Asparagine synthetase (ASNS) (NM_001673) Human Untagged Clone

CAT#: SC321218

ASNS (untagged)-Human asparagine synthetase (glutamine-hydrolyzing) (ASNS), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASNS
• Synonyms: ASNSD; TS11
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001673.2 GCCGCCCGTCAAGCTGTCCACATCCCTGGCCTCAGCCCGCCACATCACCCTGACCTGCTT
  ACGCCCAGATTTTCTTCAATCACATCTGAATAAATCACTTGAAGAAAGCTTATAGCTTCA
  TTGCACCATGTGTGGCATTTGGGCGCTGTTTGGCAGTGATGATTGCCTTTCTGTTCAGTG
  TCTGAGTGCTATGAAGATTGCACACAGAGGTCCAGATGCATTCCGTTTTGAGAATGTCAA
  TGGATACACCAACTGCTGCTTTGGATTTCACCGGTTGGCGGTAGTTGACCCGCTGTTTGG
  AATGCAGCCAATTCGAGTGAAGAAATATCCGTATTTGTGGCTCTGTTACAATGGTGAAAT
  CTACAACCATAAGAAGATGCAACAGCATTTTGAATTTGAATACCAGACCAAAGTGGATGG
  TGAGATAATCCTTCATCTTTATGACAAAGGAGGAATTGAGCAAACAATTTGTATGTTGGA
  TGGTGTGTTTGCATTTGTTTTACTGGATACTGCCAATAAGAAAGTGTTCCTGGGTAGAGA
  TACATATGGAGTCAGACCTTTGTTTAAAGCAATGACAGAAGATGGATTTTTGGCTGTATG
  TTCAGAAGCTAAAGGTCTTGTTACATTGAAGCACTCCGCGACTCCCTTTTTAAAAGTGGA
  GCCTTTTCTTCCTGGACACTATGAAGTTTTGGATTTAAAGCCAAATGGCAAAGTTGCATC
  CGTGGAAATGGTTAAATATCATCACTGTCGGGATGTACCCCTGCACGCCCTCTATGACAA
  TGTGGAGAAACTCTTTCCAGGTTTTGAGATAGAAACTGTGAAGAACAACCTCAGGATCCT
  TTTTAATAATGCTGTAAAGAAACGTTTGATGACAGACAGAAGGATTGGCTGCCTTTTATC
  AGGGGGCTTGGACTCCAGCTTGGTTGCTGCCACTCTGTTGAAGCAGCTGAAAGAAGCCCA
  AGTACAGTATCCTCTCCAGACATTTGCAATTGGCATGGAAGACAGCCCCGATTTACTGGC
  TGCTAGAAAGGTGGCAGATCATATTGGAAGTGAACATTATGAAGTCCTTTTTAACTCTGA
  GGAAGGCATTCAGGCTCTGGATGAAGTCATATTTTCCTTGGAAACTTATGACATTACAAC
  AGTTCGTGCTTCAGTAGGTATGTATTTAATTTCCAAGTATATTCGGAAGAACACAGATAG
  CGTGGTGATCTTCTCTGGAGAAGGATCAGATGAACTTACGCAGGGTTACATATATTTTCA
  CAAGGCTCCTTCTCCTGAAAAAGCCGAGGAGGAGAGTGAGAGGCTTCTGAGGGAACTCTA
  TTTGTTTGATGTTCTCCGCGCAGATCGAACTACTGCTGCCCATGGTCTTGAACTGAGAGT
  CCCATTTCTAGATCATCGATTTTCTTCCTATTACTTGTCTCTGCCACCAGAAATGAGAAT
  TCCAAAGAATGGGATAGAAAAACATCTCCTGAGAGAGACGTTTGAGGATTCCAATCTGAT
  ACCCAAAGAGATTCTCTGGCGACCAAAAGAAGCCTTCAGTGATGGAATAACTTCAGTTAA
  GAATTCCTGGTTTAAGATTTTACAGGAATACGTTGAACATCAGGTTGATGATGCAATGAT
  GGCAAATGCAGCCCAGAAATTTCCCTTCAATACTCCTAAAACCAAAGAAGGATATTACTA
  CCGTCAAGTCTTTGAACGCCATTACCCAGGCCGGGCTGACTGGCTGAGCCATTACTGGAT
  GCCCAAGTGGATCAATGCCACTGACCCTTCTGCCCGCACGCTGACCCACTACAAGTCAGC
  TGTCAAAGCTTAGGTGGTCTTTATGCTGTAATGTGAAAGCAAATATTTCTTCGTGTTGGA
  TGGGGACTGTGGGTAGATAGGGGAACAATGAGAGTCAACTCAGGCTAACTTGGGTGTGAA
  AAAAATAAAAGTCCTAAATCTAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001673
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001673.2, NP_001664.2
• RefSeq Size: 1994 bp
• RefSeq ORF: 1686 bp
• Locus ID: 440
• Cytogenetics: 7q21.3
• Domains: GATase_2, Asn_synthase
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways, Nitrogen metabolism
• Gene Summary: 'The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3, and 7 encode the same isoform (a).