ASCL4 (NM_203436) Human Tagged ORF Clone
CAT#: RC218928
- TrueORF®
ASCL4 (Myc-DDK-tagged)-Human achaete-scute complex homolog 4 (Drosophila) (ASCL4)
"NM_203436" in other vectors (5)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ASCL4 |
Synonyms | ASH-4; bHLHa44; HASH4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC218928 representing NM_203436.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATGGAGACGCGTAAACCGGCGGAACGGCTGGCCTTGCCATACTCGCTGCGCACCGCGCCCCTGGGC GTTCCGGGGACCCTGCCCGGACTCCCGCGGAGGGACCCCCTCAGGGTCGCCCTGCGTCTGGACGCCGCG TGCTGGGAGTGGGCGCGCAGCGGCTGCGCACGGGGATGGCAGTACTTGCCCGTGCCGCTGGACAGCGCC TTCGAGCCCGCCTTCCTCCGCAAGCGCAACGAGCGCGAGCGGCAGCGGGTGCGCTGCGTGAACGAGGGC TATGCGCGCCTCCGAGACCACCTGCCCCGGGAGCTGGCAGACAAGCGCCTCAGCAAAGTGGAGACGCTC CGCGCTGCCATCGACTACATCAAGCACCTGCAGGAGCTGCTGGAGCGCCAGGCCTGGGGGCTCGAGGGC GCGGCCGGCGCCGTCCCCCAGCGCAGGGCGGAATGCAACAGCGACGGGGAGTCCAAGGCCTCTTCGGCG CCTTCGCCCAGCAGCGAGCCCGAGGAGGGGGGCAGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA >Peptide sequence encoded by RC218928
Blue=ORF Red=Cloning site Green=Tag(s) MMETRKPAERLALPYSLRTAPLGVPGTLPGLPRRDPLRVALRLDAACWEWARSGCARGWQYLPVPLDSA FEPAFLRKRNERERQRVRCVNEGYARLRDHLPRELADKRLSKVETLRAAIDYIKHLQELLERQAWGLEG AAGAVPQRRAECNSDGESKASSAPSPSSEPEEGGS SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_203436 |
ORF Size | 519 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_203436.2, NP_982260.2 |
RefSeq Size | 2260 bp |
RefSeq ORF | 519 bp |
Locus ID | 121549 |
UniProt ID | Q6XD76 |
Cytogenetics | 12q23.3 |
MW | 19.4 kDa |
Gene Summary | Basic helix-loop-helix transcription factors, such as ASCL4, are essential for the determination of cell fate and the development and differentiation of numerous tissues (Jonsson et al., 2004 [PubMed 15475265]).[supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC308176 | ASCL4 (untagged)-Human achaete-scute complex homolog 4 (Drosophila) (ASCL4) |
USD 420.00 |
|
SC327747 | ASCL4 (untagged)-Human achaete-scute complex homolog 4 (Drosophila) (ASCL4) |
USD 420.00 |
|
RG218928 | ASCL4 (GFP-tagged) - Human achaete-scute complex homolog 4 (Drosophila) (ASCL4) |
USD 460.00 |
|
RC218928L3 | Lenti-ORF clone of ASCL4 (Myc-DDK-tagged)-Human achaete-scute complex homolog 4 (Drosophila) (ASCL4) |
USD 768.00 |
|
RC218928L4 | Lenti-ORF clone of ASCL4 (mGFP-tagged)-Human achaete-scute complex homolog 4 (Drosophila) (ASCL4) |
USD 768.00 |
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