ZP4 (NM_021186) Human Tagged ORF Clone

CAT#: RC210177L4

Lenti-ORF clone of ZP4 (mGFP-tagged)-Human zona pellucida glycoprotein 4 (ZP4)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: ZP4
• Synonyms: ZBP; Zp-4; ZP1; ZPB
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC210177).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_021186
• ORF Size: 1620 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021186.3, NP_067009.1
• RefSeq Size: 2474
• RefSeq ORF: 1623
• Locus ID: 57829
• Protein Families: Secreted Protein, Transmembrane
• MW: 59.4 kDa
• Gene Summary: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4. [provided by RefSeq, Jul 2008]