Thrombospondin 2 (THBS2) (NM_003247) Human Tagged ORF Clone

CAT#: RC223225

THBS2 (Myc-DDK-tagged)-Human thrombospondin 2 (THBS2)

Product Images

Western blot validation of overexpression lysate (Cat# LY418810) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223225 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: THBS2
• Synonyms: TSP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223225 representing NM_003247
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCTGGAGGCTGGTCCTGCTGGCTCTGTGGGTGTGGCCCAGCACGCAAGCTGGTCACCAGGACAAAG
  ACACGACCTTCGACCTTTTCAGTATCAGCAACATCAACCGCAAGACCATTGGCGCCAAGCAGTTCCGCGG
  GCCCGACCCCGGCGTGCCGGCTTACCGCTTCGTGCGCTTTGACTACATCCCACCGGTGAACGCAGATGAC
  CTCAGCAAGATCACCAAGATCATGCGGCAGAAGGAGGGCTTCTTCCTCACGGCCCAGCTCAAGCAGGACG
  GCAAGTCCAGGGGCACGCTGTTGGCTCTGGAGGGCCCCGGTCTCTCCCAGAGGCAGTTCGAGATCGTCTC
  CAACGGCCCCGCGGACACGCTGGATCTCACCTACTGGATTGACGGCACCCGGCATGTGGTCTCCCTGGAG
  GACGTCGGCCTGGCTGACTCGCAGTGGAAGAACGTCACCGTGCAGGTGGCTGGCGAGACCTACAGCTTGC
  ACGTGGGCTGCGACCTCATAGACAGCTTCGCTCTGGACGAGCCCTTCTACGAGCACCTGCAGGCGGAAAA
  GAGCCGGATGTACGTGGCCAAAGGCTCTGCCAGAGAGAGTCACTTCAGGGGTTTGCTTCAGAACGTCCAC
  CTAGTGTTTGAAAACTCTGTGGAAGATATTCTAAGCAAGAAGGGTTGCCAGCAAGGCCAGGGAGCTGAGA
  TCAACGCCATCAGTGAGAACACAGAGACGCTGCGCCTGGGTCCGCATGTCACCACCGAGTACGTGGGCCC
  CAGCTCGGAGAGGAGGCCCGAGGTGTGCGAACGCTCGTGCGAGGAGCTGGGAAACATGGTCCAGGAGCTC
  TCGGGGCTCCACGTCCTCGTGAACCAGCTCAGCGAGAACCTCAAGAGAGTGTCGAATGATAACCAGTTTC
  TCTGGGAGCTCATTGGTGGCCCTCCTAAGACAAGGAACATGTCAGCTTGCTGGCAGGATGGCCGGTTCTT
  TGCGGAAAATGAAACGTGGGTGGTGGACAGCTGCACCACGTGTACCTGCAAGAAATTTAAAACCATTTGC
  CACCAAATCACCTGCCCGCCTGCAACCTGCGCCAGTCCATCCTTTGTGGAAGGCGAATGCTGCCCTTCCT
  GCCTCCACTCGGTGGACGGTGAGGAGGGCTGGTCTCCGTGGGCAGAGTGGACCCAGTGCTCCGTGACGTG
  TGGCTCTGGGACCCAGCAGAGAGGCCGGTCCTGTGACGTCACCAGCAACACCTGCTTGGGGCCCTCCATC
  CAGACACGGGCTTGCAGTCTGAGCAAGTGTGACACCCGCATCCGGCAGGACGGCGGCTGGAGCCACTGGT
  CACCTTGGTCTTCATGCTCTGTGACCTGTGGAGTTGGCAATATCACACGCATCCGTCTCTGCAACTCCCC
  AGTGCCCCAGATGGGGGGCAAGAATTGCAAAGGGAGTGGCCGGGAGACCAAAGCCTGCCAGGGCGCCCCA
  TGCCCAATCGATGGCCGCTGGAGCCCCTGGTCCCCGTGGTCGGCCTGCACTGTCACCTGTGCCGGTGGGA
  TCCGGGAGCGCACCCGGGTCTGCAACAGCCCTGAGCCTCAGTACGGAGGGAAGGCCTGCGTGGGGGATGT
  GCAGGAGCGTCAGATGTGCAACAAGAGGAGCTGCCCCGTGGATGGCTGTTTATCCAACCCCTGCTTCCCG
  GGAGCCCAGTGCAGCAGCTTCCCCGATGGGTCCTGGTCATGCGGCTCCTGCCCTGTGGGCTTCTTGGGCA
  ATGGCACCCACTGTGAGGACCTGGACGAGTGTGCCCTGGTCCCCGACATCTGCTTCTCCACCAGCAAGGT
  GCCTCGCTGTGTCAACACTCAGCCTGGCTTCCACTGCCTGCCCTGCCCGCCCCGATACAGAGGGAACCAG
  CCCGTCGGGGTCGGCCTGGAAGCAGCCAAGACGGAAAAGCAAGTGTGTGAGCCCGAAAACCCATGCAAGG
  ACAAGACACACAACTGCCACAAGCACGCGGAGTGCATCTACCTGGGCCACTTCAGCGACCCCATGTACAA
  GTGCGAGTGCCAGACAGGCTACGCGGGCGACGGGCTCATCTGCGGGGAGGACTCGGACCTGGACGGCTGG
  CCCAACCTCAATCTGGTCTGCGCCACCAACGCCACCTACCACTGCATCAAGGATAACTGCCCCCATCTGC
  CAAATTCTGGGCAGGAAGACTTTGACAAGGACGGGATTGGCGATGCCTGTGATGATGACGATGACAATGA
  CGGTGTGACCGATGAGAAGGACAACTGCCAGCTCCTCTTCAATCCCCGCCAGGCTGACTATGACAAGGAT
  GAGGTTGGGGACCGCTGTGACAACTGCCCTTACGTGCACAACCCTGCCCAGATCGACACAGACAACAATG
  GAGAGGGTGACGCCTGCTCCGTGGACATTGATGGGGACGATGTCTTCAATGAACGAGACAATTGTCCCTA
  CGTCTACAACACTGACCAGAGGGACACGGATGGTGACGGTGTGGGGGATCACTGTGACAACTGCCCCCTG
  GTGCACAACCCTGACCAGACCGACGTGGACAATGACCTTGTTGGGGACCAGTGTGACAACAACGAGGACA
  TAGATGACGACGGCCACCAGAACAACCAGGACAACTGCCCCTACATCTCCAACGCCAACCAGGCTGACCA
  TGACAGAGACGGCCAGGGCGACGCCTGTGACCCTGATGATGACAACGATGGCGTCCCCGATGACAGGGAC
  AACTGCCGGCTTGTGTTCAACCCAGACCAGGAGGACTTGGACGGTGATGGACGGGGTGATATTTGTAAAG
  ATGATTTTGACAATGACAACATCCCAGATATTGATGATGTGTGTCCTGAAAACAATGCCATCAGTGAGAC
  AGACTTCAGGAACTTCCAGATGGTCCCCTTGGATCCCAAAGGGACCACCCAAATTGATCCCAACTGGGTC
  ATTCGCCATCAAGGCAAGGAGCTGGTTCAGACAGCCAACTCGGACCCCGGCATCGCTGTAGGTTTTGACG
  AGTTTGGGTCTGTGGACTTCAGTGGCACATTCTACGTAAACACTGACCGGGACGACGACTATGCCGGCTT
  CGTCTTTGGTTACCAGTCAAGCAGCCGCTTCTATGTGGTGATGTGGAAGCAGGTGACGCAGACCTACTGG
  GAGGACCAGCCCACGCGGGCCTATGGCTACTCCGGCGTGTCCCTCAAGGTGGTGAACTCCACCACGGGGA
  CGGGCGAGCACCTGAGGAACGCGCTGTGGCACACGGGGAACACGCCGGGGCAGGTGCGAACCTTATGGCA
  CGACCCCAGGAACATTGGCTGGAAGGACTACACGGCCTATAGGTGGCACCTGACTCACAGGCCCAAGACT
  GGCTACATCAGAGTCTTAGTGCATGAAGGAAAACAGGTCATGGCAGACTCAGGACCTATCTATGACCAAA
  CCTACGCTGGCGGGCGGCTGGGTCTATTTGTCTTCTCTCAAGAAATGGTCTATTTCTCAGACCTCAAGTA
  CGAATGCAGAGATATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223225 representing NM_003247
  Red=Cloning site Green=Tags(s)
  
  MVWRLVLLALWVWPSTQAGHQDKDTTFDLFSISNINRKTIGAKQFRGPDPGVPAYRFVRFDYIPPVNADD
  LSKITKIMRQKEGFFLTAQLKQDGKSRGTLLALEGPGLSQRQFEIVSNGPADTLDLTYWIDGTRHVVSLE
  DVGLADSQWKNVTVQVAGETYSLHVGCDLIDSFALDEPFYEHLQAEKSRMYVAKGSARESHFRGLLQNVH
  LVFENSVEDILSKKGCQQGQGAEINAISENTETLRLGPHVTTEYVGPSSERRPEVCERSCEELGNMVQEL
  SGLHVLVNQLSENLKRVSNDNQFLWELIGGPPKTRNMSACWQDGRFFAENETWVVDSCTTCTCKKFKTIC
  HQITCPPATCASPSFVEGECCPSCLHSVDGEEGWSPWAEWTQCSVTCGSGTQQRGRSCDVTSNTCLGPSI
  QTRACSLSKCDTRIRQDGGWSHWSPWSSCSVTCGVGNITRIRLCNSPVPQMGGKNCKGSGRETKACQGAP
  CPIDGRWSPWSPWSACTVTCAGGIRERTRVCNSPEPQYGGKACVGDVQERQMCNKRSCPVDGCLSNPCFP
  GAQCSSFPDGSWSCGSCPVGFLGNGTHCEDLDECALVPDICFSTSKVPRCVNTQPGFHCLPCPPRYRGNQ
  PVGVGLEAAKTEKQVCEPENPCKDKTHNCHKHAECIYLGHFSDPMYKCECQTGYAGDGLICGEDSDLDGW
  PNLNLVCATNATYHCIKDNCPHLPNSGQEDFDKDGIGDACDDDDDNDGVTDEKDNCQLLFNPRQADYDKD
  EVGDRCDNCPYVHNPAQIDTDNNGEGDACSVDIDGDDVFNERDNCPYVYNTDQRDTDGDGVGDHCDNCPL
  VHNPDQTDVDNDLVGDQCDNNEDIDDDGHQNNQDNCPYISNANQADHDRDGQGDACDPDDDNDGVPDDRD
  NCRLVFNPDQEDLDGDGRGDICKDDFDNDNIPDIDDVCPENNAISETDFRNFQMVPLDPKGTTQIDPNWV
  IRHQGKELVQTANSDPGIAVGFDEFGSVDFSGTFYVNTDRDDDYAGFVFGYQSSSRFYVVMWKQVTQTYW
  EDQPTRAYGYSGVSLKVVNSTTGTGEHLRNALWHTGNTPGQVRTLWHDPRNIGWKDYTAYRWHLTHRPKT
  GYIRVLVHEGKQVMADSGPIYDQTYAGGRLGLFVFSQEMVYFSDLKYECRDI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003247
• ORF Size: 3516 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003247.1, NM_003247.2, NM_003247.3, NP_003238.2
• RefSeq Size: 5826 bp
• RefSeq ORF: 3519 bp
• Locus ID: 7058
• Domains: tsp_1, VWC, EGF_CA, TSPN, tsp_3, EGF, EGF
• Protein Families: Druggable Genome
• Protein Pathways: ECM-receptor interaction, Focal adhesion, TGF-beta signaling pathway
• MW: 129.99 kDa
• Gene Summary: The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. [provided by RefSeq, Jul 2008]