PMPCA (NM_001282944) Human Tagged ORF Clone

CAT#: RC237948

PMPCA (myc-DDK-tagged) - Human peptidase (mitochondrial processing) alpha (PMPCA), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PMPCA
• Synonyms: Alpha-MPP; CLA1; CPD3; INPP5E; P-55; SCAR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237948 representing NM_001282944
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGAAATACCTTAGTGGAATTGCTCACTTTTTGGAAAAATTGGCATTTTCGTCTACTGCTCGATT
  TGACAGCAAAGATGAAATTCTGCTTACGTTGGAAAAGCATGGGGGTATCTGTGACTGCCAGACATCAAGA
  TGAAGAAGTCGAGATGACGCGGATGGCGGTCCAGTTTGAGCTGGAGGACCTGAACCTGCGGCCTGACCCA
  GAGCCACTTCTCACCGAGATGATTCATGAAGCGGCTTACAGGGAGAACACAGTTGGCCTCCACCGTTTCT
  GCCCCACAGAAAACGTAGCAAAGATCAACCGAGAGGTGCTGCATTCCTACCTGAGGAACTACTACACTCC
  CGACCGCATGGTGCTGGCCGGCGTGGGCGTGGAGCACGAGCATCTGGTGGACTGTGCCCGGAAGTACCTC
  CTGGGGGTCCAGCCGGCCTGGGGGAGCGCAGAGGCCGTGGATATTGACAGATCTGTGGCCCAGTACACTG
  GGGGGATTGCCAAGCTAGAAAGAGACATGTCCAATGTCAGCCTGGGCCCGACCCCCATCCCCGAGCTCAC
  GCACATCATGGTTGGACTGGAGAGCTGCTCCTTCCTGGAGGAGGACTTCATCCCCTTTGCAGTGTTGAAC
  ATGATGATGGGCGGAGGTGGCTCCTTCTCGGCTGGTGGGCCCGGCAAGGGCATGTTCTCCAGGCTCTACC
  TCAACGTGCTCAACAGGCACCACTGGATGTATAACGCGACCTCCTACCACCACAGCTACGAGGACACTGG
  CCTCCTTTGCATCCATGCCAGCGCCGACCCAAGACAGGTTCGAGAAATGGTAGAAATCATCACAAAGGAG
  TTTATTTTAATGGGCGGAACCGTGGACACGGTGGAGCTGGAACGAGCCAAGACGCAGCTGACATCAATGC
  TCATGATGAACCTGGAATCCAGGCCTGTGATCTTCGAGGATGTGGGGAGGCAGGTGCTGGCCACTCGCTC
  CAGAAAGCTGCCGCACGAGCTGTGCACGCTCATCCGCAACGTGAAGCCGGAAGATGTGAAGAGAGTCGCT
  TCTAAGATGCTCCGAGGGAAGCCGGCAGTGGCCGCCCTGGGTGACCTGACTGACCTGCCCACGTATGAGC
  ACATCCAGACCGCCCTGTCGAGTAAGGACGGGCGCCTGCCCAGGACGTACCGGCTCTTCCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237948 representing NM_001282944
  Red=Cloning site Green=Tags(s)
  
  MKRNTLVELLTFWKNWHFRLLLDLTAKMKFCLRWKSMGVSVTARHQDEEVEMTRMAVQFELEDLNLRPDP
  EPLLTEMIHEAAYRENTVGLHRFCPTENVAKINREVLHSYLRNYYTPDRMVLAGVGVEHEHLVDCARKYL
  LGVQPAWGSAEAVDIDRSVAQYTGGIAKLERDMSNVSLGPTPIPELTHIMVGLESCSFLEEDFIPFAVLN
  MMMGGGGSFSAGGPGKGMFSRLYLNVLNRHHWMYNATSYHHSYEDTGLLCIHASADPRQVREMVEIITKE
  FILMGGTVDTVELERAKTQLTSMLMMNLESRPVIFEDVGRQVLATRSRKLPHELCTLIRNVKPEDVKRVA
  SKMLRGKPAVAALGDLTDLPTYEHIQTALSSKDGRLPRTYRLFR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282944
• ORF Size: 1182 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001282944.1, NP_001269873.1
• RefSeq Size: 2087
• RefSeq ORF: 1185
• Locus ID: 23203
• Protein Families: Druggable Genome, Protease
• MW: 45.3 kDa
• Gene Summary: The protein encoded by this gene is found in the mitochondrion, where it represents the alpha subunit of a proteolytic heterodimer. This heterodimer is responsible for cleaving the transit peptide from nuclear-encoded mitochondrial proteins. Defects in this gene are a cause of spinocerebellar ataxia, autosomal recessive 2. [provided by RefSeq, Mar 2016]