LTK (NM_002344) Human Tagged ORF Clone

CAT#: RC600053

LTK (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens leukocyte receptor tyrosine kinase, transcript variant 1, Signal peptide (1-16) plus EC domain (17-424)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: DDK-His
• Symbol: LTK
• Synonyms: TYK1
• Vector: pCMV6-XL5-DDK-His
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC600053 representing leader sequence plus the extracellular domain region of NM_002344
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGTCGACTGGATCTGGTACCGAGGAGATCCGCCGCCG
  CGATCGCC
  
  ATGGGCTGCTGGGGACAGCTGCTGGTGTGGTTCGGAGCCGCGGGCGCCATTCTCTGCTCTAGCCCGGGGT
  CCCAGGAGACTTTTCTGCGGTCCTCGCCCCTGCCGCTGGCAAGTCCCAGCCCCCGGGACCCGAAAGTCAG
  CGCCCCGCCTAGTATCTTGGAGCCAGCCTCCCCGCTGAATTCTCCGGGCACCGAGGGGTCTTGGCTGTTT
  TCTACCTGCGGGGCCAGCGGCCGGCATGGGCCCACACAGACACAATGTGACGGGGCGTACGCGGGGACCA
  GCGTGGTGGTGACCGTGGGGGCCGCCGGGCAGCTGAGAGGCGTGCAGCTGTGGCGCGTGCCGGGCCCTGG
  CCAGTATCTGATCTCAGCCTACGGAGCCGCGGGCGGCAAAGGCGCCAAGAACCACCTGTCGCGGGCGCAT
  GGCGTCTTCGTCTCAGCAATCTTCTCCCTCGGTCTCGGGGAGTCGCTGTACATCCTGGTGGGGCAGCAGG
  GAGAGGACGCCTGTCCCGGAGGTAGCCCGGAGAGCCAGCTCGTCTGCCTCGGGGAGTCTCGAGCCGTTGA
  AGAGCACGCGGCGATGGATGGGAGCGAAGGGGTCCCGGGGTCGCGGCGCTGGGCGGGAGGTGGCGGGGGT
  GGCGGGGGCGCCACCTACGTTTTCCGGGTGCGCGCTGGCGAGCTGGAACCGTTGCTGGTGGCGGCCGGAG
  GCGGCGGTCGGGCCTACCTGAGGCCGCGGGACCGAGGCCGGACTCAGGCCTCCCCCGAGAAACTGGAGAA
  CCGCTCGGAGGCGCCCGGGAGCGGCGGGAGAGGCGGGGCGGCAGGTGGTGGGGGCGGCTGGACGTCGCGG
  GCTCCCTCTCCGCAGGCCGGCCGCTCACTGCAGGAGGGGGCGGAGGGCGGCCAGGGCTGCTCCGAGGCTT
  GGGCGACCCTTGGCTGGGCCGCGGCCGGCGGCTTCGGGGGCGGCGGCGGGGCCTGCACTGCGGGCGGAGG
  CGGCGGCGGCTACAGGGGGGGCGACGCTTCAGAGACTGACAACCTCTGGGCTGATGGGGAAGATGGAGTA
  TCCTTCATACACCCCAGCAGCGAGCTCTTCCTGCAGCCTCTGGCAGTCACCGAGAACCACGGAGAGGTAG
  AGATCCGAAGGCACCTCAACTGCAGTCACTGCCCTTTGAGAGACTGCCAATGGCAGGCAGAGCTCCAGCT
  GGCTGAATGCCTGTGCCCAGAAGGCATGGAGCTAGCTGTGGATAACGTCACCTGCATGGACCTGCACAAG
  CCCCCAGGCCCT
  
  ACGCGTTCAGGCGACTACAAGGATGACGACGATAAGGGATCTCATCATCACCATCACCATTAATGAGATC
  TGGTACCGATATCAAGCTTGTCGACTCTAGA
  Protein Sequence

  >RC600053 representing signal peptide plus the extracellular domain region of NM_002344
  Red=Cloning sites Green= DDK and 6XHIS Tags
  
  MGCWGQLLVWFGAAGAILCSSPGSQETFLRSSPLPLASPSPRDPKVSAPPSILEPASPLNSPGTEGSWLF
  STCGASGRHGPTQTQCDGAYAGTSVVVTVGAAGQLRGVQLWRVPGPGQYLISAYGAAGGKGAKNHLSRAH
  GVFVSAIFSLGLGESLYILVGQQGEDACPGGSPESQLVCLGESRAVEEHAAMDGSEGVPGSRRWAGGGGG
  GGGATYVFRVRAGELEPLLVAAGGGGRAYLRPRDRGRTQASPEKLENRSEAPGSGGRGGAAGGGGGWTSR
  APSPQAGRSLQEGAEGGQGCSEAWATLGWAAAGGFGGGGGACTAGGGGGGYRGGDASETDNLWADGEDGV
  SFIHPSSELFLQPLAVTENHGEVEIRRHLNCSHCPLRDCQWQAELQLAECLCPEGMELAVDNVTCMDLHK
  PPGP
  
  TRSGTRSGDYKDDDDKGSHHHHHH
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_002344
• ORF Size: 1272 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the extra cellular domain of the protein with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_002344.5, NP_002335.2
• RefSeq Size: 3143
• RefSeq ORF: 2595
• Locus ID: 4058
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• MW: 42.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the ros/insulin receptor family of tyrosine kinases. Tyrosine-specific phosphorylation of proteins is a key to the control of diverse pathways leading to cell growth and differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]