TMEM16A (ANO1) (NM_018043) Human Tagged Lenti ORF Clone

CAT#: RC229400L1

Lenti-ORF clone of ANO1 (Myc-DDK-tagged)-Human anoctamin 1, calcium activated chloride channel (ANO1), transcript variant 1

Product Images

Double digestion of RC229400L1 using SgfI and MluI

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  Double digestion of RC229400L1 using SgfI and MluI

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM16A
• Synonyms: DOG1; ORAOV2; TAOS2; TMEM16A
• Vector: pLenti-C-Myc-DDK
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC229400).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_018043
• ORF Size: 2958 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018043.5
• RefSeq ORF: 2961 bp
• Locus ID: 55107
• UniProt ID: Q5XXA6
• Cytogenetics: 11q13.3
• Domains: DUF590
• Protein Families: Transmembrane
• MW: 113.9 kDa
• Gene Summary: Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.[UniProtKB/Swiss-Prot Function]