VAX1 (NM_001112704) Human Tagged ORF Clone

CAT#: RC225487

VAX1 (Myc-DDK-tagged)-Human ventral anterior homeobox 1 (VAX1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY426368) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225487 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VAX1
• Synonyms: MCOPS11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225487 representing NM_001112704
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGGGAAACCAGACAAAATGGACGTTCGATGCCACTCGGACGCCGAGGCTGCCCGGGTCTCGAAGA
  ACGCGCACAAGGAGAGTCGGGAGAGCAAGGGCGCGGAGGGGAACCTCCCAGCCGCCTTCCTCAAGGAGCC
  GCAGGGCGCCTTCTCAGCGTCGGGCGCTGCTGAGGATTGTAACAAAAGTAAATCCAATTCCGCAGCGGAC
  CCGGATTACTGCCGCCGGATCCTGGTCCGAGATGCCAAGGGGTCCATCCGAGAGATCATCCTGCCCAAGG
  GCCTGGACTTGGACCGGCCTAAGAGGACGCGCACGTCCTTCACCGCGGAGCAGCTCTATCGGCTGGAGAT
  GGAGTTCCAGCGCTGCCAGTACGTGGTGGGCCGCGAGAGGACCGAGCTCGCCCGGCAGCTTAACCTCTCC
  GAGACCCAGGTGAAGGTCTGGTTCCAGAACCGGCGCACCAAGCAGAAGAAGGACCAGGGCAAGGACTCGG
  AGCTACGCTCGGTGGTGTCGGAGACCGCGGCCACGTGCAGCGTGCTACGGCTGCTGGAGCAGGGCCGCCT
  GTTGTCGCCGCCCGGCCTGCCTGCGCTGCTGCCGCCTTGCGCCACGGGCGCTCTCGGCTCAGCGCTGCGC
  GGGCCCAGCTTGCCGGCCCTGGGCGCGGGCGCCGCTGCAGGCTCGGCCGCCGCAGCCGCCGCCGCCGCCC
  CGGGCCCAGCGGGCGCTGCATCCCCGCACCCGCCGGCTGTGGGCGGTGCTCCAGGTCCCGGGCCCGCCGG
  GCCGGGGGGATTGCACGCAGGCGCCCCGGCCGCGGGCCACAGCCTCTTCAGCCTGCCGGTGCCCTCGCTG
  CTCGGCTCCGTCGCCAGCCGCCTGTCCTCCGCCCCGTTAACAATGGCTGGTTCGCTAGCTGGGAATTTGC
  AAGAACTCTCCGCCCGATATCTGAGCTCCTCGGCCTTCGAGCCTTACTCCCGGACCAACAATAAAGAAGG
  GGCCGAGAAAAAAGCGCTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225487 representing NM_001112704
  Red=Cloning site Green=Tags(s)
  
  MFGKPDKMDVRCHSDAEAARVSKNAHKESRESKGAEGNLPAAFLKEPQGAFSASGAAEDCNKSKSNSAAD
  PDYCRRILVRDAKGSIREIILPKGLDLDRPKRTRTSFTAEQLYRLEMEFQRCQYVVGRERTELARQLNLS
  ETQVKVWFQNRRTKQKKDQGKDSELRSVVSETAATCSVLRLLEQGRLLSPPGLPALLPPCATGALGSALR
  GPSLPALGAGAAAGSAAAAAAAAPGPAGAASPHPPAVGGAPGPGPAGPGGLHAGAPAAGHSLFSLPVPSL
  LGSVASRLSSAPLTMAGSLAGNLQELSARYLSSSAFEPYSRTNNKEGAEKKALD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001112704
• ORF Size: 1002 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001112704.1, NP_001106175.1
• RefSeq ORF: 1005
• Locus ID: 11023
• Protein Families: Transcription Factors
• MW: 34.5 kDa
• Gene Summary: This gene encodes a homeo-domain containing protein from a class of homeobox transcription factors which are conserved in vertebrates. Genes of this family are involved in the regulation of body development and morphogenesis. The most conserved genes, called HOX genes are found in special gene clusters. This gene belongs to the VAX subfamily and lies in the vicinity of the EMX homeobox gene family. Another member of VAX family is located on chromosome 2. The encoded protein may play an important role in the development of anterior ventral forebrain and visual system. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]