TMEM16A (ANO1) (NM_018043) Human Untagged Clone

CAT#: SC328035

ANO1 (untagged)-Human anoctamin 1 calcium activated chloride channel (ANO1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ANO1
• Synonyms: DOG1; ORAOV2; TAOS2; TMEM16A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018043, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGGGTCAACGAGAAGTACTCGACGCTCCCGGCCGAGGACCGCAGCGTCCACATCATCAACATCTGCG
  CCATCGAGGACATCGGCTACCTGCCGTCCGAGGGCACGCTGCTGAACTCCTTATCTGTGGACCCTGATGC
  CGAGTGCAAGTATGGCCTGTACTTCAGGGACGGCCGGCGCAAGGTGGACTACATCCTGGTGTACCATCAC
  AAGAGGCCCTCGGGCAACCGGACCCTGGTCAGGAGGGTGCAGCACAGCGACACCCCCTCTGGGGCTCGCA
  GCGTCAAGCAGGACCACCCCCTGCCGGGCAAGGGGGCGTCGCTGGATGCAGGCTCGGGGGAGCCCCCGAT
  GGACTACCACGAGGATGACAAGCGCTTCCGCAGGGAGGAGTACGAGGGCAACCTCCTGGAGGCGGGCCTG
  GAGCTGGAGCGGGACGAGGACACTAAAATCCACGGAGTCGGGTTTGTGAAAATCCATGCCCCCTGGAACG
  TGCTGTGCAGAGAGGCCGAGTTTCTGAAACTGAAGATGCCGACGAAGAAGATGTACCACATTAATGAGAC
  CCGTGGCCTCCTGAAAAAAATCAACTCTGTGCTCCAGAAAATCACAGATCCCATCCAGCCCAAAGTGGCT
  GAGCACAGGCCCCAGACCATGAAGAGACTCTCCTATCCCTTCTCCCGGGAGAAGCAGCATCTATTTGACT
  TGTCTGATAAGGATTCCTTTTTCGACAGCAAAACCCGGAGCACGATTGTCTATGAGATCTTGAAGAGAAC
  GACGTGTACAAAGGCCAAGTACAGCATGGGCATCACGAGCCTGCTGGCCAATGGTGTGTACGCGGCTGCA
  TACCCACTGCACGATGGAGACTACAACGGTGAAAACGTCGAGTTCAACGACAGAAAACTCCTGTACGAAG
  AGTGGGCACGCTATGGAGTTTTCTATAAGTACCAGCCCATCGACCTGGTCAGGAAGTATTTTGGGGAGAA
  GATCGGCCTGTACTTCGCCTGGCTGGGCGTGTACACCCAGATGCTCATCCCTGCCTCCATCGTGGGAATC
  ATTGTCTTCCTGTACGGATGCGCCACCATGGATGAAAACATCCCCAGCATGGAGATGTGTGACCAGAGAC
  ACAATATCACCATGTGCCCGCTTTGCGACAAGACCTGCAGCTACTGGAAGATGAGCTCAGCCTGCGCCAC
  GGCCCGCGCCAGCCACCTCTTCGACAACCCCGCCACGGTCTTCTTCTCTGTCTTCATGGCCCTCTGGGCT
  GCCACCTTCATGGAGCACTGGAAGCGGAAACAGATGCGACTCAACTACCGCTGGGACCTCACGGGCTTTG
  AAGAGGAAGAGGAGGCTGTCAAGGATCATCCTAGAGCTGAATACGAAGCCAGAGTCTTGGAGAAGTCTCT
  GAAGAAAGAGTCCAGAAACAAAGAGAAGCGCCGGCATATTCCAGAGGAGTCAACAAACAAATGGAAGCAG
  AGGGTTAAGACAGCCATGGCGGGGGTGAAATTGACTGACAAAGTGAAGCTGACATGGAGAGATCGGTTCC
  CAGCCTACCTCACTAACTTGGTCTCCATCATCTTCATGATTGCAGTGACGTTTGCCATCGTCCTCGGCGT
  CATCATCTACAGAATCTCCATGGCCGCCGCCTTGGCCATGAACTCCTCCCCCTCCGTGCGGTCCAACATC
  CGGGTCACAGTCACAGCCACCGCAGTCATCATCAACCTAGTGGTCATCATCCTCCTGGACGAGGTGTATG
  GCTGCATAGCCCGATGGCTCACCAAGATCGAGGTCCCAAAGACGGAGAAAAGCTTTGAGGAGAGGCTGAT
  CTTCAAGGCTTTCCTGCTGAAGTTTGTGAATTCCTACACCCCCATCTTTTACGTGGCGTTCTTCAAAGGC
  CGGTTTGTTGGACGCCCGGGCGACTACGTGTACATTTTCCGTTCCTTCCGAATGGAAGAGTGTGCGCCAG
  GGGGCTGCCTGATGGAGCTATGCATCCAGCTCAGCATCATCATGCTGGGGAAACAGCTGATCCAGAACAA
  CCTGTTCGAGATCGGCATCCCGAAGATGAAGAAGCTCATCCGCTACCTGAAGCTGAAGCAGCAGAGCCCC
  CCTGACCACGAGGAGTGTGTGAAGAGGAAACAGCGGTACGAGGTGGATTACAACCTGGAGCCCTTCGCGG
  GCCTCACCCCAGAGTACATGGAAATGATCATCCAGTTTGGCTTCGTCACCCTGTTTGTCGCCTCCTTCCC
  CCTGGCCCCACTGTTTGCGCTGCTGAACAACATCATCGAGATCCGCCTGGACGCCAAAAAGTTTGTCACT
  GAGCTCCGAAGGCCGGTAGCTGTCAGAGCCAAAGACATCGGAATCTGGTACAATATCCTCAGAGGCATTG
  GGAAGCTTGCTGTCATCATCAATGCCTTCGTGATCTCCTTCACGTCTGACTTCATCCCGCGCCTGGTGTA
  CCTCTACATGTACAGTAAGAACGGGACCATGCACGGCTTCGTCAACCACACCCTCTCCTCCTTCAACGTC
  AGTGACTTCCAGAACGGCACGGCCCCCAATGACCCCCTGGACCTGGGCTACGAGGTGCAGATCTGCAGGT
  ATAAAGACTACCGAGAGCCGCCGTGGTCGGAAAACAAGTACGACATCTCCAAGGACTTCTGGGCCGTCCT
  GGCAGCCCGGCTGGCGTTTGTCATCGTCTTCCAGAACCTGGTCATGTTCATGAGCGACTTTGTGGACTGG
  GTCATCCCGGACATCCCCAAGGACATCAGCCAGCAGATCCACAAGGAGAAGGTGCTCATGGTGGAGCTGT
  TCATGCGGGAGGAGCAAGACAAGCAGCAGCTGCTGGAAACCTGGATGGAGAAGGAGCGGCAGAAGGACGA
  GCCGCCGTGCAACCACCACAACACCAAAGCCTGCCCAGACAGCCTCGGCAGCCCAGCCCCCAGCCATGCC
  TACCACGGGGGCGTCCTGTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_018043
• ORF Size: 2961 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_018043.5, NP_060513.5
• RefSeq Size: 4811
• RefSeq ORF: 2961
• Locus ID: 55107
• Domains: DUF590
• Protein Families: Transmembrane
• Gene Summary: Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. [UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.