Asparagine synthetase (ASNS) (NM_001673) Human Tagged ORF Clone

CAT#: RG201297

ASNS (GFP-tagged) - Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ASNS
• Synonyms: ASNSD; TS11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201297 representing NM_001673
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGGCATTTGGGCGCTGTTTGGCAGTGATGATTGCCTTTCTGTTCAGTGTCTGAGTGCTATGAAGA
  TTGCACACAGAGGTCCAGATGCATTCCGTTTTGAGAATGTCAATGGATACACCAACTGCTGCTTTGGATT
  TCACCGGTTGGCGGTAGTTGACCCGCTGTTTGGAATGCAGCCAATTCGAGTGAAGAAATATCCGTATTTG
  TGGCTCTGTTACAATGGTGAAATCTACAACCATAAGAAGATGCAACAGCATTTTGAATTTGAATACCAGA
  CCAAAGTGGATGGTGAGATAATCCTTCATCTTTATGACAAAGGAGGAATTGAGCAAACAATTTGTATGTT
  GGATGGTGTGTTTGCATTTGTTTTACTGGATACTGCCAATAAGAAAGTGTTCCTGGGTAGAGATACATAT
  GGAGTCAGACCTTTGTTTAAAGCAATGACAGAAGATGGATTTTTGGCTGTATGTTCAGAAGCTAAAGGTC
  TTGTTACATTGAAGCACTCCGCGACTCCCTTTTTAAAAGTGGAGCCTTTTCTTCCTGGACACTATGAAGT
  TTTGGATTTAAAGCCAAATGGCAAAGTTGCATCCGTGGAAATGGTTAAATATCATCACTGTCGGGATGTA
  CCCCTGCACGCCCTCTATGACAATGTGGAGAAACTCTTTCCAGGTTTTGAGATAGAAACTGTGAAGAACA
  ACCTCAGGATCCTTTTTAATAATGCTGTAAAGAAACGTTTGATGACAGACAGAAGGATTGGCTGCCTTTT
  ATCAGGGGGCTTGGACTCCAGCTTGGTTGCTGCCACTCTGTTGAAGCAGCTGAAAGAAGCCCAAGTACAG
  TATCCTCTCCAGACATTTGCAATTGGCATGGAAGACAGCCCCGATTTACTGGCTGCTAGAAAGGTGGCAG
  ATCATATTGGAAGTGAACATTATGAAGTCCTTTTTAACTCTGAGGAAGGCATTCAGGCTCTGGATGAAGT
  CATATTTTCCTTGGAAACTTATGACATTACAACAGTTCGTGCTTCAGTAGGTATGTATTTAATTTCCAAG
  TATATTCGGAAGAACACAGATAGCGTGGTGATCTTCTCTGGAGAAGGATCAGATGAACTTACGCAGGGTT
  ACATATATTTTCACAAGGCTCCTTCTCCTGAAAAAGCCGAGGAGGAGAGTGAGAGGCTTCTGAGGGAACT
  CTATTTGTTTGATGTTCTCCGCGCAGATCGAACTACTGCTGCCCATGGTCTTGAACTGAGAGTCCCATTT
  CTAGATCATCGATTTTCTTCCTATTACTTGTCTCTGCCACCAGAAATGAGAATTCCAAAGAATGGGATAG
  AAAAACATCTCCTGAGAGAGACGTTTGAGGATTCCAATCTGATACCCAAAGAGATTCTCTGGCGACCAAA
  AGAAGCCTTCAGTGATGGAATAACTTCAGTTAAGAATTCCTGGTTTAAGATTTTACAGGAATACGTTGAA
  CATCAGGTTGATGATGCAATGATGGCAAATGCAGCCCAGAAATTTCCCTTCAATACTCCTAAAACCAAAG
  AAGGATATTACTACCGTCAAGTCTTTGAACGCCATTACCCAGGCCGGGCTGACTGGCTGAGCCATTACTG
  GATGCCCAAGTGGATCAATGCCACTGACCCTTCTGCCCGCACGCTGACCCACTACAAGTCAGCTGTCAAA
  GCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201297 representing NM_001673
  Red=Cloning site Green=Tags(s)
  
  MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVDPLFGMQPIRVKKYPYL
  WLCYNGEIYNHKKMQQHFEFEYQTKVDGEIILHLYDKGGIEQTICMLDGVFAFVLLDTANKKVFLGRDTY
  GVRPLFKAMTEDGFLAVCSEAKGLVTLKHSATPFLKVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDV
  PLHALYDNVEKLFPGFEIETVKNNLRILFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQLKEAQVQ
  YPLQTFAIGMEDSPDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLETYDITTVRASVGMYLISK
  YIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEEESERLLRELYLFDVLRADRTTAAHGLELRVPF
  LDHRFSSYYLSLPPEMRIPKNGIEKHLLRETFEDSNLIPKEILWRPKEAFSDGITSVKNSWFKILQEYVE
  HQVDDAMMANAAQKFPFNTPKTKEGYYYRQVFERHYPGRADWLSHYWMPKWINATDPSARTLTHYKSAVK
  A
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001673
• ORF Size: 1683 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001673.2, NP_001664.2
• RefSeq Size: 1994 bp
• RefSeq ORF: 1686 bp
• Locus ID: 440
• Cytogenetics: 7q21.3
• Domains: GATase_2, Asn_synthase
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways, Nitrogen metabolism
• Gene Summary: 'The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]'