OVCA1 (DPH1) (NM_001383) Human Untagged Clone

CAT#: SC119252

DPH1 (untagged)-Human DPH1 homolog (S. cerevisiae) (DPH1)


  "NM_001383" in other vectors (7)

Reconstitution Protocol

USD 750.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol DPH1
Synonyms DEDSSH; DPH2L; DPH2L1; OVCA1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_001383, the custom clone sequence may differ by one or more nucleotides


ATGCGCAGGCAGGTGATGGCGGCGCTGGTCGTATCCGGGGCAGCGGAGCAGGGCGGCCGAGACGGCCCTG
GCAGAGGTCGGGCCCCTCGGGGCCGCGTGGCCAATCAGATCCCCCCTGAGATCCTGAAGAACCCTCAGCT
GCAGGCAGCAATCCGGGTCCTGCCTTCCAACTACAACTTTGAGATCCCCAAGACCATCTGGAGGATCCAA
CAAGCCCAGGCCAAGAAGGTGGCCTTGCAAATGCCGGAAGGCCTCCTCCTCTTTGCCTGTACCATTGTGG
ATATCTTGGAAAGGTTCACGGAGGCCGAAGTGATGGTGATGGGTGACGTGACCTACGGGGCTTGCTGTGT
GGATGACTTCACAGCGAGGGCCCTGGGAGCTGACTTCTTGGTGCACTACGGCCACAGTTGCCTGATTCCC
ATGGACACCTCGGCCCAAGACTTCCGGGTGCTGTACGTCTTTGTGGACATCCGGATAGACACTACACACC
TCCTGGACTCTCTCCGCCTCACCTTTCCCCCAGCCACTGCCCTTGCCCTGGTCAGCACCATTCAGTTTGT
GTCGACCTTGCAGGCAGCCGCCCAGGAGCTGAAAGCCGAGTATCGTGTGAGTGTCCCACAGTGCAAGCCC
CTGTCCCCTGGAGAGATCCTGGGCTGCACATCCCCCCGACTGTCCAAAGAGGTGGAGGCCGTTGTGTATC
TTGGAGATGGCCGCTTCCATCTGGAGTCTGTCATGATTGCCAACCCCAATGTCCCCGCTTACCGGTATGA
CCCATATAGCAAAGTCCTATCCAGAGAACACTATGACCACCAGCGCATGCAGGCTGCTCGCCAAGAAGCC
ATAGCCACTGCCCGCTCAGCTAAGTCCTGGGGCCTTATTCTGGGCACTTTGGGCCGCCAGGGCAGTCCTA
AGATCCTGGAGCACCTGGAATCTCGACTCCGAGCCTTGGGCCTTTCCTTTGTGAGGCTGCTGCTCTCTGA
GATCTTCCCCAGCAAGCTTAGCCTACTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGTCTC
TCCATTGACTGGGGCACAGCCTTCCCCAAGCCGCTGCTGACACCCTATGAGGCGGCCGTGGCTCTGAGGG
ACATTTCCTGGCAGCAGCCCTACCCGATGGACTTCTACGCTGGCAGCTCCTTGGGGCCCTGGACGGTGAA
CCACGGCCAGGACCGCCGTCCCCACGCCCCGGGCCGGCCCGCGCGGGGGAAGGTGCAGGAGGGGTCCGCG
CGTCCCCCTTCGGCCGTGGCTTGCGAGGACTGCAGCTGCAGGGACGAGAAGGTGGCGCCGCTGGCTCCTT
GA


>OriGene 5' read for NM_001383 unedited
GTGCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACTGATGGCGGCGCTG
GTCGTATCCGGGGCAGCGGAGCAGGGCGGCCGAGACGGCCCTGGCAGAGGTCGGGCCCCT
CGGGGCCGCGTGGCCAATCAGATCCCCCCTGAGATCCTGAAGAACCCTCAGCTGCAGGCA
GCAATCCGGGTCCTGCCTTCCAACTACAACTTTGAGATCCCCAAGACCATCTGGAGGATC
CAACAAGCCCAGGCCAAGAAGGTGGCCTTGCAAATGCCGGAAGGCCTCCTCCTCTTTGCC
TGTACCATTGTGGATATCTTGGAAAGGTTCACGGAGGCCGAAGTGATGGTGATGGGTGAC
GTGACCTACGGGGCTTGCTGTGTGGATGACTTCACAGCGAGGGCCCTGGGAGCTGACTTC
TTGGTGCACTACGGCCACAGTTGCCTGATTCCCATGGACACCTCGGCCCAAGACTTCCGG
GTGCTGTACGTCTTTGTGGACATCCGGATAGACACTACACACCTCCTGGACTCTCTCCGC
CTCACCTTTCCCCCAGCCACTGCCCTTGCCCTGGTCAGCCCATTTCAGTTTTGTGTCGAC
CTTGCANGCAGCCGCCCAGGAGCTGAAAGCCGAGTATCGTGTGAGTGTCCCACAGTGCAA
GCCCCTGTCCCCTGGAGAGATCCTGGGCTGCACATCCCCCCGACTGTCCAAAGAGGTGGG
AGGCCGTTGTGTATCTTGGAGATGGCCGCCTTCATCTGGAGTCTGTCATGATTGCCAACC
CCATGTCCCCGCTTACCGGTATGACCCATATAGCANAGTCCTATCCAGAGAACACTATGA
CCACCAGCGCATGCAGGCTGCTCGCCAAGAAGCCATAGCCACTGGCCGCTCAGCTAGTTC
TGGGGCCTTATTCTGGGCACTT
>OriGene 3' read for NM_001383 unedited
TATGTACCGCGGCACGCAATCTAGAATTTTTTTTTTTTTTTTTTTTCCAAATCACCACTT
TATTATGTGGCAGTCACCATGGTGCCAGCCTCTGCCTTCCCCATGTCACCCTCCTAATCC
AGGGCTCTCCAACCATATTCATGTTTGCGTTTTCACAAGTCAATGTCCATTAAGGCCTTG
TGCTCCCCTTTTCTTCAGTACAGGGCAGGGCCCTTATAATTTGGGTCTCAAGAATTGATA
GTTGTCTCTTAACTGGTTCTGGTGAGGCGCACTCACAGCAGTGAGTGGAGGGTCCTGGGA
GGGCATGGATGACGGAGGCTGCCCCTAAAGGAGCTGGATGTAGGACCAAAGACATTTCTT
GATCTTTCACTCTGCAAACTGGTCCAAGAACTTGAGGCAGGCCCGACGCTGGGGTGCACC
CTGCCGGAATGAAATGGCCCCCACAGTGGGCTCAGGGCGACTCCCCCTTCTAAACGACCG
GCCCCGTCCCCCCCCACCCTCCACCACCCCTACTGCCCCTCCCCCCCAACTTCGCATCAT
TTCGCCCCCGCCCCCCCGTACCCCGCCCCCCCCCTTCTCATTCTCCCCTGCCTCTCTCCC
CATAGACCCCTCTAACCCCCACCCTACCTCTCCCCCCATCATTCCCTGCACTCTGTATCC
CCCACCGCGCCGTCTTCTATACCACACCTCTCCCCACCACTCTCCCGCTCTCTTCTCTTT
AACACTNTACCCCCCCACACTACTCTCCTTTCCATCCCCNACCCTCCCCCGACCCGTTTC
CTCCTCCGCCCTCTTCACCCGGCCTTCTCACCTACCCCTCACTCACCTATTACTCACCCA
CGCCTCACTCGCACCCCCCCCATCTACACGCTATCTTCCCCTCCTCCTCACACTCACCCC
CATACAACTACATCGTCCCCTCAACAGATTACTACTACCTCACTCTCACCCCTCACTCCA
TTTTTCTCATCTCCTTCTTCT
Restriction Sites NotI-NotI     
ACCN NM_001383
Insert Size 2550 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001383.1, NP_001374.1
RefSeq Size 2200 bp
RefSeq ORF 1092 bp
Locus ID 1801
Cytogenetics 17p13.3
Domains Diphthamide_syn
Gene Summary 'The protein encoded by this gene is an enzyme involved in the biosynthesis of diphthamide, a modified histidine found only in elongation factor-2 (EEF2). Diphthamide residues in EEF2 are targeted for ADP-ribosylation by diphtheria toxin and Pseudomonas exotoxin A. Defects in this gene have been associated with both ovarian cancer and autosomal recessive intellectual disability with short stature, craniofacial, and ectodermal anomalies. [provided by RefSeq, Oct 2016]'
Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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