PHOX2A (NM_005169) Human Tagged ORF Clone

CAT#: RC208331

PHOX2A (Myc-DDK-tagged)-Human paired-like homeobox 2a (PHOX2A)

Product Images

Western blot validation of overexpression lysate (Cat# LY417472) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208331 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PHOX2A
• Synonyms: ARIX; CFEOM2; FEOM2; NCAM2; PMX2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208331 representing NM_005169
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTACTCCTACCTCAATTCGTACGACTCGTGCGTGGCGGCCATGGAGGCGTCCGCCTACGGCGACT
  TTGGCGCCTGCAGCCAGCCCGGCGGCTTCCAATACAGCCCCCTGCGGCCCGCTTTCCCCGCGGCAGGGCC
  GCCCTGCCCCGCGCTCGGCTCCTCCAACTGCGCACTTGGCGCCCTACGCGACCACCAGCCCGCGCCCTAC
  TCGGCAGTGCCCTACAAGTTCTTCCCAGAGCCATCCGGCCTGCACGAGAAGCGCAAGCAGCGGCGCATCC
  GCACCACGTTCACCAGCGCGCAGCTCAAGGAGCTGGAGCGCGTTTTCGCTGAGACCCACTACCCCGACAT
  TTACACGCGTGAGGAGCTGGCGCTCAAGATCGACCTCACTGAGGCTCGCGTGCAGGTCTGGTTCCAGAAC
  CGCCGGGCCAAGTTCCGCAAACAGGAGCGCGCGGCCAGCGCCAAGGGCGCGGCGGGCGCGGCGGGCGCCA
  AAAAGGGCGAGGCGCGCTGCTCCTCCGAGGACGACGATTCCAAGGAGTCCACGTGCAGCCCCACGCCCGA
  TAGCACCGCCTCGCTGCCGCCGCCGCCTGCGCCCGGCCTGGCCAGCCCGCGCCTGAGCCCCAGCCCGCTG
  CCCGTCGCACTGGGCTCCGGGCCGGGACCTGGGCCGGGGCCACAGCCGCTCAAGGGCGCACTGTGGGCCG
  GTGTGGCGGGCGGTGGGGGCGGCGGGCCTGGCGCGGGAGCGGCCGAACTACTTAAGGCTTGGCAGCCGGC
  GGAGTCCGGCCCCGGGCCCTTCTCCGGGGTTCTGTCCTCCTTTCACCGGAAGCCCGGCCCCGCCCTGAAG
  ACCAATCTCTTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208331 representing NM_005169
  Red=Cloning site Green=Tags(s)
  
  MDYSYLNSYDSCVAAMEASAYGDFGACSQPGGFQYSPLRPAFPAAGPPCPALGSSNCALGALRDHQPAPY
  SAVPYKFFPEPSGLHEKRKQRRIRTTFTSAQLKELERVFAETHYPDIYTREELALKIDLTEARVQVWFQN
  RRAKFRKQERAASAKGAAGAAGAKKGEARCSSEDDDSKESTCSPTPDSTASLPPPPAPGLASPRLSPSPL
  PVALGSGPGPGPGPQPLKGALWAGVAGGGGGGPGAGAAELLKAWQPAESGPGPFSGVLSSFHRKPGPALK
  TNLF
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005169
• ORF Size: 852 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005169.2, NM_005169.3, NP_005160.2
• RefSeq Size: 1716 bp
• RefSeq ORF: 855 bp
• Locus ID: 401
• Cytogenetics: 11q13.4
• Protein Families: Transcription Factors
• MW: 29.7 kDa
• Gene Summary: 'The protein encoded by this gene contains a paired-like homeodomain most similar to that of the Drosophila aristaless gene product. The encoded protein plays a central role in development of the autonomic nervous system. It regulates the expression of tyrosine hydroxylase and dopamine beta-hydroxylase, two catecholaminergic biosynthetic enzymes essential for the differentiation and maintenance of the noradrenergic neurotransmitter phenotype. The encoded protein has also been shown to regulate transcription of the alpha3 nicotinic acetylcholine receptor gene. Mutations in this gene have been associated with autosomal recessive congenital fibrosis of the extraocular muscles. [provided by RefSeq, Jul 2008]'