AKAP5 (NM_004857) Human Tagged Lenti ORF Clone
CAT#: RC221314L2
- LentiORF®
Lenti ORF clone of Human A kinase (PRKA) anchor protein 5 (AKAP5), mGFP tagged
"NM_004857" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | AKAP5 |
Synonyms | AKAP75; AKAP79; H21 |
Vector | pLenti-C-mGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC221314).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_004857 |
ORF Size | 1281 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004857.2, NP_004848.2 |
RefSeq Size | 2601 bp |
RefSeq ORF | 1284 bp |
Locus ID | 9495 |
UniProt ID | P24588 |
Cytogenetics | 14q23.3 |
Domains | PkinA_anch |
Protein Families | Druggable Genome |
MW | 46.9 kDa |
Gene Summary | The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC117102 | AKAP5 (untagged)-Human A kinase (PRKA) anchor protein 5 (AKAP5) |
USD 760.00 |
|
RC221314 | AKAP5 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 5 (AKAP5) |
USD 420.00 |
|
RG221314 | AKAP5 (GFP-tagged) - Human A kinase (PRKA) anchor protein 5 (AKAP5) |
USD 460.00 |
|
RC221314L1 | Lenti ORF clone of Human A kinase (PRKA) anchor protein 5 (AKAP5), Myc-DDK-tagged |
USD 620.00 |
|
RC221314L3 | Lenti ORF clone of Human A kinase (PRKA) anchor protein 5 (AKAP5), Myc-DDK-tagged |
USD 620.00 |
|
RC221314L4 | Lenti ORF clone of Human A kinase (PRKA) anchor protein 5 (AKAP5), mGFP tagged |
USD 620.00 |
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