Caspr (CNTNAP1) (NM_003632) Human Tagged ORF Clone

CAT#: RC218019

CNTNAP1 (Myc-DDK-tagged)-Human contactin associated protein 1 (CNTNAP1)

Product Images

Western blot validation of overexpression lysate (Cat# LY401200) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218019 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNTNAP1
• Synonyms: CASPR; CHN3; CNTNAP; NRXN4; P190
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218019 representing NM_003632
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCATCTCCGGCTCTTCTGCATCCTGCTCGCCGCGGTCTCAGGAGCCGAGGGCTGGGGCTACTACG
  GCTGCGACGAGGAGCTGGTGGGTCCCCTGTATGCACGCTCCCTGGGCGCCTCCTCCTACTACAGTCTCCT
  TACTGCGCCGCGATTCGCCAGGCTGCACGGCATAAGCGGGTGGTCACCACGGATTGGGGATCCGAATCCC
  TGGCTCCAGATAGACTTAATGAAGAAGCACCGGATCCGGGCCGTGGCCACACAGGGCTCCTTTAATTCTT
  GGGACTGGGTCACACGTTACATGCTACTCTACGGCGACCGAGTGGACAGCTGGACACCGTTCTACCAGCG
  AGGGCACAACTCGACCTTCTTTGGTAACGTGAACGAGTCGGCGGTGGTGCGCCATGACCTGCACTTCCAC
  TTCACTGCGCGCTACATCCGCATCGTGCCCCTGGCCTGGAACCCACGCGGCAAGATCGGCCTGAGGCTCG
  GCCTCTATGGCTGCCCATACAAGGCCGACATACTCTATTTCGACGGCGACGATGCCATCTCCTACCGCTT
  CCCGCGAGGGGTCAGCCGAAGCCTGTGGGACGTGTTCGCCTTCAGCTTCAAGACCGAGGAGAAGGACGGT
  CTTCTGCTGCACGCCGAGGGCGCCCAGGGCGACTACGTGACGCTCGAGCTGGAGGGGGCACACCTGCTGC
  TGCACATGAGCCTGGGCAGCAGCCCTATCCAGCCAAGACCAGGTCACACCACCGTGAGCGCAGGCGGAGT
  CCTCAATGACCAGCACTGGCACTATGTGCGGGTGGACCGATTTGGCCGCGATGTAAATTTCACCCTGGAC
  GGCTATGTGCAGCGCTTTATTCTCAATGGAGACTTCGAGAGGCTGAACCTGGACACTGAGATGTTCATCG
  GAGGTCTGGTGGGCGCCGCGCGGAAGAACCTGGCCTATCGGCATAACTTCCGCGGCTGCATAGAAAACGT
  AATCTTCAACCGCGTCAACATCGCAGACCTGGCCGTGCGGCGCCATTCCCGGATCACCTTCGAGGGTAAG
  GTGGCTTTTCGTTGCCTGGACCCGGTACCGCACCCTATCAACTTCGGAGGCCCTCACAACTTCGTTCAAG
  TGCCCGGTTTCCCACGCCGTGGCCGCCTGGCAGTCTCATTTCGCTTCCGCACCTGGGACCTCACCGGGCT
  TCTCCTTTTCTCCCGTCTGGGGGACGGGCTGGGCCACGTGGAGCTGACGCTCAGCGAAGGGCAGGTCAAC
  GTGTCCATCGCGCAGAGCGGCCGAAAGAAGCTTCAGTTCGCTGCTGGGTACCGACTGAATGACGGCTTTT
  GGCACGAGGTGAATTTTGTGGCACAGGAAAACCATGCAGTTATCAGCATTGATGATGTGGAAGGGGCAGA
  GGTCAGGGTCTCATACCCGTTGCTGATCCGGACAGGGACCTCATATTTCTTTGGGGGTTGTCCCAAGCCA
  GCCAGTCGATGGGACTGCCACTCCAACCAGACGGCATTCCATGGCTGCATGGAGCTGCTCAAGGTGGATG
  GTCAACTGGTCAACCTGACTCTGGTGGAGGGCCGGCGGCTTGGATTCTATGCTGAGGTCCTCTTTGATAC
  ATGTGGCATCACTGATAGGTGCAGCCCTAACATGTGTGAGCATGATGGACGCTGCTACCAGTCTTGGGAT
  GACTTCATTTGCTACTGCGAACTGACGGGCTACAAGGGAGAGACCTGCCACACACCTTTGTATAAGGAAT
  CCTGTGAGGCTTATCGGCTCAGTGGGAAAACTTCTGGAAACTTCACCATTGATCCTGATGGCAGTGGCCC
  CCTGAAGCCATTTGTAGTGTACTGTGATATCCGAGAGAACCGAGCGTGGACAGTTGTGCGGCATGACAGG
  CTGTGGACAACTCGAGTGACAGGTTCCAGCATGGAGCGGCCATTCCTGGGGGCTATCCAGTACTGGAATG
  CATCCTGGGAGGAAGTCAGTGCCCTTGCCAATGCTTCCCAGCATTGTGAACAGTGGATCGAGTTCTCCTG
  CTACAATTCCCGGCTGCTCAACACTGCAGGAGGCTACCCCTACAGCTTTTGGATTGGCCGAAATGAGGAG
  CAGCACTTCTACTGGGGAGGCTCCCAGCCTGGGATCCAGCGCTGTGCCTGTGGTCTGGACCGGAGCTGTG
  TGGACCCTGCCTTGTACTGCAACTGTGACGCTGACCAGCCCCAGTGGAGAACTGACAAGGGACTGCTGAC
  CTTTGTGGACCATCTGCCTGTCACTCAGGTAGTGATAGGGGATACGAACCGCTCCACTTCTGAGGCCCAG
  TTCTTCCTGAGGCCTCTGCGCTGCTATGGCGATCGAAATTCCTGGAACACCATTTCCTTCCACACCGGGG
  CTGCACTACGCTTCCCCCCAATCCGTGCCAACCACAGCCTGGATGTCTCCTTCTACTTCAGGACCTCTGC
  TCCCTCGGGGGTCTTCCTAGAGAATATGGGGGGCCCTTACTGCCAGTGGCGCCGACCTTATGTGCGGGTG
  GAACTCAACACATCCCGGGATGTGGTCTTCGCCTTTGATGTGGGGAATGGGGATGAGAACCTCACAGTAC
  ACTCAGACGACTTTGAGTTCAATGATGACGAGTGGCACCTGGTCCGGGCTGAAATCAACGTGAAGCAGGC
  CCGGCTCCGAGTGGATCACCGGCCCTGGGTTCTGCGGCCTATGCCACTGCAGACCTACATCTGGATGGAG
  TATGACCAGCCCCTCTATGTGGGATCTGCAGAGCTTAAGAGACGCCCCTTTGTGGGTTGCTTGAGGGCCA
  TGCGTCTGAACGGAGTGACTCTGAACCTGGAGGGCCGTGCCAATGCCTCTGAGGGTACCTCACCCAACTG
  CACAGGCCACTGTGCCCACCCTCGGCTCCCCTGTTTCCATGGAGGCCGCTGCGTGGAGCGCTATAGCTAC
  TACACGTGTGACTGTGACCTCACGGCTTTTGATGGGCCATACTGCAACCACGATATTGGTGGTTTCTTTG
  AGCCGGGCACCTGGATGCGCTATAACCTACAGTCAGCGCTGCGCTCTGCAGCCAGGGAGTTCTCCCACAT
  GCTGAGCCGGCCAGTGCCAGGCTATGAGCCTGGCTACATCCCGGGCTATGATACTCCGGGCTATGTGCCT
  GGCTACCATGGCCCCGGGTACCGCCTGCCCGACTACCCCCGGCCTGGTCGGCCTGTGCCCGGTTACCGTG
  GGCCTGTCTACAACGTTACGGGAGAGGAGGTCTCCTTCAGCTTCAGCACCAGCTCCGCCCCTGCTGTCCT
  GCTCTACGTCAGTTCCTTTGTTCGTGACTACATGGCTGTGCTCATCAAGGATGATGGGACCCTTCAGCTG
  CGATATCAGCTGGGCACCAGTCCCTACGTGTACCAGCTAACCACTCGACCAGTGACCGATGGCCAGCCCC
  ATAGCATCAATATCACCCGTGTTTACCGGAACCTCTTCATCCAGGTGGACTACTTCCCACTGACAGAGCA
  GAAGTTCTCGCTGTTGGTGGACAGCCAGTTGGACTCACCCAAGGCCTTGTATTTAGGGCGTGTGATGGAG
  ACAGGAGTCATTGACCCGGAGATCCAGCGCTACAACACCCCAGGTTTCTCAGGCTGCCTGTCTGGTGTTC
  GATTCAACAACGTGGCTCCCCTCAAGACCCACTTCCGAACCCCTCGACCCATGACTGCTGAGCTAGCTGA
  GGCCCTTCGAGTTCAGGGAGAACTGTCCGAATCTAATTGCGGAGCTATGCCACGTCTTGTTTCAGAGGTG
  CCACCTGAGCTTGATCCCTGGTATCTGCCCCCAGACTTCCCCTACTACCATGATGAAGGATGGGTTGCCA
  TACTTTTAGGCTTTTTGGTGGCCTTTCTGCTGCTGGGGCTGGTGGGAATGTTGGTGCTCTTCTATCTGCA
  AAATCATCGCTATAAGGGCTCCTACCATACCAATGAGCCCAAGGCTGCCCACGAGTACCATCCTGGCAGC
  AAACCTCCCCTACCCACTTCAGGCCCTGCCCAGGTCCCCACCCCTACAGCAGCTCCCAACCAAGCTCCAG
  CCTCAGCCCCAGCCCCAGCCCCAACTCCAGCCCCAGCCCCTGGCCCCCGGGATCAGAACCTACCCCAGAT
  CCTGGAGGAGTCCAGGTCTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218019 representing NM_003632
  Red=Cloning site Green=Tags(s)
  
  MMHLRLFCILLAAVSGAEGWGYYGCDEELVGPLYARSLGASSYYSLLTAPRFARLHGISGWSPRIGDPNP
  WLQIDLMKKHRIRAVATQGSFNSWDWVTRYMLLYGDRVDSWTPFYQRGHNSTFFGNVNESAVVRHDLHFH
  FTARYIRIVPLAWNPRGKIGLRLGLYGCPYKADILYFDGDDAISYRFPRGVSRSLWDVFAFSFKTEEKDG
  LLLHAEGAQGDYVTLELEGAHLLLHMSLGSSPIQPRPGHTTVSAGGVLNDQHWHYVRVDRFGRDVNFTLD
  GYVQRFILNGDFERLNLDTEMFIGGLVGAARKNLAYRHNFRGCIENVIFNRVNIADLAVRRHSRITFEGK
  VAFRCLDPVPHPINFGGPHNFVQVPGFPRRGRLAVSFRFRTWDLTGLLLFSRLGDGLGHVELTLSEGQVN
  VSIAQSGRKKLQFAAGYRLNDGFWHEVNFVAQENHAVISIDDVEGAEVRVSYPLLIRTGTSYFFGGCPKP
  ASRWDCHSNQTAFHGCMELLKVDGQLVNLTLVEGRRLGFYAEVLFDTCGITDRCSPNMCEHDGRCYQSWD
  DFICYCELTGYKGETCHTPLYKESCEAYRLSGKTSGNFTIDPDGSGPLKPFVVYCDIRENRAWTVVRHDR
  LWTTRVTGSSMERPFLGAIQYWNASWEEVSALANASQHCEQWIEFSCYNSRLLNTAGGYPYSFWIGRNEE
  QHFYWGGSQPGIQRCACGLDRSCVDPALYCNCDADQPQWRTDKGLLTFVDHLPVTQVVIGDTNRSTSEAQ
  FFLRPLRCYGDRNSWNTISFHTGAALRFPPIRANHSLDVSFYFRTSAPSGVFLENMGGPYCQWRRPYVRV
  ELNTSRDVVFAFDVGNGDENLTVHSDDFEFNDDEWHLVRAEINVKQARLRVDHRPWVLRPMPLQTYIWME
  YDQPLYVGSAELKRRPFVGCLRAMRLNGVTLNLEGRANASEGTSPNCTGHCAHPRLPCFHGGRCVERYSY
  YTCDCDLTAFDGPYCNHDIGGFFEPGTWMRYNLQSALRSAAREFSHMLSRPVPGYEPGYIPGYDTPGYVP
  GYHGPGYRLPDYPRPGRPVPGYRGPVYNVTGEEVSFSFSTSSAPAVLLYVSSFVRDYMAVLIKDDGTLQL
  RYQLGTSPYVYQLTTRPVTDGQPHSINITRVYRNLFIQVDYFPLTEQKFSLLVDSQLDSPKALYLGRVME
  TGVIDPEIQRYNTPGFSGCLSGVRFNNVAPLKTHFRTPRPMTAELAEALRVQGELSESNCGAMPRLVSEV
  PPELDPWYLPPDFPYYHDEGWVAILLGFLVAFLLLGLVGMLVLFYLQNHRYKGSYHTNEPKAAHEYHPGS
  KPPLPTSGPAQVPTPTAAPNQAPASAPAPAPTPAPAPGPRDQNLPQILEESRSE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003632
• ORF Size: 4152 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003632.1, NM_003632.2, NP_003623.1
• RefSeq Size: 5293 bp
• RefSeq ORF: 4155 bp
• Locus ID: 8506
• Domains: F5_F8_type_C, LamG, EGF
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• MW: 156.27 kDa
• Gene Summary: The gene product was initially identified as a 190-kD protein associated with the contactin-PTPRZ1 complex. The 1,384-amino acid protein, also designated p190 or CASPR for 'contactin-associated protein,' includes an extracellular domain with several putative protein-protein interaction domains, a putative transmembrane domain, and a 74-amino acid cytoplasmic domain. Northern blot analysis showed that the gene is transcribed predominantly in brain as a transcript of 6.2 kb, with weak expression in several other tissues tested. The architecture of its extracellular domain is similar to that of neurexins, and this protein may be the signaling subunit of contactin, enabling recruitment and activation of intracellular signaling pathways in neurons. [provided by RefSeq, Jan 2009]