Hexokinase II (HK2) (NM_000189) Human Untagged Clone

CAT#: SC309063

HK2 (untagged)-Human hexokinase 2 (HK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HK2
• Synonyms: HKII; HXK2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000189 edited
  GAAACTCCGGCGCAGGAGTCCCGGGCTGCCGCTGGCAACATCGTGTCACCCAGCTAAGAA
  AATCCGCGGGCCCGAGCCACGCGCCTGTGAATCGGAGAGGTCCCACTGCCCGAGTGGAGC
  CGGGCTGAGATTCTTCTCAAGTTGAGCCTCAGTGATCCTGTGGCCGAAGTTAGCGCCTTG
  ACGTGGGACAACCGGACACGTCGCCAGGACAGAACTGAGGCGCCTTCTAGCAGTTGTGAC
  GCCAAAATCACGTCTCCGGAGACCCGCGCCCTCCGCCAGCCGGGCGCACCCTCGCCGGTA
  GCCTTCTTTGTGCGCCGTCCGGACTCCCAGCTCCCGGCCCGGCAGCCGAGCCCCAGCACA
  AAGCAGTCGGACCGCGCCGCCCGCCTCCCCTCTCGCGTCTCCGCCTCGGTTTCCCAACTC
  TGCGCCGTCGGGCCGCGGCAGGATGATTGCCTCGCATCTGCTTGCCTACTTCTTCACGGA
  GCTCAACCATGACCAAGTGCAGAAGGTTGACCAGTATCTCTACCACATGCGCCTCTCTGA
  TGAGACCCTCTTGGAGATCTCTAAGCGGTTCCGCAAGGAGATGGAGAAAGGGCTTGGAGC
  CACCACTCACCCTACTGCAGCAGTGAAGATGCTGCCCACCTTTGTGAGGTCCACTCCAGA
  TGGGACAGAACACGGAGAGTTCCTGGCTCTGGATCTTGGAGGGACCAACTTCCGTGTGCT
  TTGGGTGAAAGTAACGGACAATGGGCTCCAGAAGGTGGAGATGGAGAATCAGATCTATGC
  CATCCCTGAGGACATCATGCGAGGCAGTGGCACCCAGCTGTTTGACCACATTGCCGAATG
  CCTGGCTAACTTCATGGATAAGCTACAAATCAAAGACAAGAAGCTCCCACTGGGTTTTAC
  CTTCTCGTTCCCCTGCCACCAGACTAAACTAGACGAGAGTTTCCTGGTCTCATGGACCAA
  GGGATTCAAGTCCAGTGGAGTGGAAGGCAGAGACGTTGTGGCTCTGATCCGGAAGGCCAT
  CCAGAGGAGAGGGGACTTTGATATCGACATTGTGGCTGTGGTGAATGACACAGTTGGGAC
  CATGATGACCTGTGGTTATGATGACCACAACTGTGAGATTGGTCTCATTGTGGGCACGGG
  CAGCAACGCCTGCTACATGGAAGAGATGCGCCACATCGACATGGTGGAAGGCGATGAGGG
  GCGGATGTGTATCAATATGGAGTGGGGGGCCTTCGGGGACGATGGCTCGCTCAACGACAT
  TCGCACTGAGTTTGACCAGGAGATTGACATGGGCTCACTGAACCCGGGAAAGCAACTGTT
  TGAGAAGATGATCAGTGGGATGTACATGGGGGAGCTGGTGAGGCTTATCCTGGTGAAGAT
  GGCCAAGGAGGAGCTGCTCTTTGGGGGGAAGCTCAGCCCAGAGCTTCTCAACACCGGTCG
  CTTTGAGACCAAAGACATCTCAGACATTGAAGGGGAGAAGGATGGCATCCGGAAGGCCCG
  TGAGGTCCTGATGCGGTTGGGCCTGGACCCGACTCAGGAGGACTGCGTGGCCACTCACCG
  GATCTGCCAGATCGTGTCCACACGCTCCGCCAGCCTGTGCGCAGCCACCCTGGCCGCCGT
  GCTGCAGCGCATCAAGGAGAACAAAGGCGAGGAGCGGCTGCGCTCTACTATTGGGGTCGA
  CGGTTCCGTCTACAAGAAACACCCCCATTTTGCCAAGCGTCTACATAAGACCGTGCGGCG
  GCTGGTGCCCGGCTGCGATGTCCGCTTCCTCCGCTCCGAGGATGGCAGTGGCAAAGGTGC
  AGCCATGGTGACAGCAGTGGCTTACCGGCTGGCCGATCAACACCGTGCCCGCCAGAAGAC
  ATTAGAGCATCTGCAGCTGAGCCATGACCAGCTGCTGGAGGTCAAGAGGAGGATGAAGGT
  AGAAATGGAGCGAGGTCTGAGCAAGGAGACTCATGCCAGTGCCCCCGTCAAGATGCTGCC
  CACCTACGTGTGTGCTACCCCGGACGGCACAGAGAAAGGGGACTTCTTGGCCTTGGACCT
  TGGAGGAACAAATTTCCGGGTCCTGCTGGTCCGTGTTCGGAATGGGAAGTGGGGTGGAGT
  GGAGATGCACAACAAGATCTACGCCATCCCGCAGGAGGTCATGCACGGCACCGGGGACGA
  GCTCTTTGACCACATTGTCCAGTGCATCGCGGACTTCCTCGAGTACATGGGCATGAAGGG
  CGTGTCCCTGCCTCTGGGTTTTACCTTCTCCTTCCCCTGCCAGCAGAACAGCCTGGACGA
  GAGCATCCTCCTCAAGTGGACAAAAGGCTTCAAGGCATCTGGCTGCGAGGGCGAGGACGT
  GGTGACCCTGCTGAAGGAAGCGATCCACCGGCGAGAGGAGTTTGACCTGGATGTGGTTGC
  TGTGGTGAACGACACAGTCGGAACTATGATGACCTGTGGCTTTGAAGACCCTCACTGTGA
  AGTTGGCCTCATTGTTGGCACGGGCAGCAATGCCTGCTACATGGAGGAGATGCGCAACGT
  GGAACTGGTGGAAGGAGAAGAGGGGCGGATGTGTGTGAACATGGAATGGGGGGCCTTCGG
  GGACAATGGATGCCTAGATGACTTCCGCACAGAATTTGATGTGGCTGTGGATGAGCTTTC
  ACTCAACCCCGGCAAGCAGAGGTTCGAGAAAATGATCAGTGGAATGTACCTGGGTGAGAT
  TGTCCGTAACATTCTCATCGATTTCACCAAGCGTGGACTACTCTTCCGAGGCCGCATCTC
  AGAGCGGCTCAAGACAAGGGGCATCTTTGAAACCAAGTTCTTGTCTCAGATTGAGAGTGA
  CTGCCTGGCCCTGCTGCAAGTCCGAGCCATCCTGCAACACTTAGGGCTTGAGAGCACCTG
  TGACGACAGCATCATTGTTAAGGAGGTGTGCACTGTGGTGGCCCGGCGGGCAGCCCAGCT
  CTGTGGCGCAGGCATGGCCGCTGTGGTGGACAGGATACGAGAAAACCGTGGGCTGGACGC
  TCTCAAAGTGACAGTGGGTGTGGATGGGACCCTCTACAAGCTACATCCTCACTTTGCCAA
  AGTCATGCATGAGACAGTGAAGGACCTGGCTCCGAAATGTGATGTGTCTTTCCTGCAGTC
  AGAGGATGGCAGCGGGAAGGGGGCGGCGCTCATCACTGCTGTGGCCTGCCGCATCCGTGA
  GGCTGGACAGCGATAGAACCCCTGAAATCGGAAGGGACTTCCTCTTTCTCTCCTTCTTCC
  CTGTTTTAAATTATAAGATGTCATCCCCTTGTGTCAGAGACAGACCCCTTGGCTTTTGCT
  TGGCAGAGAGGACCCCACTGGACTGGGTTTTGTCTCTGCATCTCATTGTAGAGCTTGGTG
  GCTGAGCTTGGCCCTATTAAGATAAATAGAGTTCCAAATAAGGATTTGTTCACATGCATC
  ATAACCATTCCCATTGGTTCTCCTAAAACATGAAAATTATCTCCCTTAGTAATCCCCCTT
  GCCAAATTCCATGTCCCTGTATAATTCTACAGGATGGGGACACTAATGAAGATACGGTTG
  CTTCACCTTGGAGCCTGAACATGACATTTCTAAGTGGGGTGCATCCCCCAGCACTGATGT
  TGTTACTGATTCTCCTGTCAGAGATCTGGGAGGTCTCCACTGAGGATGTGAGCCTGATTA
  TCCTATAGGCAGACGTGGGGAGGGTGGAGGGGTGACAGTGGAGGAAAATCCATGGATATC
  CACGCAGCAGCCCCTCTTTAACCTCATCTACAAGCATTTGCCCTGTGGATTCCAGCATTT
  GCCATTCCTGGAATCAAGGAATCCTGAGTCTGGGCAATGAAACCAAAGCCAGGAGTTGAC
  GCATCCTGCAGTTGGGCCAGCTGTCGCATCTCAGCGGGGCGCACATGTTATCCACAAGCA
  ATGGACCTTTGGGGAAGGGGGAGTTTTTAGTTTGTTTTACAAATTTTTCCTGCAAAAGTG
  GAATCACTGTATTTTCATTTTAATTTATATTTGAAATTTTATTTAGTTCTTGAGCATCTG
  CTTCTTCATCTTGACATGTAATGAATGGTCAGTTGTACGTAATGTATTTATATGTTAATT
  TGTTATGTATATAGATGTGCAAGTCTTGTCAGAATTGGCCTCAGTGTAGTTAAAGGGCAG
  AAGGGGAAGATACTGACTAGTCATAGAAATACCTCATTCGCCTGTGGGAAGAGAAGGGAA
  GCCTCTTCAGGGTGAGTGAATGGCAAAGCGGTTGCTTCTGGCTCCTCCTTCCCCTGTGGT
  CTTGGAAGTGTGTGGAAGGCAGGGACAGAGATGGAGGCCGAGCCAATAGACTGAAGAGAC
  CACAGCAATTGGCTCCTCCATCTAGAGATTTTCTTGGCAGTATTCCATGGGATGTTAAGC
  AAAGGAAACCAAAGGAATCGTTTCAAATGGACTCATGGCTTAGAAATCTTTATTCTTAGG
  GCAGTCAGTAGTATTCTAAAGCTTTCTGACAAGATAAAGGAAGTCACCAAAATTTCTTTT
  TTTAAATTGTATCTAATCCTCAACAACAAACCAAAACAGAACAATTAAACAGCCAAATAA
  AACCTCAGGGACAACATTTTTGGTGTATTTGAGCCCTCCCAGCAAGTTTCACCTTGGGTT
  TGTATTTTAAATGTTTTACAAGAATTGTCCATGTGCTTCCCTAGGCTGAGCTGGCATTGG
  TCTGCTGACCTGTTTTTGTGTTTTTCTTTTTTTTATACACAACATTTATTTCAAACTATT
  GGGAGGGATGAGAGTGGCTTAAAAACTTCCATCCCTACTTTTCAAGAGTGCAGTTGATTC
  TGAATCTGAAAGCCCGCCTCTGTCCTAAAATACAAACAAGCACAGACATTAAACCTGGAT
  ACTATATGATAAAGAGGGATGTAACTATTGAATTGGATACAAGGATCAGAATGGAAAGAA
  ACTCACGATGAAATTGAACCTGGTTTTTGTATATTTATCAAACTTGTGCTGAGAATAGTG
  TCTGATTATACGACTTTTAAGCAAAGTTGGGTGTAATTAGGTGAAAACAGCCCAGGTCCT
  CCCGGGAGCACAGAGGGGCTAGGGGCTGGTCCTTCTCGTTTGCTCTAGTCTTGCTTTGCT
  GTCTGGTGTAGCTCCTCTGCTGCTCCCATCTGCACTAATTGACCCAAAACGTGGGTATTT
  CCTGCTACACAAAAGCCAAAAGGTTTCATGTAGATTTTAGTTCACTAAAGGGTGCCCACA
  AAATAGAGATTAATTTTAACTTAAATTTTAAGCTTGAAGATTAGGTACTATCTGTGAAGT
  TACACTTTTTTTTTTTTTTTTAAAGGTAGAGATGTGTGTGTGTGTAGGTATTAAAGATGT
  GTTGTTGGTTTCCAAAAAGGAACACTGGAAAATAAATTTTGAATGTTTATGTTCTCAGAA
  TCAGGTTGACAGTCCCTTGCTGACATGGCTTTGCTTTGTGTAAATACAGTGGATCTCAAT
  CTTCGGGGTGTGATGAATAGCGAATCATCTCAAATCCTTGAGCACTCAGTCTAGTGAAGA
  TGTTGTCATTATGTACAATACATAACTAGTTTAATTAACTATGTGATGTTAACTATTATT
  AATAAATTTTAACATTTTCCAAAATAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000189 unedited
  GTTCGGATTTGNTATACGACTTACTATAGGGCGGCCGCAATCTAGAGTCGACAAGCTTGA
  TATCGGTACCGGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGAAACTCCGGC
  GCAGGAGTCCCGGGCTGCCGCTGGCAACATCGTGTCACCCAGCTAAGAAAATCCGCGGGC
  CCGAGCCACGCGCCTGTGAATCGGAGAGGTCCCACTGCCCGAGTGGAGCCGGGCTGAGAT
  TCTTCTCAAGTTGAGCCTCAGTGATCCTGTGGCCGAAGTTAGCGCCTTGACGTGGGACAA
  CCGGACACGTCGCCAGGACAGAACTGAGGCGCCTTCTAGCAGTTGTGACGCCAAAATCAC
  GTCTCCGGAGACCCGCGCCCTCCGCCAGCCGGGCGCACCCTCGCCGGTAGCCTTCTTTGT
  GCGCCGTCCGGACTCCCAGCTCCCGGCCCGGCAGCCGAGCCCCAGCACAAAGCAGTCGGA
  CCGCGCCGCCCGCCTCCCCTCTCGCGTCTCCGCCTCGGTTTCCCAACTCTGCGCCGTCGG
  GCCGCGGCAGGATGATTGCCTCGCATCTGCTTGCCTACTTCTTCACGGAGCTCAACCATG
  ACCAAGTGCAGAAGGTTGACCAGTATCTCTACCACATGCGCCTCTCTGATGAGACCCTCT
  TGGAGATCTCTAAGCGGTTCCGCAAGGAGATGGGAGAAAGGGCTTGGAGCCACCACTCAC
  CCTACTGCAGCAGTGAAGATGCTGCCCACCTTTGTGAGGTCCACTCCAGATGGGACAGAA
  CACGGAGAGTTCCTTGGCTCTGGATCTTGGAGGGACCCACTCCCGTGTGCTTTGGNTGAA
  AAGTACGGACAATGGGCTCCAAAAGTGG
  
• Restriction Sites: Please inquire
• ACCN: NM_000189
• Insert Size: 5600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000189.4, NP_000180.2
• RefSeq Size: 7109 bp
• RefSeq ORF: 2754 bp
• Locus ID: 3099
• Cytogenetics: 2p12
• Domains: hexokinase
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Metabolic pathways, Starch and sucrose metabolism, Type II diabetes mellitus
• Gene Summary: 'Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]'