CysLT1 (CYSLTR1) (NM_006639) Human Tagged ORF Clone

CAT#: RC207763

CYSLTR1 (Myc-DDK-tagged)-Human cysteinyl leukotriene receptor 1 (CYSLTR1)

Product Images

Western validation with an anti-DDK antibody* L: Control HEK293 lysate R: Over-expression lysate

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CYSLTR1
• Synonyms: CYSLT1; CYSLT1R; CYSLTR; HMTMF81
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207763 representing NM_006639
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAAACAGGAAATCTGACAGTATCTTCTGCCACATGCCATGACACTATTGATGACTTCCGCAATC
  AAGTGTATTCCACCTTGTACTCTATGATCTCTGTTGTAGGCTTCTTTGGCAATGGCTTTGTGCTCTATGT
  CCTCATAAAAACCTATCACAAGAAGTCAGCCTTCCAAGTATACATGATTAATTTAGCAGTAGCAGATCTA
  CTTTGTGTGTGCACACTGCCTCTCCGTGTGGTCTATTATGTTCACAAAGGCATTTGGCTCTTTGGTGACT
  TCTTGTGCCGCCTCAGCACCTATGCTTTGTATGTCAACCTCTATTGTAGCATCTTCTTTATGACAGCCAT
  GAGCTTTTTCCGGTGCATTGCAATTGTTTTTCCAGTCCAGAACATTAATTTGGTTACACAGAAAAAAGCC
  AGGTTTGTGTGTGTAGGTATTTGGATTTTTGTGATTTTGACCAGTTCTCCATTTCTAATGGCCAAACCAC
  AAAAAGATGAGAAAAATAATACCAAGTGCTTTGAGCCCCCACAAGACAATCAAACTAAAAATCATGTTTT
  GGTCTTGCATTATGTGTCATTGTTTGTTGGCTTTATCATCCCTTTTGTTATTATAATTGTCTGTTACACA
  ATGATCATTTTGACCTTACTAAAAAAATCAATGAAAAAAAATCTGTCAAGTCATAAAAAGGCTATAGGAA
  TGATCATGGTCGTGACCGCTGCCTTTTTAGTCAGTTTCATGCCATATCATATTCAACGTACCATTCACCT
  TCATTTTTTACACAATGAAACTAAACCCTGTGATTCTGTCCTTAGAATGCAGAAGTCCGTGGTCATAACC
  TTGTCTCTGGCTGCATCCAATTGTTGCTTTGACCCTCTCCTATATTTCTTTTCTGGGGGTAACTTTAGGA
  AAAGGCTGTCTACATTCAGAAAGCATTCTTTGTCCAGCGTGACTTATGTACCCAGAAAGAAGGCCTCTTT
  GCCAGAAAAAGGAGAAGAAATATGTAAAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207763 representing NM_006639
  Red=Cloning site Green=Tags(s)
  
  MDETGNLTVSSATCHDTIDDFRNQVYSTLYSMISVVGFFGNGFVLYVLIKTYHKKSAFQVYMINLAVADL
  LCVCTLPLRVVYYVHKGIWLFGDFLCRLSTYALYVNLYCSIFFMTAMSFFRCIAIVFPVQNINLVTQKKA
  RFVCVGIWIFVILTSSPFLMAKPQKDEKNNTKCFEPPQDNQTKNHVLVLHYVSLFVGFIIPFVIIIVCYT
  MIILTLLKKSMKKNLSSHKKAIGMIMVVTAAFLVSFMPYHIQRTIHLHFLHNETKPCDSVLRMQKSVVIT
  LSLAASNCCFDPLLYFFSGGNFRKRLSTFRKHSLSSVTYVPRKKASLPEKGEEICKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006639
• ORF Size: 1011 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_006639.1, NM_006639.2, NM_006639.3
• RefSeq Size: 1537 bp
• RefSeq ORF: 1014 bp
• Locus ID: 10800
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• MW: 38.4 kDa
• Gene Summary: This gene encodes a member of the G-protein coupled receptor 1 family. The encoded protein is a receptor for cysteinyl leukotrienes, and is involved in mediating bronchoconstriction via activation of a phosphatidylinositol-calcium second messenger system. Activation of the encoded receptor results in contraction and proliferation of bronchial smooth muscle cells, eosinophil migration, and damage to the mucus layer in the lung. Upregulation of this gene is associated with asthma and dysregulation may also be implicated in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]