PRPK (TP53RK) (NM_033550) Human Tagged ORF Clone
CAT#: RC209748
TP53RK (Myc-DDK-tagged)-Human TP53 regulating kinase (TP53RK)
"NM_033550" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | TP53RK |
Synonyms | BUD32; C20orf64; dJ101A2; GAMOS4; Nori-2; Nori-2p; PRPK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC209748 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCCAGAGCTACTACGCCGGCCGATGGCGAGGAGCCCGCCCCGGAGGCTGAGGCTCTGGCCG CAGCCCGGGAGCGGAGCAGCCGCTTCTTGAGCGGCCTGGAGCTGGTGAAGCAGGGTGCCGAGGCGCGCGT GTTCCGTGGCCACTTCCAGGGCCGCGCGGCGGTGATCAAGCACCGCTTCCCCAAGGGCTACCGGCACCCG GCGCTGGAGGCGCGGCTTGGCAGACGGCGGACGGTGCAGGAGGCCCGGGCGCTCCTCCGCTGTCGCCGCG CTGGAATATCTGCCCCAGTTGTCTTTTTTGTGGACTATGCTTCCAACTGCTTATATATGGAAGAAATTGA AGGCTCAGTGACTGTTCGAGATTATATTCAGTCCACTATGGAGACTGAAAAAACTCCCCAGGGTCTCTCC AACTTAGCCAAGACAATTGGGCAGGTTTTGGCTCGAATGCACGATGAAGACCTCATTCATGGTGATCTCA CCACCTCCAACATGCTCCTGAAACCCCCCCTGGAACAGCTGAACATTGTGCTCATAGACTTTGGGCTGAG TTTCATTTCAGCACTTCCAGAGGATAAGGGAGTAGACCTCTATGTCCTGGAGAAGGCCTTCCTCAGTACC CATCCCAACACTGAAACTGTGTTTGAAGCCTTTCTGAAGAGCTACTCCACCTCCTCCAAAAAGGCCAGGC CAGTGCTAAAAAAATTAGATGAAGTGCGCCTGAGAGGAAGAAAGAGGTCCATGGTTGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209748 protein sequence
Red=Cloning site Green=Tags(s) MAAARATTPADGEEPAPEAEALAAARERSSRFLSGLELVKQGAEARVFRGHFQGRAAVIKHRFPKGYRHP ALEARLGRRRTVQEARALLRCRRAGISAPVVFFVDYASNCLYMEEIEGSVTVRDYIQSTMETEKTPQGLS NLAKTIGQVLARMHDEDLIHGDLTTSNMLLKPPLEQLNIVLIDFGLSFISALPEDKGVDLYVLEKAFLST HPNTETVFEAFLKSYSTSSKKARPVLKKLDEVRLRGRKRSMVG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_033550 |
ORF Size | 759 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_033550.1, NM_033550.2, NM_033550.3, NP_291028.3 |
RefSeq Size | 3384 bp |
RefSeq ORF | 762 bp |
Locus ID | 112858 |
Domains | S_TKc, KOW |
Protein Families | Druggable Genome, Protein Kinase |
MW | 28.1 kDa |
Gene Summary | Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation. [UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC120376 | TP53RK (untagged)-Human TP53 regulating kinase (TP53RK) |
USD 310.00 |
|
SC323584 | TP53RK (untagged)-Kinase deficient mutant (D162A) of Human TP53 regulating kinase (TP53RK) |
USD 420.00 |
|
RG209748 | TP53RK (GFP-tagged) - Human TP53 regulating kinase (TP53RK) |
USD 460.00 |
|
RC209748L1 | Lenti ORF clone of Human TP53 regulating kinase (TP53RK), Myc-DDK-tagged |
USD 768.00 |
|
RC209748L2 | Lenti ORF clone of Human TP53 regulating kinase (TP53RK), mGFP tagged |
USD 620.00 |
|
RC209748L3 | Lenti ORF clone of Human TP53 regulating kinase (TP53RK), Myc-DDK-tagged |
USD 768.00 |
|
RC209748L4 | Lenti ORF clone of Human TP53 regulating kinase (TP53RK), mGFP tagged |
USD 620.00 |
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