ASPP1 (PPP1R13B) (NM_015316) Human Tagged ORF Clone

CAT#: RC221172

PPP1R13B (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 13B (PPP1R13B)

Product Images

Western blot validation of overexpression lysate (Cat# LY414647) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221172 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PPP1R13B protein (Cat# TP321172). The protein was produced from HEK293T cells transfected with PPP1R13B cDNA clone (Cat# RC221172) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPP1R13B
• Synonyms: ASPP1; p53BP2-like; p85
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221172 representing NM_015316
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCCGATGATATTAACTGTTTTCTTGAGCAACAATGAACAGATTTTAACAGAAGTTCCTATAACAC
  CGGAAACAACCTGTCGAGATGTTGTAGAATTTTGCAAGGAACCTGGAGAAGGCAGCTGCCATTTAGCTGA
  AGTGTGGAGGGGAAATGAACGTCCCATACCCTTTGATCATATGATGTACGAACATCTTCAGAAATGGGGT
  CCACGGAGGGAAGAAGTGAAATTTTTCCTTCGACACGAGGACTCCCCAACTGAGAACAGTGAACAAGGTG
  GCCGTCAGACCCAAGAGCAACGAACTCAGAGAAATGTAATAAATGTACCTGGAGAAAAACGTACTGAAAA
  TGGGGTTGGGAATCCACGTGTTGAACTTACCCTCTCAGAGCTCCAAGATATGGCAGCTAGGCAACAGCAG
  CAGATTGAAAATCAGCAGCAGATGTTGGTTGCCAAGGAACAGCGTTTACATTTTCTAAAGCAACAGGAGC
  GCCGTCAGCAGCAGTCTATTTCTGAAAATGAAAAGCTTCAGAAATTGAAAGAACGAGTTGAAGCCCAGGA
  GAACAAGCTGAAGAAAATTCGTGCAATGAGAGGACAAGTCGACTACAGCAAAATCATGAACGGCAATCTG
  TCTGCTGAAATAGAAAGGTTCAGTGCCATGTTCCAGGAAAAGAAGCAGGAAGTACAGACTGCAATTTTAA
  GGGTTGATCAGCTTAGTCAGCAATTGGAAGATTTAAAGAAAGGAAAACTGAATGGGTTCCAGTCTTACAA
  TGGCAAATTGACGGGACCAGCGGCGGTGGAGTTAAAAAGACTGTACCAAGAACTACAGATTCGTAACCAA
  CTTAACCAGGAACAAAATTCAAAACTTCAGCAGCAGAAGGAACTCTTAAATAAGCGCAACATGGAGGTGG
  CCATGATGGACAAGCGAATCAGTGAACTGCGTGAACGTCTCTATGGGAAAAAAATTCAGCTGAACCGTGT
  GAATGGCACGTCATCACCACAGTCCCCTCTGAGCACATCGGGCAGGGTCGCTGCTGTGGGGCCTTATATC
  CAGGTTCCCAGTGCCGGAAGCTTTCCTGTGCTGGGGGACCCTATAAAGCCCCAGTCTCTCAGTATTGCCT
  CAAATGCTGCTCATGGAAGATCCAAATCCGCTAATGATGGAAACTGGCCAACATTAAAACAGAATTCTAG
  CTCTTCCGTGAAACCAGTGCAGGTGGCCGGTGCAGACTGGAAGGATCCGAGCGTGGAGGGGTCTGTCAAG
  CAGGGCACTGTCTCCAGCCAGCCTGTGCCCTTCTCAGCACTGGGACCCACGGAGAAGCCGGGCATCGAGA
  TTGGTAAAGTGCCACCTCCCATCCCGGGTGTAGGCAAGCAGCTGCCTCCAAGCTATGGGACATACCCAAG
  TCCTACACCTCTGGGTCCTGGGTCGACAAGCTCCCTGGAAAGGAGGAAGGAAGGCAGCTTGCCCAGGCCC
  AGTGCAGGCCTGCCAAGTCGACAGAGGCCCACCCTGCTGCCCGCCACAGGCAGCACCCCCCAGCCAGGCT
  CCTCACAACAGATTCAGCAGAGGATTTCCGTACCGCCAAGTCCCACGTACCCGCCAGCGGGACCACCTGC
  ATTTCCAGCTGGGGACAGCAAGCCTGAACTCCCACTGACAGTGGCCATTAGGCCTTTCCTGGCTGATAAA
  GGGTCAAGGCCACAGTCTCCCAGGAAAGGACCCCAGACAGTGAATTCAAGTTCCATATACTCCATGTACC
  TCCAGCAAGCCACACCACCTAAGAATTACCAGCCGGCAGCACACAGCGCCTTAAATAAGTCAGTTAAAGC
  AGTGTATGGTAAGCCCGTTTTACCTTCGGGTTCAACCTCTCCATCGCCGCTGCCGTTTCTTCACGGGTCA
  CTGTCCACGGGCACACCACAGCCTCAGCCACCTTCAGAAAGTACTGAGAAAGAGCCTGAGCAGGATGGCC
  CCGCCGCCCCCGCAGATGGCAGCACCGTGGAGAGCCTGCCACGGCCACTCAGCCCCACCAAGCTCACGCC
  CATCGTGCATTCGCCACTGCGCTACCAGAGTGATGCAGACCTGGAGGCCCTCCGCAGGAAGCTGGCCAAC
  GCGCCCCGGCCCCTGAAAAAGCGCAGCTCCATCACAGAGCCCGAGGGCCCCGGCGGGCCCAACATCCAGA
  AGCTGCTGTACCAGCGCTTCAACACCCTGGCCGGTGGCATGGAGGGCACCCCTTTCTACCAGCCCAGCCC
  CTCCCAGGACTTCATGGGCACCTTGGCCGATGTGGACAATGGAAACACCAATGCCAATGGAAACCTGGAA
  GAGCTCCCCCCTGCCCAGCCCACAGCCCCACTCCCCGCTGAGCCTGCCCCGTCATCAGATGCCAATGATA
  ATGAGTTACCTTCCCCCGAACCAGAGGAGCTCATCTGTCCCCAAACCACCCACCAAACTGCCGAGCCGGC
  AGAGGACAATAACAACAACGTGGCCACGGTCCCCACCACGGAGCAGATCCCGAGTCCTGTGGCTGAGGCC
  CCATCTCCAGGGGAAGAGCAGGTCCCTCCAGCACCTCTTCCCCCTGCCAGCCACCCTCCTGCCACCTCCA
  CGAACAAGCGGACCAACTTGAAGAAGCCCAACTCGGAGCGGACGGGGCACGGGCTGAGAGTCCGGTTTAA
  CCCCCTGGCACTGCTCCTAGACGCGTCTCTGGAAGGAGAGTTCGATCTGGTGCAGAGGATCATCTATGAG
  GTGGAAGATCCCAGCAAGCCCAACGACGAAGGGATCACCCCACTGCACAACGCCGTCTGCGCCGGCCACC
  ATCACATCGTGAAGTTCCTGCTGGATTTTGGTGTCAACGTGAATGCTGCTGATAGTGATGGATGGACGCC
  GCTGCACTGCGCTGCCTCTTGTAACAGCGTTCACCTCTGCAAACAGCTGGTGGAGAGTGGTGCCGCCATT
  TTTGCCTCAACCATAAGCGACATTGAAACTGCTGCAGACAAGTGTGAGGAGATGGAGGAAGGCTACATCC
  AGTGCTCCCAGTTTCTATATGGGGTGCAGGAAAAGCTGGGTGTGATGAACAAAGGTGTGGCGTATGCTCT
  GTGGGACTACGAGGCCCAGAACAGTGACGAGCTGTCCTTCCACGAAGGGGACGCCCTCACCATCCTGAGG
  CGCAAGGACGAAAGCGAGACTGAGTGGTGGTGGGCTCGCCTTGGAGACCGGGAGGGCTATGTGCCCAAAA
  ACCTGCTGGGGCTGTATCCACGGATCAAACCCCGACAGCGAACACTCGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221172 representing NM_015316
  Red=Cloning site Green=Tags(s)
  
  MMPMILTVFLSNNEQILTEVPITPETTCRDVVEFCKEPGEGSCHLAEVWRGNERPIPFDHMMYEHLQKWG
  PRREEVKFFLRHEDSPTENSEQGGRQTQEQRTQRNVINVPGEKRTENGVGNPRVELTLSELQDMAARQQQ
  QIENQQQMLVAKEQRLHFLKQQERRQQQSISENEKLQKLKERVEAQENKLKKIRAMRGQVDYSKIMNGNL
  SAEIERFSAMFQEKKQEVQTAILRVDQLSQQLEDLKKGKLNGFQSYNGKLTGPAAVELKRLYQELQIRNQ
  LNQEQNSKLQQQKELLNKRNMEVAMMDKRISELRERLYGKKIQLNRVNGTSSPQSPLSTSGRVAAVGPYI
  QVPSAGSFPVLGDPIKPQSLSIASNAAHGRSKSANDGNWPTLKQNSSSSVKPVQVAGADWKDPSVEGSVK
  QGTVSSQPVPFSALGPTEKPGIEIGKVPPPIPGVGKQLPPSYGTYPSPTPLGPGSTSSLERRKEGSLPRP
  SAGLPSRQRPTLLPATGSTPQPGSSQQIQQRISVPPSPTYPPAGPPAFPAGDSKPELPLTVAIRPFLADK
  GSRPQSPRKGPQTVNSSSIYSMYLQQATPPKNYQPAAHSALNKSVKAVYGKPVLPSGSTSPSPLPFLHGS
  LSTGTPQPQPPSESTEKEPEQDGPAAPADGSTVESLPRPLSPTKLTPIVHSPLRYQSDADLEALRRKLAN
  APRPLKKRSSITEPEGPGGPNIQKLLYQRFNTLAGGMEGTPFYQPSPSQDFMGTLADVDNGNTNANGNLE
  ELPPAQPTAPLPAEPAPSSDANDNELPSPEPEELICPQTTHQTAEPAEDNNNNVATVPTTEQIPSPVAEA
  PSPGEEQVPPAPLPPASHPPATSTNKRTNLKKPNSERTGHGLRVRFNPLALLLDASLEGEFDLVQRIIYE
  VEDPSKPNDEGITPLHNAVCAGHHHIVKFLLDFGVNVNAADSDGWTPLHCAASCNSVHLCKQLVESGAAI
  FASTISDIETAADKCEEMEEGYIQCSQFLYGVQEKLGVMNKGVAYALWDYEAQNSDELSFHEGDALTILR
  RKDESETEWWWARLGDREGYVPKNLLGLYPRIKPRQRTLA
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015316
• ORF Size: 3270 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015316.1, NM_015316.2, NP_056131.1, NP_056131.2
• RefSeq Size: 4834
• RefSeq ORF: 3273
• Locus ID: 23368
• Domains: SH3, ANK
• Protein Families: Druggable Genome
• MW: 119.4 kDa
• Gene Summary: This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. ASPP proteins are required for the induction of apoptosis by p53-family proteins. They promote DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor. [provided by RefSeq, Jul 2008]