CENTD1 (ARAP2) (NM_015230) Human Tagged ORF Clone
CAT#: RC216798
- TrueORF®
ARAP2 (Myc-DDK-tagged)-Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2)
"NM_015230" in other vectors (2)
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Other products for "ARAP2"
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ARAP2 |
Synonyms | CENTD1; PARX |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC216798 representing NM_015230
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTCAGTCAGTGAAGTAAATGTGGATATAAAAGATTTCCTAATGAGCATTAATTTGGAGCAGTATC TCTTACATTTCCATGAGTCTGGTTTTACTACTGTGAAGGACTGTGCAGCAATAAATGACAGCCTGCTGCA GAAAATTGGAATATCACCTACAGGTCACCGTAGGAGGATACTTAAACAGTTACAGATAATCTTGTCAAAA ATGCAAGATATTCCAATATATGCAAATGTTCATAAAACTAAGAAGAATGATGACCCTTCAAAGGATTACC ATGTTCCATCTTCTGATCAGAATATCTGCATAGAACTTTCCAATTCTGGTAGTGTTCAGACATCTAGCCC ACCGCAGTTGGAGACTGTTAGAAAAAATCTTGAAGACAGTGATGCAAGTGTGGAAAGAAGCCAGTATCCT CAATCAGATGATAAGCTGTCTCCTCCTAAACGCGACTTCCCCACTGCAGAGGAACCACACCTGAATTTGG GTTCTTTGAATGATTCTTTATTTGGTAGTGACAATATTAAAATAGAATCATTGATTACAAAGAAGACTGT GGATCACACAGTTGAAGAACAACAAACAGAAAAAGTTAAATTGATCACAGAAAATCTCAGTAAGCTCCCT AATGCAGACTCTGAATGCCTTTCTTTTGTTGGCTGTTCAACATCAGGAACAAATTCTGGAAATGGAACAA ATGGTTTATTAGAAGGATCACCACCATCCCCATTCTTTAAGTTTCAAGGAGAAATGATTGTAAATGACTT GTATGTTCCATCATCACCAATCCTAGCACCTGTGAGAAGTCGTAGCAAGTTGGTTTCAAGACCATCTCGA TCTTTTCTGCTAAGACATCGACCTGTACCAGAGATTCCAGGGTCAACAAAAGGAGTTTCTGGGAGCTATT TCCGTGAAAGAAGAAATGTTGCTACCTCAACTGAAAAATCTGTGGCATGGCAAAATTCAAATGAGGAGAA TTCATCTTCCATCTTTCCTTATGGAGAGACCTTTCTCTTCCAGAGACTAGAAAATTCCAAGAAGCGATCT ATAAAGAATGAATTTTTGACCCAGGGAGAAGCACTCAAAGGGGAAGCAGCTACTGCAACAAACTCTTTTA TCATCAAATCAAGCATATACGATAACAGAAAGGAGAAAATAAGCGAGGACAAGGTGGAAGATATTTGGAT ACCTCGAGAGGACAAAAACAATTTTTTGATAGACACTGCTTCTGAATCAGAATACTCAACAGTAGAAGAA TGCTTTCAGAGTTTAAGAAGAAAAAATTCAAAGGCATCTAAATCTAGGACTCAAAAAGCCTTGATTTTGG ACTCCGTTAATAGGCACAGTTATCCGTTAAGCTCAACAAGTGGAAATGCTGATTCATCAGCCGTTTCTTC ACAGGCAATATCTCCCTATGCCTGCTTTTATGGAGCATCTGCAAAGAAGGTTAAATCAGGATGGCTGGAT AAACTCTCTCCTCAAGGAAAACGCATGTTTCAAAAGAGATGGGTGAAATTTGATGGCCTTAGCATTTCTT ACTACAATAATGAGAAGGAGATGTATTCGAAAGGAATAATTCCCCTTTCTGCTATATCAACAGTACGAGT TCAAGGAGACAACAAATTTGAAGTTGTTACAACACAAAGAACTTTTGTTTTTAGAGTAGAAAAAGAAGAG GAGAGAAATGACTGGATCAGCATACTATTAAATGCACTGAAATCACAATCCCTTACCTCGCAGTCTCAAG CTGTTGTTACACCTGAGAAATGTGGATATCTTGAATTGAGAGGCTATAAGGCAAAAATTTTTACTGTGTT AAGTGGAAACAGTGTGTGGCTTTGCAAAAACGAACAGGATTTTAAGAGTGGACTTGGTATTACCATAATT CCTATGAATGTAGCAAATGTAAAGCAAGTGGACCGAACTGTGAAACAATCTTTTGAAATAATCACTCCCT ACAGGAGTTTCAGCTTTACAGCCGAGACTGAAAAGGAGAAACAAGACTGGATTGAAGCTGTGCAGCAATC AATAGCAGAAACTCTCTCTGATTATGAAGTAGCTGAGAAGATTTGGTTCAATGAATCCAACAGGAGCTGT GCAGATTGTAAAGCCCCAGATCCTGACTGGGCATCCATCAATCTCTGTGTTGTCATCTGTAAGAAGTGTG CAGGACAGCATAGATCTTTAGGACCAAAAGATTCCAAGGTTAGAAGTCTAAAAATGGATGCTAGCATTTG GAGCAATGAACTCATCGAGCTTTTTATTGTCATTGGAAACAAAAGAGCAAATGACTTTTGGGCTGGTAAT CTTCAAAAGGATGAAGAATTACATATGGACTCACCAGTAGAAAAGAGAAAAAACTTTATTACTCAGAAAT ATAAAGAAGGAAAATTCAGAAAAACTCTTTTGGCATCTCTCACCAAAGAAGAATTAAATAAGGCTCTATG TGCTGCTGTAGTGAAACCGGATGTTCTAGAAACAATGGCTTTGCTGTTCAGTGGAGCAGATGTCATGTGT GCCACCGGAGACCCCGTGCATAGCACCCCCTATCTGCTAGCCAAGAAAGCTGGGCAAAGTCTGCAAATGG AATTTCTCTACCATAACAAATTCTCAGATTTCCCTCAACATGATATTCATTCCGAGGGTGTATTAAGTCA AGAGTCTTCCCAGTCCACATTCCTCTGTGACTTTTTATATCAAGCTCCTTCTGCTGCTTCTAAACTCTCT TCAGAGAAAAAACTGCTTGAAGAGACAAATAAAAAATGGTGTGTTTTGGAAGGAGGCTTCTTGAGTTACT ATGAAAATGATAAGTCTACCACACCTAATGACACCATTAATATCAATGAAGTTATCTGCCTGGCTATACA CAAAGAGGACTTCTATTTAAATACTGGGCCCATCTTTATCTTTGAGATCTACTTACCCTCCGAACGTGTG TTTTTATTTGGAGCTGAAACATCTCAAGCTCAAAGAAAATGGACAGAGGCAATAGCCAAGCATTTTGTTC CCTTATTTGCTGAAAACTTAACAGAAGCTGACTATGATTTGATTGGTCAACTCTTCTACAAAGACTGCCA TGCCCTGGATCAGTGGAGAAAAGGCTGGTTTGCTATGGACAAATCCAGCTTGCATTTTTGCCTTCAAATG CAAGAAGTTCAGGGAGATAGAATGCACTTAAGAAGACTGCAAGAGCTAACAATCAGCACAATGGTTCAAA ATGGGGAAAAACTGGATGTTTTACTCTTGGTAGAAAAAGGGAGAACATTATACATCCATGGGCATACCAA GTTGGATTTCACAGTCTGGCATACTGCAATTGAAAAAGCAGCAGGTACAGATGGTAATGCTTTACAAGAT CAGCAGCTCAGCAAAAATGACGTTCCCATTATAGTGAACAGCTGTATAGCATTTGTTACACAGTATGGTT TAGGATGCAAATATATCTATCAAAAGAATGGTGATCCTTTGCATATAAGTGAACTCCTGGAGAGTTTCAA AAAGGATGCAAGAAGCTTTAAATTGAGGGCTGGAAAACATCAGCTTGAAGATGTGACGGCTGTGTTGAAA AGTTTTCTCTCTGACATTGATGATGCACTGCTTACTAAGGAGCTCTACCCATATTGGATCTCTGCTTTAG ATACGCAAGATGACAAGGAAAGAATTAAAAAATATGGAGCATTTATACGTTCTCTTCCAGGGGTCAACCG AGCAACACTAGCAGCTATCATTGAACACCTGTATAGGGTTCAGAAATGCTCAGAAATCAATCACATGAAT GCCCATAATTTGGCCTTGGTCTTTTCATCCTGTTTGTTTCAAACGAAGGGACAAACTAGTGAAGAAGTGA ATGTAATTGAGGACCTAATTAATAATTATGTAGAAATATTTGAGGTTAAAGAAGATCAAGTCAAACAAAT GGACATAGAAAATAGCTTTATTACCAAGTGGAAAGACACCCAAGTTTCCCAGGCTGGAGATTTGTTAATT GAAGTATATGTAGAAAGGAAGGAACCCGACTGTAGTATTATAATTCGGATATCTCCTGTGATGGAAGCAG AAGAATTAACTAATGATATATTAGCGATAAAAAATATTATTCCTACAAAAGGTGATATTTGGGCCACATT TGAAGTCATTGAAAATGAAGAGCTAGAGCGTCCTCTTCACTACAAGGAAAATGTACTGGAGCAGGTGCTT CGGTGGAGTTCATTAGCTGAACCTGGCTCTGCTTACCTGGTGGTGAAGAGATTCTTAACCGCTGACACAA TTAAACACTGCAGTGACCGGAGTACACTGGGAAGCATCAAAGAAGGAATCTTGAAAATCAAAGAAGAACC ATCCAAAATACTATCTGGAAATAAGTTTCAAGACCGGTATTTTGTTTTACGAGATGGGTTTCTCTTTCTT TACAAGGATGTGAAGAGTAGTAAACATGACAAGATGTTTTCTCTCAGTTCCATGAAGTTTTATCGTGGAG TGAAAAAGAAAATGAAGCCTCCAACAAGCTGGGGATTGACCGCATATTCTGAGAAACATCACTGGCACCT GTGTTGTGATAGTTCACAAACTCAGACGGAGTGGATGACCAGTATCTTTATTGCCCAGCATGAATATGAT ATATGGCCACCAGCTGGAAAGGAACGAAAACGTTCAATAACCAAAAATCCCAAAATTGGAGGTTTGCCTC TGATTCCTATACAGCATGAGGGGAATGCAACCTTGGCCCGGAAAAATATTGAGAGTGCAAGAGCAGAACT TGAAAGGCTGCGGCTCAGTGAAAAGTGTGATAAAGAGTCCGTGGACTCTAGCTTAAAGGAGAGAGCTTCC ATGGTGGCCCACTGCCTGGAGCACAAGGACGATAAACTTCGAAATCGACCCCGAAAACATCGGAGTTTCA ACTGCCTGGAGGACACAGAGCCTGAAGCCCCACTTGGGCAACCAAAAGGCCATAAAGGCCTAAAGACATT GAGGAAGACTGAGGACAGGAATAGCAAAGCTACTTTGGACTCTGATCATAAGTTACCATCAAGGGTAATT GAAGAACTTAATGTGGTTCTACAACGGTCAAGAACCCTTCCAAAAGAATTACAGGATGAGCAGATTTTGA AG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216798 representing NM_015230
Red=Cloning site Green=Tags(s) MSSVSEVNVDIKDFLMSINLEQYLLHFHESGFTTVKDCAAINDSLLQKIGISPTGHRRRILKQLQIILSK MQDIPIYANVHKTKKNDDPSKDYHVPSSDQNICIELSNSGSVQTSSPPQLETVRKNLEDSDASVERSQYP QSDDKLSPPKRDFPTAEEPHLNLGSLNDSLFGSDNIKIESLITKKTVDHTVEEQQTEKVKLITENLSKLP NADSECLSFVGCSTSGTNSGNGTNGLLEGSPPSPFFKFQGEMIVNDLYVPSSPILAPVRSRSKLVSRPSR SFLLRHRPVPEIPGSTKGVSGSYFRERRNVATSTEKSVAWQNSNEENSSSIFPYGETFLFQRLENSKKRS IKNEFLTQGEALKGEAATATNSFIIKSSIYDNRKEKISEDKVEDIWIPREDKNNFLIDTASESEYSTVEE CFQSLRRKNSKASKSRTQKALILDSVNRHSYPLSSTSGNADSSAVSSQAISPYACFYGASAKKVKSGWLD KLSPQGKRMFQKRWVKFDGLSISYYNNEKEMYSKGIIPLSAISTVRVQGDNKFEVVTTQRTFVFRVEKEE ERNDWISILLNALKSQSLTSQSQAVVTPEKCGYLELRGYKAKIFTVLSGNSVWLCKNEQDFKSGLGITII PMNVANVKQVDRTVKQSFEIITPYRSFSFTAETEKEKQDWIEAVQQSIAETLSDYEVAEKIWFNESNRSC ADCKAPDPDWASINLCVVICKKCAGQHRSLGPKDSKVRSLKMDASIWSNELIELFIVIGNKRANDFWAGN LQKDEELHMDSPVEKRKNFITQKYKEGKFRKTLLASLTKEELNKALCAAVVKPDVLETMALLFSGADVMC ATGDPVHSTPYLLAKKAGQSLQMEFLYHNKFSDFPQHDIHSEGVLSQESSQSTFLCDFLYQAPSAASKLS SEKKLLEETNKKWCVLEGGFLSYYENDKSTTPNDTININEVICLAIHKEDFYLNTGPIFIFEIYLPSERV FLFGAETSQAQRKWTEAIAKHFVPLFAENLTEADYDLIGQLFYKDCHALDQWRKGWFAMDKSSLHFCLQM QEVQGDRMHLRRLQELTISTMVQNGEKLDVLLLVEKGRTLYIHGHTKLDFTVWHTAIEKAAGTDGNALQD QQLSKNDVPIIVNSCIAFVTQYGLGCKYIYQKNGDPLHISELLESFKKDARSFKLRAGKHQLEDVTAVLK SFLSDIDDALLTKELYPYWISALDTQDDKERIKKYGAFIRSLPGVNRATLAAIIEHLYRVQKCSEINHMN AHNLALVFSSCLFQTKGQTSEEVNVIEDLINNYVEIFEVKEDQVKQMDIENSFITKWKDTQVSQAGDLLI EVYVERKEPDCSIIIRISPVMEAEELTNDILAIKNIIPTKGDIWATFEVIENEELERPLHYKENVLEQVL RWSSLAEPGSAYLVVKRFLTADTIKHCSDRSTLGSIKEGILKIKEEPSKILSGNKFQDRYFVLRDGFLFL YKDVKSSKHDKMFSLSSMKFYRGVKKKMKPPTSWGLTAYSEKHHWHLCCDSSQTQTEWMTSIFIAQHEYD IWPPAGKERKRSITKNPKIGGLPLIPIQHEGNATLARKNIESARAELERLRLSEKCDKESVDSSLKERAS MVAHCLEHKDDKLRNRPRKHRSFNCLEDTEPEAPLGQPKGHKGLKTLRKTEDRNSKATLDSDHKLPSRVI EELNVVLQRSRTLPKELQDEQILK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015230 |
ORF Size | 5112 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015230.1, NM_015230.2, NM_015230.3, NP_056045.2 |
RefSeq Size | 7360 bp |
RefSeq ORF | 5115 bp |
Locus ID | 116984 |
Domains | RhoGAP, ArfGap, SAM, PH |
Protein Pathways | Endocytosis |
MW | 193.3 kDa |
Gene Summary | The protein encoded by this gene contains ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology domains. The protein is a phosphatidylinositol (3,4,5)-trisphosphate-dependent Arf6 GAP that binds RhoA-GTP, but it lacks the predicted catalytic arginine in the RHO-GAP domain and does not have RHO-GAP activity. The protein associates with focal adhesions and functions downstream of RhoA to regulate focal adhesion dynamics. [provided by RefSeq, Sep 2008] |
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