WHSC2 (NELFA) (NM_005663) Human Tagged ORF Clone

CAT#: RG201097

NELFA (GFP-tagged) - Human Wolf-Hirschhorn syndrome candidate 2 (WHSC2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NELFA
• Synonyms: NELF-A; P/OKcl.15; WHSC2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201097 representing NM_005663
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCATGCGGGAGAGCGACACGGGCCTGTGGCTGCACAACAAGCTGGGGGCCACGGACGAGCTGT
  GGGCGCCGCCCAGCATCGCGTCCCTGCTCACGGCCGCGGTCATCGACAACATCCGTCTCTGCTTCCATGG
  CCTCTCGTCGGCAGTGAAGCTCAAGTTGCTACTCGGGACGCTGCACCTCCCGCGCCGCACGGTGGACGAG
  ATGAAGGGCGCCCTAATGGAGATCATCCAGCTCGCCAGCCTCGACTCGGACCCCTGGGTGCTCATGGTCG
  CCGACATCTTGAAGTCCTTTCCGGACACAGGCTCGCTTAACCTGGAGCTGGAGGAGCAGAATCCCAACGT
  TCAGGATATTTTGGGAGAACTTAGAGAAAAGGTGGGTGAGTGTGAAGCGTCTGCCATGCTGCCACTGGAG
  TGCCAGTACTTGAACAAAAACGCCCTGACGACCCTCGCGGGACCCCTCACTCCCCCGGTGAAGCATTTTC
  AGTTAAAGCGGAAACCCAAGAGCGCCACGCTGCGGGCGGAGCTGCTGCAGAAGTCCACGGAGACCGCCCA
  GCAGTTGAAGCGGAGCGCCGGGGTGCCCTTCCACGCCAAGGGCCGGGGGCTGCTGCGGAAGATGGACACC
  ACCACCCCACTCAAAGGCATCCCGAAGCAGGCGCCCTTCAGAAGCCCCACGGCGCCCAGCGTCTTCAGCC
  CCACAGGGAACCGGACCCCCATCCCGCCTTCCAGGACGCTGCTGCGGAAGGAACGAGGTGTGAAGCTGCT
  GGACATCTCTGAGCTGGATATGGTTGGCGCTGGCCGAGAGGCGAAGCGGAGAAGGAAGACTCTCGATGCG
  GAGGTGGTGGAGAAGCCGGCCAAGGAGGAAACGGTGGTGGAGAACGCCACCCCGGACTACGCAGCCGGCC
  TGGTGTCCACGCAGAAACTTGGGTCCCTGAACAATGAGCCTGCGCTGCCCTCCACGAGCTACCTTCCCTC
  CACGCCCAGCGTGGTTCCCGCCTCCTCCTACATCCCCAGCTCCGAGACACCCCCAGCCCCATCTTCCCGG
  GAAGCCAGCCGCCCACCAGAGGAGCCCAGCGCCCCGAGCCCCACGTTGCCAGCGCAGTTCAAGCAGCGGG
  CGCCCATGTACAACAGCGGCCTGAGCCCTGCCACACCCACGCCTGCGGCGCCCACCTCGCCTCTGACACC
  CACCACACCTCCGGCTGTCGCCCCTACCACTCAGACACCCCCGGTTGCCATGGTGGCCCCGCAGACCCAG
  GCCCCTGCTCAGCAGCAGCCTAAGAAGAACCTGTCCCTCACGAGAGAGCAGATGTTCGCTGCCCAGGAGA
  TGTTCAAGACGGCCAACAAAGTCACGCGGCCCGAGAAGGCCCTCATCCTGGGCTTCATGGCCGGCTCCCG
  AGAGAACCCGTGCCAGGAGCAGGGGGACGTGATCCAGATCAAGCTGAGCGAGCACACGGAGGACCTGCCC
  AAGGCGGACGGCCAGGGTAGCACAACCATGCTGGTGGACACAGTGTTTGAGATGAACTATGCCACGGGCC
  AGTGGACGCGCTTCAAGAAGTACAAGCCCATGACCAATGTGTCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201097 representing NM_005663
  Red=Cloning site Green=Tags(s)
  
  MASMRESDTGLWLHNKLGATDELWAPPSIASLLTAAVIDNIRLCFHGLSSAVKLKLLLGTLHLPRRTVDE
  MKGALMEIIQLASLDSDPWVLMVADILKSFPDTGSLNLELEEQNPNVQDILGELREKVGECEASAMLPLE
  CQYLNKNALTTLAGPLTPPVKHFQLKRKPKSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMDT
  TTPLKGIPKQAPFRSPTAPSVFSPTGNRTPIPPSRTLLRKERGVKLLDISELDMVGAGREAKRRRKTLDA
  EVVEKPAKEETVVENATPDYAAGLVSTQKLGSLNNEPALPSTSYLPSTPSVVPASSYIPSSETPPAPSSR
  EASRPPEEPSAPSPTLPAQFKQRAPMYNSGLSPATPTPAAPTSPLTPTTPPAVAPTTQTPPVAMVAPQTQ
  APAQQQPKKNLSLTREQMFAAQEMFKTANKVTRPEKALILGFMAGSRENPCQEQGDVIQIKLSEHTEDLP
  KADGQGSTTMLVDTVFEMNYATGQWTRFKKYKPMTNVS
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005663
• ORF Size: 1584 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_005663.2, NP_005654.2
• RefSeq Size: 2263
• RefSeq ORF: 1587
• Locus ID: 7469
• Protein Families: Transcription Factors
• Gene Summary: This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq, Jul 2008]