GPR105 (P2RY14) (NM_001081455) Human Tagged ORF Clone
CAT#: RC224391
P2RY14 (Myc-DDK-tagged)-Human purinergic receptor P2Y, G-protein coupled, 14 (P2RY14), transcript variant 1
"NM_001081455" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | P2RY14 |
Synonyms | BPR105; GPR105; P2Y14 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224391 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCAATTCAACCTCCACACAGCCTCCAGATGAATCCTGCTCTCAGAACCTCCTGATCACTCAGCAGA TCATTCCTGTGCTGTACTGTATGGTCTTCATTGCAGGAATCCTACTCAATGGAGTGTCAGGATGGATATT CTTTTACGTGCCCAGCTCTGAGAGTTTCATCATCTATCTCAAGAACATTGTTATTGCTGACTTTGTGATG AGCCTGACTTTTCCTTTCAAGATCCTTGGTGACTCAGGCCTTGGTCCCTGGCAGCTGAACGTGTTTGTGT GCAGGGTCTCTGCCGTGCTCTTCTACGTCAACATGTACGTCAGCATTGTGTTCTTTGGGCTCATCAGCTT TGACAGATATTATAAAATTGTAAAGCCTCTTTGGACTTCTTTCATCCAGTCAGTGAGTTACAGCAAACTT CTGTCAGTGATAGTATGGATGCTCATGCTCCTCCTTGCTGTTCCAAATATTATTCTCACCAACCAGAGTG TTAGGGAGGTTACACAAATAAAATGTATAGAACTGAAAAGTGAACTGGGACGGAAGTGGCACAAAGCATC AAACTACATCTTCGTGGCCATCTTCTGGATTGTGTTTCTTTTGTTAATCGTTTTCTATACTGCTATCACA AAGAAAATCTTTAAGTCCCACCTTAAGTCAAGTCGGAATTCCACTTCGGTCAAAAAGAAATCTAGCCGCA ACATATTCAGCATCGTGTTTGTGTTTTTTGTCTGTTTTGTACCTTACCATATTGCCAGAATCCCCTACAC AAAGAGTCAGACCGAAGCTCATTACAGCTGCCAGTCAAAAGAAATCTTGCGGTATATGAAAGAATTCACT CTGCTACTATCTGCTGCAAATGTATGCTTGGACCCTATTATTTATTTCTTTCTATGCCAGCCGTTTAGGG AAATCTTATGTAAGAAATTGCACATTCCATTAAAAGCTCAGAATGACCTAGACATTTCCAGAATCAAAAG AGGAAATACAACACTTGAAAGCACAGATACTTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224391 protein sequence
Red=Cloning site Green=Tags(s) MINSTSTQPPDESCSQNLLITQQIIPVLYCMVFIAGILLNGVSGWIFFYVPSSESFIIYLKNIVIADFVM SLTFPFKILGDSGLGPWQLNVFVCRVSAVLFYVNMYVSIVFFGLISFDRYYKIVKPLWTSFIQSVSYSKL LSVIVWMLMLLLAVPNIILTNQSVREVTQIKCIELKSELGRKWHKASNYIFVAIFWIVFLLLIVFYTAIT KKIFKSHLKSSRNSTSVKKKSSRNIFSIVFVFFVCFVPYHIARIPYTKSQTEAHYSCQSKEILRYMKEFT LLLSAANVCLDPIIYFFLCQPFREILCKKLHIPLKAQNDLDISRIKRGNTTLESTDTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001081455 |
ORF Size | 1014 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001081455.1, NP_001074924.1 |
RefSeq Size | 2694 bp |
RefSeq ORF | 1017 bp |
Locus ID | 9934 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Protein Pathways | Neuroactive ligand-receptor interaction |
MW | 39 kDa |
Gene Summary | The product of this gene belongs to the family of G-protein coupled receptors, which contains several receptor subtypes with different pharmacological selectivity for various adenosine and uridine nucleotides. This receptor is a P2Y purinergic receptor for UDP-glucose and other UDP-sugars coupled to G-proteins. It has been implicated in extending the known immune system functions of P2Y receptors by participating in the regulation of the stem cell compartment, and it may also play a role in neuroimmune function. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC315940 | P2RY14 (untagged)-Human purinergic receptor P2Y, G-protein coupled, 14 (P2RY14), transcript variant 1 |
USD 760.00 |
|
RG224391 | P2RY14 (GFP-tagged) - Human purinergic receptor P2Y, G-protein coupled, 14 (P2RY14), transcript variant 1 |
USD 460.00 |
|
RC224391L1 | Lenti ORF clone of Human purinergic receptor P2Y, G-protein coupled, 14 (P2RY14), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC224391L2 | Lenti ORF clone of Human purinergic receptor P2Y, G-protein coupled, 14 (P2RY14), transcript variant 1, mGFP tagged |
USD 768.00 |
|
RC224391L3 | Lenti ORF clone of Human purinergic receptor P2Y, G-protein coupled, 14 (P2RY14), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC224391L4 | Lenti ORF clone of Human purinergic receptor P2Y, G-protein coupled, 14 (P2RY14), transcript variant 1, mGFP tagged |
USD 620.00 |
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