PRPK (TP53RK) (NM_033550) Human Tagged ORF Clone

CAT#: RC209748L4

Lenti ORF clone of Human TP53 regulating kinase (TP53RK), mGFP tagged

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: TP53RK
• Synonyms: BUD32; C20orf64; dJ101A2; GAMOS4; Nori-2; Nori-2p; PRPK
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC209748).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_033550
• ORF Size: 759 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_033550.3, NP_291028.3
• RefSeq Size: 3384
• RefSeq ORF: 762
• Locus ID: 112858
• Domains: S_TKc, KOW
• Protein Families: Druggable Genome, Protein Kinase
• MW: 28.1 kDa
• Gene Summary: Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation. [UniProtKB/Swiss-Prot Function]