OVCA1 (DPH1) (NM_001383) Human Tagged ORF Clone

CAT#: RC221955

DPH1 (Myc-DDK-tagged)-Human DPH1 homolog (S. cerevisiae) (DPH1)

Product Images

Western blot validation of overexpression lysate (Cat# LY419989) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221955 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DPH1 protein (Cat# TP321955). The protein was produced from HEK293T cells transfected with DPH1 cDNA clone (Cat# RC221955) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DPH1
• Synonyms: DEDSSH; DPH2L; DPH2L1; OVCA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221955 representing NM_001383
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCAGGCAGGTGATGGCGGCGCTGGTCGTATCCGGGGCAGCGGAGCAGGGCGGCCGAGACGGCCCTG
  GCAGAGGTCGGGCCCCTCGGGGCCGCGTGGCCAATCAGATCCCCCCTGAGATCCTGAAGAACCCTCAGCT
  GCAGGCAGCAATCCGGGTCCTGCCTTCCAACTACAACTTTGAGATCCCCAAGACCATCTGGAGGATCCAA
  CAAGCCCAGGCCAAGAAGGTGGCCTTGCAAATGCCGGAAGGCCTCCTCCTCTTTGCCTGTACCATTGTGG
  ATATCTTGGAAAGGTTCACGGAGGCCGAAGTGATGGTGATGGGTGACGTGACCTACGGGGCTTGCTGTGT
  GGATGACTTCACAGCGAGGGCCCTGGGAGCTGACTTCTTGGTGCACTACGGCCACAGTTGCCTGATTCCC
  ATGGACACCTCGGCCCAAGACTTCCGGGTGCTGTACGTCTTTGTGGACATCCGGATAGACACTACACACC
  TCCTGGACTCTCTCCGCCTCACCTTTCCCCCAGCCACTGCCCTTGCCCTGGTCAGCACCATTCAGTTTGT
  GTCGACCTTGCAGGCAGCCGCCCAGGAGCTGAAAGCCGAGTATCGTGTGAGTGTCCCACAGTGCAAGCCC
  CTGTCCCCTGGAGAGATCCTGGGCTGCACATCCCCCCGACTGTCCAAAGAGGTGGAGGCCGTTGTGTATC
  TTGGAGATGGCCGCTTCCATCTGGAGTCTGTCATGATTGCCAACCCCAATGTCCCCGCTTACCGGTATGA
  CCCATATAGCAAAGTCCTATCCAGAGAACACTATGACCACCAGCGCATGCAGGCTGCTCGCCAAGAAGCC
  ATAGCCACTGCCCGCTCAGCTAAGTCCTGGGGCCTTATTCTGGGCACTTTGGGCCGCCAGGGCAGTCCTA
  AGATCCTGGAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAGGCTGCTGCTCTCTGA
  GATCTTCCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGTCTC
  TCCATTGACTGGGGCACAGCCTTCCCCAAGCCGCTGCTGACACCCTATGAGGCGGCCGTGGCTCTGAGGG
  ACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTCCTTGGGGCCCTGGACGGTGAA
  CCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCG
  CGTCCCCCTTCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221955 representing NM_001383
  Red=Cloning site Green=Tags(s)
  
  MRRQVMAALVVSGAAEQGGRDGPGRGRAPRGRVANQIPPEILKNPQLQAAIRVLPSNYNFEIPKTIWRIQ
  QAQAKKVALQMPEGLLLFACTIVDILERFTEAEVMVMGDVTYGACCVDDFTARALGADFLVHYGHSCLIP
  MDTSAQDFRVLYVFVDIRIDTTHLLDSLRLTFPPATALALVSTIQFVSTLQAAAQELKAEYRVSVPQCKP
  LSPGEILGCTSPRLSKEVEAVVYLGDGRFHLESVMIANPNVPAYRYDPYSKVLSREHYDHQRMQAARQEA
  IATARSAKSWGLILGTLGRQGSPKILEHLESRLRALGLSFVRLLLSEIFPSKLSLLPEVDVWVQVACPRL
  SIDWGTAFPKPLLTPYEAAVALRDISWQQPYPMDFYAGSSLGPWTVNHGQDRRPHAPGRPARGKVQEGSA
  RPPSAVACEDCSCRDEKVAPLAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001383
• ORF Size: 1329 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001383.2, NM_001383.3, NM_001383.4, NP_001374.3
• RefSeq Size: 2200 bp
• RefSeq ORF: 1332 bp
• Locus ID: 1801
• Cytogenetics: 17p13.3
• Domains: Diphthamide_syn
• MW: 48.6 kDa
• Gene Summary: 'The protein encoded by this gene is an enzyme involved in the biosynthesis of diphthamide, a modified histidine found only in elongation factor-2 (EEF2). Diphthamide residues in EEF2 are targeted for ADP-ribosylation by diphtheria toxin and Pseudomonas exotoxin A. Defects in this gene have been associated with both ovarian cancer and autosomal recessive intellectual disability with short stature, craniofacial, and ectodermal anomalies. [provided by RefSeq, Oct 2016]'