGAGGAGGAGGAGGAAATAATAGATGATGATGATGACACTATTAGCTCCAGTCCTGACTCGGCC
  GTCAGTAATACATCTTTGGTCCCACAGGCTGATACCTCCCAAAATACCAGTTTTGATGGATCATTAATAC
  AGAAGATGCAGATTCCTACACTTCTTCAAGAACCACTTTCCAATTCCTTAAAAATTTCAGATATAATTAC
  TAGAAATACTAATGATCCAGGCGTAGGATCAAAACATCTAATGGAGGGTCAGAAGATCATTACTTTAGAT
  ACAGCTACTGAAATTGAAGGCTTATCGACTGGTTGCAAGGTTTATGCAAATATCGGTGAAGATACTTATG
  ATATAGTGATCCCTGTCAAAGATGACCCTGATGAAGGGGAGGCCAGACTTGAGAATGAAATACCAAAAAC
  GTCTGGCAGCGAGATGGCAAACAAACGTATGAAAGTAAAACATGATGATCACTATGAGTTAATAGTAGAT
  GGAAGGGTCTATTATATCTGTATTGTATGCAAAAGGTCATATGTCTGTCTGACAAGCTTGCGGAGACATT
  TTAACATTCATTCTTGGGAGAAGAAGTATCCGTGCCGTTACTGTGAGAAGGTATTTCCTCTTGCAGAATA
  TCGCACAAAGCATGAAATTCATCACACAGGGGAGCGAAGGTATCAGTGTTTGGCCTGTGGCAAATCTTTC
  ATCAACTATCAGTTTATGTCTTCACATATAAAGTCAGTTCATAGTCAAGATCCTTCTGGGGACTCAAAGC
  TTTATCGTTTACATCCATGCAGGTCTTTACAAATCAGACAATATGCATATCTTTCCGATAGATCAAGCAC
  TATTCCTGCAATGAAGGATGATGGTATTGGGTATAAGGTTGACACTGGAAAAGAACCTCCAGTAGGGACC
  ACTACATCTACTCAGAACAAGCCAATGACCTGGGAAGATATTTTTATTCAGCAGGAAAATGATTCAATTT
  TTAAACAAAATGTAACAGATGGCAGTACTGAGTTTGAATTTATAATACCAGAGTCTTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207287 representing NM_006777
  Red=Cloning site Green=Tags(s)
  
  MESRKLISATDIQYSGSLLNSLNEQRGHGLFCDVTVIVEDRKFRAHKNILSASSTYFHQLFSVAGQVVEL
  SFIRAEIFAEILNYIYSSKIVRVRSDLLDELIKSGQLLGVKFIAELGVPLSQVKSISGTAQDGNTEPLPP
  DSGDKNLVIQKSKDEAQDNGATIMPIITESFSLSAEDYEMKKIIVTDSDDDDDDDVIFCSEILPTKETLP
  SNNTVAQVQSNPGPVAISDVAPSASNNSPPLTNITPTQKLPTPVNQATLSQTQGSEKLLVSSAPTHLTPN
  IILLNQTPLSTPPNVSSSLPNHMPSSINLLVQNQQTPNSAILTGNKANEEEEEEIIDDDDDTISSSPDSA
  VSNTSLVPQADTSQNTSFDGSLIQKMQIPTLLQEPLSNSLKISDIITRNTNDPGVGSKHLMEGQKIITLD
  TATEIEGLSTGCKVYANIGEDTYDIVIPVKDDPDEGEARLENEIPKTSGSEMANKRMKVKHDDHYELIVD
  GRVYYICIVCKRSYVCLTSLRRHFNIHSWEKKYPCRYCEKVFPLAEYRTKHEIHHTGERRYQCLACGKSF
  INYQFMSSHIKSVHSQDPSGDSKLYRLHPCRSLQIRQYAYLSDRSSTIPAMKDDGIGYKVDTGKEPPVGT
  TTSTQNKPMTWEDIFIQQENDSIFKQNVTDGSTEFEFIIPESY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006777
• ORF Size: 2019 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006777.2, NM_006777.3, NP_006768.1
• RefSeq Size: 5225
• RefSeq ORF: 2019
• Locus ID: 10009
• Domains: BTB, zf-C2H2
• MW: 74.3 kDa
• Gene Summary: This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-α2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, May 2010]