Hexokinase II (HK2) (NM_000189) Human Tagged ORF Clone
CAT#: RC209482L3
- LentiORF®
Lenti ORF clone of Human hexokinase 2 (HK2), Myc-DDK-tagged
"NM_000189" in other vectors (6)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | HK2 |
Synonyms | HKII; HXK2 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC209482).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_000189 |
ORF Size | 2751 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000189.4, NP_000180.2 |
RefSeq Size | 7109 bp |
RefSeq ORF | 2754 bp |
Locus ID | 3099 |
Cytogenetics | 2p12 |
Domains | hexokinase |
Protein Families | Druggable Genome |
Protein Pathways | Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Metabolic pathways, Starch and sucrose metabolism, Type II diabetes mellitus |
MW | 102.2 kDa |
Gene Summary | 'Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC309063 | HK2 (untagged)-Human hexokinase 2 (HK2) |
USD 760.00 |
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RC209482 | HK2 (Myc-DDK-tagged)-Human hexokinase 2 (HK2) |
USD 420.00 |
|
RG209482 | HK2 (GFP-tagged) - Human hexokinase 2 (HK2) |
USD 460.00 |
|
RC209482L1 | Lenti ORF clone of Human hexokinase 2 (HK2), Myc-DDK-tagged |
USD 768.00 |
|
RC209482L2 | Lenti ORF clone of Human hexokinase 2 (HK2), mGFP tagged |
USD 620.00 |
|
RC209482L4 | Lenti ORF clone of Human hexokinase 2 (HK2), mGFP tagged |
USD 620.00 |
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