ZP4 (NM_021186) Human Tagged ORF Clone

CAT#: RG210177

ZP4 (GFP-tagged) - Human zona pellucida glycoprotein 4 (ZP4)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZP4
• Synonyms: ZBP; Zp-4; ZP1; ZPB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210177 representing NM_021186
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTGCTGCGGTGCGTTTTGCTGTGTGTTTCATTATCTCTTGCTGTGAGTGGCCAGCATAAGCCTG
  AGGCACCAGATTATTCCAGTGTGCTCCACTGTGGGCCGTGGAGCTTCCAGTTTGCTGTAAACCTCAACCA
  GGAGGCAACGTCTCCTCCTGTACTAATAGCTTGGGACAACCAAGGGCTGCTGCACGAGCTGCAGAATGAC
  TCCGACTGTGGCACCTGGATAAGAAAAGGTCCAGGCAGCTCCGTGGTGTTGGAGGCAACCTATAGCAGCT
  GCTATGTCACTGAGTGGGACTCCCACTACATCATGCCAGTTGGAGTTGAAGGAGCAGGCGCGGCTGAACA
  CAAGGTGGTTACAGAGAGGAAGCTGCTCAAGTGTCCTATGGATCTTCTAGCCCGAGATGCTCCAGATACT
  GACTGGTGTGACTCCATCCCAGCACGGGACAGACTGCCATGTGCACCTTCACCCATCTCTCGAGGAGACT
  GTGAAGGGCTAGGCTGTTGTTATAGCTCTGAAGAGGTGAATTCCTGCTACTATGGAAACACTGTGACCTT
  GCATTGTACCCGAGAGGGCCATTTCTCTATTGCTGTGTCTCGGAACGTGACCTCGCCACCACTGCTCTTG
  GATTCTGTGCGCTTGGCCCTTAGGAATGACAGTGCGTGTAACCCTGTGATGGCAACACAAGCTTTTGTTC
  TGTTCCAGTTTCCATTTACTTCCTGTGGCACCACAAGACAGATCACTGGAGACCGAGCAGTATATGAAAA
  TGAACTGGTGGCAACTAGGGATGTGAAAAATGGGAGCCGTGGCTCTGTCACTCGTGACAGCATCTTCAGG
  CTCCATGTCAGCTGCAGCTACTCAGTAAGTAGCAACTCTCTCCCAATCAATGTCCAGGTTTTCACTCTCC
  CACCACCCTTTCCTGAGACCCAGCCTGGACCCCTCACTCTGGAACTTCAGATTGCCAAAGATAAAAACTA
  TGGCTCTTACTACGGTGTTGGTGACTACCCAGTGGTGAAGTTGCTTCGGGATCCCATTTACGTGGAGGTC
  TCCATCCTTCACAGAACAGACCCCTACCTGGGGCTGCTCCTACAACAGTGTTGGGCAACACCCAGCACTG
  ACCCCCTGAGTCAGCCACAGTGGCCCATCCTGGTAAAGGGCTGCCCCTACATTGGAGACAACTATCAGAC
  CCAGCTGATCCCTGTCCAGAAAGCCTTGGATCTTCCATTTCCCTCTCACCACCAGCGCTTCAGCATCTTC
  ACCTTCAGCTTTGTGAACCCTACAGTGGAGAAACAGGCCCTCAGGGGACCGGTGCATCTGCACTGCAGCG
  TGTCAGTCTGCCAGCCTGCTGAGACACCATCCTGTGTGGTGACCTGTCCTGATCTCAGTCGAAGAAGAAA
  TTTTGACAACAGTTCTCAGAACACTACTGCTAGTGTTTCTAGCAAAGGCCCCATGATTCTACTCCAAGCC
  ACTAAGGACCCTCCAGAAAAGCTCCGTGTTCCTGTAGACTCGAAAGTTCTGTGGGTGGCAGGCCTTTCTG
  GGACCTTAATCCTTGGAGCCTTGTTAGTATCCTACTTGGCTGTCAAGAAACAGAAGAGTTGCCCAGACCA
  AATGTGTCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210177 representing NM_021186
  Red=Cloning site Green=Tags(s)
  
  MWLLRCVLLCVSLSLAVSGQHKPEAPDYSSVLHCGPWSFQFAVNLNQEATSPPVLIAWDNQGLLHELQND
  SDCGTWIRKGPGSSVVLEATYSSCYVTEWDSHYIMPVGVEGAGAAEHKVVTERKLLKCPMDLLARDAPDT
  DWCDSIPARDRLPCAPSPISRGDCEGLGCCYSSEEVNSCYYGNTVTLHCTREGHFSIAVSRNVTSPPLLL
  DSVRLALRNDSACNPVMATQAFVLFQFPFTSCGTTRQITGDRAVYENELVATRDVKNGSRGSVTRDSIFR
  LHVSCSYSVSSNSLPINVQVFTLPPPFPETQPGPLTLELQIAKDKNYGSYYGVGDYPVVKLLRDPIYVEV
  SILHRTDPYLGLLLQQCWATPSTDPLSQPQWPILVKGCPYIGDNYQTQLIPVQKALDLPFPSHHQRFSIF
  TFSFVNPTVEKQALRGPVHLHCSVSVCQPAETPSCVVTCPDLSRRRNFDNSSQNTTASVSSKGPMILLQA
  TKDPPEKLRVPVDSKVLWVAGLSGTLILGALLVSYLAVKKQKSCPDQMCQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021186
• ORF Size: 1620 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021186.3, NP_067009.1
• RefSeq Size: 2474
• RefSeq ORF: 1623
• Locus ID: 57829
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4. [provided by RefSeq, Jul 2008]