DGKH (NM_001297429) Human Tagged ORF Clone

CAT#: RC239785

DGKH (myc-DDK-tagged) - Human diacylglycerol kinase, eta (DGKH), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DGKH
• Synonyms: DGKeta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239785 representing NM_001297429
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAACATTTCTCAGGGATGCACAACTGGTACGCCTGCTCCCACGCCCGACCCACCTTCTGTAACGT
  GTGCAGAGAGAGTCTTTCTGGAGTCACCTCCCATGGCCTGTCCTGCGAAGGTACACACTGCCTGCAAAGA
  TTTATACCATCCAATATGTCCACTTGGTCAATGTAAAGTATCTATCATACCTCCAATTGCACTAAACAGC
  ACCGATTCCGATGGTTTCTGTAGAGCAACATTTTCGTTCTGTGTTAGTCCTCTATTGGTTTTTGTCAATT
  CTAAGAGTGGAGATAATCAGGGAGTAAAGTTCCTCCGTCGCTTTAAACAGTTGCTAAATCCGGCTCAGGT
  GTTTGATTTAATGAATGGAGGTCCTCATTTAGGTTTAAGATTATTTCAGAAGTTTGACAATTTCCGGATT
  CTTGTTTGTGGAGGCGATGGAAGTGTAGGTTGGGTTTTGTCAGAAATCGATAAGCTCAACTTGAATAAAC
  AGTGTCAGCTGGGAGTGTTGCCTTTGGGTACAGGAAATGACCTTGCCCGAGTTCTTGGCTGGGGAGGTTC
  ATATGACGATGACACCCAACTTCCTCAGATCCTAGAGAAACTGGAACGAGCCAGTACCAAAATGTTGGAC
  AGGTGGAGTATAATGACATATGAACTCAAATTGCCACCAAAAGCTTCCCTACTTCCAGGACCTCCAGAAG
  CATCTGAAGAATTTTATATGACGATTTATGAAGACTCAGTTGCAACGCATCTTACAAAAATCCTCAATTC
  TGATGAACATGCAGTGGTCATATCTTCTGCCAAGACGCTATGTGAAACTGTAAAGGACTTCGTTGCCAAA
  GTAGAAAAGACGTATGACAAAACCTTGGAAAATGCCGTTGTAGCTGATGCCGTGGCCAGTAAATGTTCAG
  TCCTAAACGAGAAGCTCGAACAACTGCTGCAGGCTTTGCACACAGATTCCCAGGCTGCGCCTGTTCTCCC
  TGGCCTCAGCCCTCTCATTGTGGAAGAAGATGCTGTGGAATCGTCCAGTGAAGAGTCCCTGGGTGAAAGC
  AAAGAGCAGCTTGGGGATGACGTTACAAAACCTTCCTCCCAGAAAGCCGTCAAACCAAGGGAAATCATGT
  TGCGGGCAAATAGTTTAAAGAAAGCAGTGAGGCAAGTCATTGAGGAAGCCGGAAAAGTTATGGATGACCC
  GACAGTTCACCCCTGTGAACCAGCTAATCAGTCCTCTGATTATGACAGCACAGAAACAGATGAATCTAAG
  GAGGAAGCTAAAGATGATGGTGCCAAAGAATCAATAACTGTTAAAACTGCACCTCGGTCTCCAGATGCCC
  GGGCAAGTTATGGCCATTCCCAAACTGATTCTGTCCCTGGTCCAGCTGTGGCAGCCAGCAAAGAAAACCT
  CCCTGTGCTCAATACCAGAATAATCTGCCCAGGTTTAAGAGCAGGACTGGCTGCCTCAATTGCTGGGAGT
  TCGATTATCAACAAAATGTTACTGGCAAACATTGATCCTTTTGGTGCCACGCCGTTTATTGACCCGGATC
  TAGATTCCGTAGATGGATATTCAGAAAAATGTGTCATGAACAATTACTTTGGGATTGGATTAGATGCAAA
  AATTTCATTAGAATTTAATAATAAAAGAGAGGAGCACCCTGAAAAATGCAGGAGCCGAACTAAAAACTTG
  ATGTGGTATGGAGTCCTTGGAACCCGGGAGTTATTACAGAGATCGTACAAGAATTTAGAACAAAGGGTTC
  AACTTGAGTGTGATGGGCAGTATATTCCTCTTCCCAGCTTGCAAGGCATAGCCGTGTTGAACATTCCCAG
  CTATGCTGGAGGCACTAACTTTTGGGGTGGAACTAAAGAGGATGATATATTTGCTGCACCATCCTTTGAT
  GACAAGATCCTGGAAGTTGTAGCAATATTTGATAGCATGCAAATGGCAGTTTCAAGGGTCATTAAACTGC
  AGCATCATCGAATAGCCCAGTGCCGTACAGTGAAAATCACTATATTTGGTGACGAAGGAGTCCCAGTGCA
  AGTGGATGGTGAAGCGTGGGTTCAGCCTCCAGGGATTATCAAAATTGTGCACAAAAACAGAGCACAAATG
  CTAACAAGGGACAGAGCCTTTGAGAGCACTCTGAAATCTTGGGAAGATAAGCAGAAGTGTGATTCTGGTA
  AACCAGTTCTCCGAACCCATTTGTACATCCATCACGCCATTGACTTGGCAACAGAAGAGGTGTCGCAGAT
  GCAGCTATGCTCCCAGGCTGCAGAGGAGCTCATTACTAGGATATGTGACGCAGCCACAATTCACTGTCTT
  TTGGAGCAAGAACTGGCCCATGCTGTGAATGCCTGCTCCCATGCCCTGAATAAAGCCAACCCAAGGTGCC
  CGGAGAGTCTTACAAGAGACACTGCCACTGAAATAGCCATCAATGTGAAGGCGCTGTATAATGAAACAGA
  ATCTTTGCTAGTTGGCAGGGTTCCTTTGCAGCTGGAATCGCCACATGAAGAGCGAGTATCCAATGCCTTA
  CACTCTGTGGAGGTGGAATTACAGAAACTGACAGAGATTCCTTGGCTTTATTATATCTTACACCCAAATG
  AGGATGAGGAACCTCCTATGGATTGCACCAAAAGGAACAACAGAAGCACCGTATTTCGAATAGTGCCAAA
  GTTTAAAAAGGAAAAGGTTCAGAAGCAGAAGACAAGTTCACAGCCTGGATCTGGGGATACCGAAAGTGGG
  TCATGTGAAGCGAATTCTCCAGGGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239785 representing NM_001297429
  Red=Cloning site Green=Tags(s)
  
  MWNISQGCTTGTPAPTPDPPSVTCAERVFLESPPMACPAKVHTACKDLYHPICPLGQCKVSIIPPIALNS
  TDSDGFCRATFSFCVSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDNFRI
  LVCGGDGSVGWVLSEIDKLNLNKQCQLGVLPLGTGNDLARVLGWGGSYDDDTQLPQILEKLERASTKMLD
  RWSIMTYELKLPPKASLLPGPPEASEEFYMTIYEDSVATHLTKILNSDEHAVVISSAKTLCETVKDFVAK
  VEKTYDKTLENAVVADAVASKCSVLNEKLEQLLQALHTDSQAAPVLPGLSPLIVEEDAVESSSEESLGES
  KEQLGDDVTKPSSQKAVKPREIMLRANSLKKAVRQVIEEAGKVMDDPTVHPCEPANQSSDYDSTETDESK
  EEAKDDGAKESITVKTAPRSPDARASYGHSQTDSVPGPAVAASKENLPVLNTRIICPGLRAGLAASIAGS
  SIINKMLLANIDPFGATPFIDPDLDSVDGYSEKCVMNNYFGIGLDAKISLEFNNKREEHPEKCRSRTKNL
  MWYGVLGTRELLQRSYKNLEQRVQLECDGQYIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDIFAAPSFD
  DKILEVVAIFDSMQMAVSRVIKLQHHRIAQCRTVKITIFGDEGVPVQVDGEAWVQPPGIIKIVHKNRAQM
  LTRDRAFESTLKSWEDKQKCDSGKPVLRTHLYIHHAIDLATEEVSQMQLCSQAAEELITRICDAATIHCL
  LEQELAHAVNACSHALNKANPRCPESLTRDTATEIAINVKALYNETESLLVGRVPLQLESPHEERVSNAL
  HSVEVELQKLTEIPWLYYILHPNEDEEPPMDCTKRNNRSTVFRIVPKFKKEKVQKQKTSSQPGSGDTESG
  SCEANSPGN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001297429
• ORF Size: 2757 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001297429.1, NP_001284358.1
• RefSeq Size: 16746
• RefSeq ORF: 2760
• Locus ID: 160851
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 101.5 kDa
• Gene Summary: This gene encodes a member of the diacylglycerol kinase (DGK) enzyme family. Members of this family are involved in regulating intracellular concentrations of diacylglycerol and phosphatidic acid. Variation in this gene has been associated with bipolar disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jul 2014]