AKAP5 (NM_004857) Human Tagged ORF Clone

CAT#: RC221314

AKAP5 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 5 (AKAP5)

Product Images

Western blot validation of overexpression lysate (Cat# LY401520) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221314 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AKAP5
• Synonyms: AKAP75; AKAP79; H21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221314 representing NM_004857
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACCACAATTTCAGAAATTCATGTAGAAAACAAGGATGAGAAGAGATCAGCAGAAGGTAGTCCTG
  GGGCTGAAAGGCAGAAGGAAAAGGCATCCATGCTTTGCTTCAAGAGAAGAAAGAAAGCAGCCAAAGCACT
  GAAGCCCAAAGCTGGCTCTGAAGCTGCTGATGTGGCAAGGAAGTGTCCACAAGAAGCAGGAGCTTCTGAT
  CAGCCAGAGCCCACACGGGGGGCCTGGGCCTCACTCAAACGTCTTGTAACACGCAGGAAAAGGTCAGAGT
  CTTCAAAGCAGCAAAAGCCATTGGAGGGTGAAATGCAACCTGCAATAAATGCTGAGGATGCTGATCTTTC
  TAAGAAAAAGGCAAAATCTAGACTTAAGATTCCCTGCATAAAATTCCCAAGAGGGCCAAAAAGGAGTAAT
  CATTCCAAAATTATAGAAGACTCAGACTGCAGCATCAAAGTCCAGGAAGAAGCTGAAATTTTGGATATAC
  AAACACAGACCCCATTGAATGATCAGGCAACAAAGGCTAAGTCAACCCAGGATCTAAGTGAAGGCATCTC
  ACGGAAAGATGGTGATGAGGTCTGTGAATCAAATGTGAGCAATAGCATAACTTCTGGAGAGAAAGTGATT
  TCAGTAGAACTTGGATTAGATAATGGGCATTCTGCTATTCAAACGGGAACTCTAATCCTTGAAGAAATTG
  AAACGATCAAGGAAAAACAAGATGTTCAACCCCAGCAAGCAAGCCCACTTGAAACTTCAGAAACAGACCA
  TCAGCAGCCAGTACTTTCTGATGTTCCTCCTTTACCTGCAATTCCAGATCAACAAATTGTGGAAGAAGCC
  AGTAACAGTACCCTAGAAAGTGCACCAAATGGAAAAGACTATGAAAGTACAGAGATTGTAGCTGAAGAAA
  CTAAGCCAAAAGATACTGAATTGAGCCAAGAATCAGATTTTAAAGAAAATGGGATCACTGAAGAGAAATC
  CAAATCAGAAGAAAGCAAAAGAATGGAGCCAATTGCTATTATTATTACAGACACTGAAATCAGTGAATTT
  GATGTTACAAAATCTAAAAATGTCCCTAAGCAATTCTTAATTTCAGCTGAAAATGAGCAAGTAGGGGTTT
  TTGCTAATGATAATGGTTTTGAGGATAGAACTTCAGAACAATATGAAACACTCTTAATTGAAACAGCCTC
  TTCTCTAGTCAAGAATGCTATTCAGTTGTCAATAGAACAGCTGGTTAATGAAATGGCCTCTGATGATAAT
  AAAATAAACAATCTTCTACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221314 representing NM_004857
  Red=Cloning site Green=Tags(s)
  
  METTISEIHVENKDEKRSAEGSPGAERQKEKASMLCFKRRKKAAKALKPKAGSEAADVARKCPQEAGASD
  QPEPTRGAWASLKRLVTRRKRSESSKQQKPLEGEMQPAINAEDADLSKKKAKSRLKIPCIKFPRGPKRSN
  HSKIIEDSDCSIKVQEEAEILDIQTQTPLNDQATKAKSTQDLSEGISRKDGDEVCESNVSNSITSGEKVI
  SVELGLDNGHSAIQTGTLILEEIETIKEKQDVQPQQASPLETSETDHQQPVLSDVPPLPAIPDQQIVEEA
  SNSTLESAPNGKDYESTEIVAEETKPKDTELSQESDFKENGITEEKSKSEESKRMEPIAIIITDTEISEF
  DVTKSKNVPKQFLISAENEQVGVFANDNGFEDRTSEQYETLLIETASSLVKNAIQLSIEQLVNEMASDDN
  KINNLLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004857
• ORF Size: 1281 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004857.1, NM_004857.2, NM_004857.3, NP_004848.2
• RefSeq Size: 2601 bp
• RefSeq ORF: 1284 bp
• Locus ID: 9495
• Domains: PkinA_anch
• Protein Families: Druggable Genome
• MW: 46.9 kDa
• Gene Summary: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT. [provided by RefSeq, Jul 2008]