CENTD1 (ARAP2) (NM_015230) Human Tagged ORF Clone

CAT#: RG216798

ARAP2 (GFP-tagged) - Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 (ARAP2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ARAP2
• Synonyms: CENTD1; PARX
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216798 representing NM_015230
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCAGTCAGTGAAGTAAATGTGGATATAAAAGATTTCCTAATGAGCATTAATTTGGAGCAGTATC
  TCTTACATTTCCATGAGTCTGGTTTTACTACTGTGAAGGACTGTGCAGCAATAAATGACAGCCTGCTGCA
  GAAAATTGGAATATCACCTACAGGTCACCGTAGGAGGATACTTAAACAGTTACAGATAATCTTGTCAAAA
  ATGCAAGATATTCCAATATATGCAAATGTTCATAAAACTAAGAAGAATGATGACCCTTCAAAGGATTACC
  ATGTTCCATCTTCTGATCAGAATATCTGCATAGAACTTTCCAATTCTGGTAGTGTTCAGACATCTAGCCC
  ACCGCAGTTGGAGACTGTTAGAAAAAATCTTGAAGACAGTGATGCAAGTGTGGAAAGAAGCCAGTATCCT
  CAATCAGATGATAAGCTGTCTCCTCCTAAACGCGACTTCCCCACTGCAGAGGAACCACACCTGAATTTGG
  GTTCTTTGAATGATTCTTTATTTGGTAGTGACAATATTAAAATAGAATCATTGATTACAAAGAAGACTGT
  GGATCACACAGTTGAAGAACAACAAACAGAAAAAGTTAAATTGATCACAGAAAATCTCAGTAAGCTCCCT
  AATGCAGACTCTGAATGCCTTTCTTTTGTTGGCTGTTCAACATCAGGAACAAATTCTGGAAATGGAACAA
  ATGGTTTATTAGAAGGATCACCACCATCCCCATTCTTTAAGTTTCAAGGAGAAATGATTGTAAATGACTT
  GTATGTTCCATCATCACCAATCCTAGCACCTGTGAGAAGTCGTAGCAAGTTGGTTTCAAGACCATCTCGA
  TCTTTTCTGCTAAGACATCGACCTGTACCAGAGATTCCAGGGTCAACAAAAGGAGTTTCTGGGAGCTATT
  TCCGTGAAAGAAGAAATGTTGCTACCTCAACTGAAAAATCTGTGGCATGGCAAAATTCAAATGAGGAGAA
  TTCATCTTCCATCTTTCCTTATGGAGAGACCTTTCTCTTCCAGAGACTAGAAAATTCCAAGAAGCGATCT
  ATAAAGAATGAATTTTTGACCCAGGGAGAAGCACTCAAAGGGGAAGCAGCTACTGCAACAAACTCTTTTA
  TCATCAAATCAAGCATATACGATAACAGAAAGGAGAAAATAAGCGAGGACAAGGTGGAAGATATTTGGAT
  ACCTCGAGAGGACAAAAACAATTTTTTGATAGACACTGCTTCTGAATCAGAATACTCAACAGTAGAAGAA
  TGCTTTCAGAGTTTAAGAAGAAAAAATTCAAAGGCATCTAAATCTAGGACTCAAAAAGCCTTGATTTTGG
  ACTCCGTTAATAGGCACAGTTATCCGTTAAGCTCAACAAGTGGAAATGCTGATTCATCAGCCGTTTCTTC
  ACAGGCAATATCTCCCTATGCCTGCTTTTATGGAGCATCTGCAAAGAAGGTTAAATCAGGATGGCTGGAT
  AAACTCTCTCCTCAAGGAAAACGCATGTTTCAAAAGAGATGGGTGAAATTTGATGGCCTTAGCATTTCTT
  ACTACAATAATGAGAAGGAGATGTATTCGAAAGGAATAATTCCCCTTTCTGCTATATCAACAGTACGAGT
  TCAAGGAGACAACAAATTTGAAGTTGTTACAACACAAAGAACTTTTGTTTTTAGAGTAGAAAAAGAAGAG
  GAGAGAAATGACTGGATCAGCATACTATTAAATGCACTGAAATCACAATCCCTTACCTCGCAGTCTCAAG
  CTGTTGTTACACCTGAGAAATGTGGATATCTTGAATTGAGAGGCTATAAGGCAAAAATTTTTACTGTGTT
  AAGTGGAAACAGTGTGTGGCTTTGCAAAAACGAACAGGATTTTAAGAGTGGACTTGGTATTACCATAATT
  CCTATGAATGTAGCAAATGTAAAGCAAGTGGACCGAACTGTGAAACAATCTTTTGAAATAATCACTCCCT
  ACAGGAGTTTCAGCTTTACAGCCGAGACTGAAAAGGAGAAACAAGACTGGATTGAAGCTGTGCAGCAATC
  AATAGCAGAAACTCTCTCTGATTATGAAGTAGCTGAGAAGATTTGGTTCAATGAATCCAACAGGAGCTGT
  GCAGATTGTAAAGCCCCAGATCCTGACTGGGCATCCATCAATCTCTGTGTTGTCATCTGTAAGAAGTGTG
  CAGGACAGCATAGATCTTTAGGACCAAAAGATTCCAAGGTTAGAAGTCTAAAAATGGATGCTAGCATTTG
  GAGCAATGAACTCATCGAGCTTTTTATTGTCATTGGAAACAAAAGAGCAAATGACTTTTGGGCTGGTAAT
  CTTCAAAAGGATGAAGAATTACATATGGACTCACCAGTAGAAAAGAGAAAAAACTTTATTACTCAGAAAT
  ATAAAGAAGGAAAATTCAGAAAAACTCTTTTGGCATCTCTCACCAAAGAAGAATTAAATAAGGCTCTATG
  TGCTGCTGTAGTGAAACCGGATGTTCTAGAAACAATGGCTTTGCTGTTCAGTGGAGCAGATGTCATGTGT
  GCCACCGGAGACCCCGTGCATAGCACCCCCTATCTGCTAGCCAAGAAAGCTGGGCAAAGTCTGCAAATGG
  AATTTCTCTACCATAACAAATTCTCAGATTTCCCTCAACATGATATTCATTCCGAGGGTGTATTAAGTCA
  AGAGTCTTCCCAGTCCACATTCCTCTGTGACTTTTTATATCAAGCTCCTTCTGCTGCTTCTAAACTCTCT
  TCAGAGAAAAAACTGCTTGAAGAGACAAATAAAAAATGGTGTGTTTTGGAAGGAGGCTTCTTGAGTTACT
  ATGAAAATGATAAGTCTACCACACCTAATGACACCATTAATATCAATGAAGTTATCTGCCTGGCTATACA
  CAAAGAGGACTTCTATTTAAATACTGGGCCCATCTTTATCTTTGAGATCTACTTACCCTCCGAACGTGTG
  TTTTTATTTGGAGCTGAAACATCTCAAGCTCAAAGAAAATGGACAGAGGCAATAGCCAAGCATTTTGTTC
  CCTTATTTGCTGAAAACTTAACAGAAGCTGACTATGATTTGATTGGTCAACTCTTCTACAAAGACTGCCA
  TGCCCTGGATCAGTGGAGAAAAGGCTGGTTTGCTATGGACAAATCCAGCTTGCATTTTTGCCTTCAAATG
  CAAGAAGTTCAGGGAGATAGAATGCACTTAAGAAGACTGCAAGAGCTAACAATCAGCACAATGGTTCAAA
  ATGGGGAAAAACTGGATGTTTTACTCTTGGTAGAAAAAGGGAGAACATTATACATCCATGGGCATACCAA
  GTTGGATTTCACAGTCTGGCATACTGCAATTGAAAAAGCAGCAGGTACAGATGGTAATGCTTTACAAGAT
  CAGCAGCTCAGCAAAAATGACGTTCCCATTATAGTGAACAGCTGTATAGCATTTGTTACACAGTATGGTT
  TAGGATGCAAATATATCTATCAAAAGAATGGTGATCCTTTGCATATAAGTGAACTCCTGGAGAGTTTCAA
  AAAGGATGCAAGAAGCTTTAAATTGAGGGCTGGAAAACATCAGCTTGAAGATGTGACGGCTGTGTTGAAA
  AGTTTTCTCTCTGACATTGATGATGCACTGCTTACTAAGGAGCTCTACCCATATTGGATCTCTGCTTTAG
  ATACGCAAGATGACAAGGAAAGAATTAAAAAATATGGAGCATTTATACGTTCTCTTCCAGGGGTCAACCG
  AGCAACACTAGCAGCTATCATTGAACACCTGTATAGGGTTCAGAAATGCTCAGAAATCAATCACATGAAT
  GCCCATAATTTGGCCTTGGTCTTTTCATCCTGTTTGTTTCAAACGAAGGGACAAACTAGTGAAGAAGTGA
  ATGTAATTGAGGACCTAATTAATAATTATGTAGAAATATTTGAGGTTAAAGAAGATCAAGTCAAACAAAT
  GGACATAGAAAATAGCTTTATTACCAAGTGGAAAGACACCCAAGTTTCCCAGGCTGGAGATTTGTTAATT
  GAAGTATATGTAGAAAGGAAGGAACCCGACTGTAGTATTATAATTCGGATATCTCCTGTGATGGAAGCAG
  AAGAATTAACTAATGATATATTAGCGATAAAAAATATTATTCCTACAAAAGGTGATATTTGGGCCACATT
  TGAAGTCATTGAAAATGAAGAGCTAGAGCGTCCTCTTCACTACAAGGAAAATGTACTGGAGCAGGTGCTT
  CGGTGGAGTTCATTAGCTGAACCTGGCTCTGCTTACCTGGTGGTGAAGAGATTCTTAACCGCTGACACAA
  TTAAACACTGCAGTGACCGGAGTACACTGGGAAGCATCAAAGAAGGAATCTTGAAAATCAAAGAAGAACC
  ATCCAAAATACTATCTGGAAATAAGTTTCAAGACCGGTATTTTGTTTTACGAGATGGGTTTCTCTTTCTT
  TACAAGGATGTGAAGAGTAGTAAACATGACAAGATGTTTTCTCTCAGTTCCATGAAGTTTTATCGTGGAG
  TGAAAAAGAAAATGAAGCCTCCAACAAGCTGGGGATTGACCGCATATTCTGAGAAACATCACTGGCACCT
  GTGTTGTGATAGTTCACAAACTCAGACGGAGTGGATGACCAGTATCTTTATTGCCCAGCATGAATATGAT
  ATATGGCCACCAGCTGGAAAGGAACGAAAACGTTCAATAACCAAAAATCCCAAAATTGGAGGTTTGCCTC
  TGATTCCTATACAGCATGAGGGGAATGCAACCTTGGCCCGGAAAAATATTGAGAGTGCAAGAGCAGAACT
  TGAAAGGCTGCGGCTCAGTGAAAAGTGTGATAAAGAGTCCGTGGACTCTAGCTTAAAGGAGAGAGCTTCC
  ATGGTGGCCCACTGCCTGGAGCACAAGGACGATAAACTTCGAAATCGACCCCGAAAACATCGGAGTTTCA
  ACTGCCTGGAGGACACAGAGCCTGAAGCCCCACTTGGGCAACCAAAAGGCCATAAAGGCCTAAAGACATT
  GAGGAAGACTGAGGACAGGAATAGCAAAGCTACTTTGGACTCTGATCATAAGTTACCATCAAGGGTAATT
  GAAGAACTTAATGTGGTTCTACAACGGTCAAGAACCCTTCCAAAAGAATTACAGGATGAGCAGATTTTGA
  AG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216798 representing NM_015230
  Red=Cloning site Green=Tags(s)
  
  MSSVSEVNVDIKDFLMSINLEQYLLHFHESGFTTVKDCAAINDSLLQKIGISPTGHRRRILKQLQIILSK
  MQDIPIYANVHKTKKNDDPSKDYHVPSSDQNICIELSNSGSVQTSSPPQLETVRKNLEDSDASVERSQYP
  QSDDKLSPPKRDFPTAEEPHLNLGSLNDSLFGSDNIKIESLITKKTVDHTVEEQQTEKVKLITENLSKLP
  NADSECLSFVGCSTSGTNSGNGTNGLLEGSPPSPFFKFQGEMIVNDLYVPSSPILAPVRSRSKLVSRPSR
  SFLLRHRPVPEIPGSTKGVSGSYFRERRNVATSTEKSVAWQNSNEENSSSIFPYGETFLFQRLENSKKRS
  IKNEFLTQGEALKGEAATATNSFIIKSSIYDNRKEKISEDKVEDIWIPREDKNNFLIDTASESEYSTVEE
  CFQSLRRKNSKASKSRTQKALILDSVNRHSYPLSSTSGNADSSAVSSQAISPYACFYGASAKKVKSGWLD
  KLSPQGKRMFQKRWVKFDGLSISYYNNEKEMYSKGIIPLSAISTVRVQGDNKFEVVTTQRTFVFRVEKEE
  ERNDWISILLNALKSQSLTSQSQAVVTPEKCGYLELRGYKAKIFTVLSGNSVWLCKNEQDFKSGLGITII
  PMNVANVKQVDRTVKQSFEIITPYRSFSFTAETEKEKQDWIEAVQQSIAETLSDYEVAEKIWFNESNRSC
  ADCKAPDPDWASINLCVVICKKCAGQHRSLGPKDSKVRSLKMDASIWSNELIELFIVIGNKRANDFWAGN
  LQKDEELHMDSPVEKRKNFITQKYKEGKFRKTLLASLTKEELNKALCAAVVKPDVLETMALLFSGADVMC
  ATGDPVHSTPYLLAKKAGQSLQMEFLYHNKFSDFPQHDIHSEGVLSQESSQSTFLCDFLYQAPSAASKLS
  SEKKLLEETNKKWCVLEGGFLSYYENDKSTTPNDTININEVICLAIHKEDFYLNTGPIFIFEIYLPSERV
  FLFGAETSQAQRKWTEAIAKHFVPLFAENLTEADYDLIGQLFYKDCHALDQWRKGWFAMDKSSLHFCLQM
  QEVQGDRMHLRRLQELTISTMVQNGEKLDVLLLVEKGRTLYIHGHTKLDFTVWHTAIEKAAGTDGNALQD
  QQLSKNDVPIIVNSCIAFVTQYGLGCKYIYQKNGDPLHISELLESFKKDARSFKLRAGKHQLEDVTAVLK
  SFLSDIDDALLTKELYPYWISALDTQDDKERIKKYGAFIRSLPGVNRATLAAIIEHLYRVQKCSEINHMN
  AHNLALVFSSCLFQTKGQTSEEVNVIEDLINNYVEIFEVKEDQVKQMDIENSFITKWKDTQVSQAGDLLI
  EVYVERKEPDCSIIIRISPVMEAEELTNDILAIKNIIPTKGDIWATFEVIENEELERPLHYKENVLEQVL
  RWSSLAEPGSAYLVVKRFLTADTIKHCSDRSTLGSIKEGILKIKEEPSKILSGNKFQDRYFVLRDGFLFL
  YKDVKSSKHDKMFSLSSMKFYRGVKKKMKPPTSWGLTAYSEKHHWHLCCDSSQTQTEWMTSIFIAQHEYD
  IWPPAGKERKRSITKNPKIGGLPLIPIQHEGNATLARKNIESARAELERLRLSEKCDKESVDSSLKERAS
  MVAHCLEHKDDKLRNRPRKHRSFNCLEDTEPEAPLGQPKGHKGLKTLRKTEDRNSKATLDSDHKLPSRVI
  EELNVVLQRSRTLPKELQDEQILK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015230
• ORF Size: 5112 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015230.2, NP_056045.2
• RefSeq Size: 7360
• RefSeq ORF: 5115
• Locus ID: 116984
• Domains: RhoGAP, ArfGap, SAM, PH
• Protein Pathways: Endocytosis
• Gene Summary: The protein encoded by this gene contains ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology domains. The protein is a phosphatidylinositol (3,4,5)-trisphosphate-dependent Arf6 GAP that binds RhoA-GTP, but it lacks the predicted catalytic arginine in the RHO-GAP domain and does not have RHO-GAP activity. The protein associates with focal adhesions and functions downstream of RhoA to regulate focal adhesion dynamics. [provided by RefSeq, Sep 2008]