MTGR1 (CBFA2T2) (NM_001039709) Human Tagged ORF Clone

CAT#: RG202013

CBFA2T2 (GFP-tagged) - Human core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CBFA2T2
• Synonyms: EHT; MTGR1; p85; ZMYND3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202013 representing NM_001039709
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTTTCACCATGTTGGCCAGGCTCGTCTTGAACTCCTGACCTCAGGTGATCTGCCTGCATTGGCCT
  CCCAACGTGCTGGGATTACAGTTGGTCCTGAGAAAAGGGTGCCAGCGATGCCTGGATCGCCTGTGGAAGT
  GAAGATACAGTCCAGATCCTCACCTCCCACCATGCCACCCCTCCCACCAATAAATCCTGGAGGACCGAGG
  CCAGTGTCCTTCACTCCTACTGCATTAAGCAATGGCATCAACCATTCTCCTCCTACCCTGAATGGTGCCC
  CATCACCGCCACAGAGATTCAGCAATGGTCCTGCCTCCTCCACATCATCTGCACTCACAAATCAGCAATT
  GCCAGCCACTTGTGGTGCTCGACAACTCAGCAAGTTGAAACGCTTTCTTACCACTCTGCAACAGTTTGGC
  AATGACATCTCCCCTGAGATTGGGGAGAAGGTGCGGACTCTTGTTCTTGCACTGGTGAACTCAACAGTGA
  CAATTGAGGAATTCCACTGTAAGCTCCAAGAAGCCACAAACTTTCCCCTTCGTCCTTTTGTGATTCCATT
  TCTCAAGGCCAACCTGCCCCTGCTGCAGCGGGAACTGCTGCACTGCGCTCGGGCGGCCAAGCAGACCCCA
  TCCCAGTACCTGGCTCAGCACGAACACCTTCTGCTCAACACAAGCATTGCATCGCCTGCTGACTCGTCAG
  AGTTGCTCATGGAGGTGCACGGAAATGGGAAGAGGCCCAGTCCAGAGAGGAGAGAAGAGAATAGTTTTGA
  TAGAGACACAATTGCTCCTGAGCCTCCTGCCAAGAGAGTATGTACCATCAGCCCTGCTCCTCGGCACAGT
  CCTGCTCTCACTGTGCCCCTCATGAATCCTGGGGGCCAATTCCATCCTACCCCTCCACCTCTTCAGCATT
  ACACCTTAGAGGATATTGCAACTTCTCACCTGTATCGGGAACCCAACAAGATGCTAGAGCATCGAGAAGT
  TCGTGATAGACACCACAGTCTTGGTCTAAATGGAGGCTATCAAGATGAGTTGGTAGATCATCGTTTGACA
  GAAAGGGAATGGGCTGATGAATGGAAACATCTTGACCATGCGCTGAATTGCATTATGGAAATGGTAGAGA
  AAACAAGGCGCTCTATGGCAGTTCTGCGGCGCTGTCAGGAATCAGATCGTGAAGAACTCAACTACTGGAA
  AAGACGGTACAATGAAAACACAGAGCTGAGGAAAACGGGGACCGAGTTGGTCTCCAGGCAGCACAGCCCT
  GGGAGTGCAGATTCTCTCAGCAATGATTCTCAGAGAGAGTTCAACAGCAGGCCAGGTACAGGATACGTAC
  CTGTGGAGTTTTGGAAAAAAACAGAAGAAGCTGTGAATAAGGTGAAAATTCAGGCCATGTCAGAAGTACA
  GAAGGCCGTCGCTGAGGCAGAGCAGAAAGCCTTTGAAGTGATTGCAACAGAGAGAGCACGAATGGAGCAA
  ACCATAGCGGATGTCAAGCGGCAGGCCGCAGAGGATGCTTTCCTCGTCATCAATGAGCAAGAGGAGTCCA
  CGGAGAACTGCTGGAACTGTGGCCGCAAAGCCAGCGAGACATGCAGTGGCTGCAATATCGCGCGATACTG
  TGGCTCTTTCTGCCAGCACAAGGACTGGGAGCGGCACCACCGCCTCTGTGGTCAGAACCTGCATGGCCAG
  AGCCCCCACGGCCAGGGCCGGCCGCTGCTTCCTGTAGGCAGGGGCTCCTCTGCCAGGTCCGCCGACTGCA
  GCGTGCCCAGCCCAGCCCTCGACAAGACCTCGGCAACCACATCGCGTTCCTCAACACCTGCTTCTGTGAC
  AGCTATCGACACCAACGGACTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202013 representing NM_001039709
  Red=Cloning site Green=Tags(s)
  
  MGFHHVGQARLELLTSGDLPALASQRAGITVGPEKRVPAMPGSPVEVKIQSRSSPPTMPPLPPINPGGPR
  PVSFTPTALSNGINHSPPTLNGAPSPPQRFSNGPASSTSSALTNQQLPATCGARQLSKLKRFLTTLQQFG
  NDISPEIGEKVRTLVLALVNSTVTIEEFHCKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARAAKQTP
  SQYLAQHEHLLLNTSIASPADSSELLMEVHGNGKRPSPERREENSFDRDTIAPEPPAKRVCTISPAPRHS
  PALTVPLMNPGGQFHPTPPPLQHYTLEDIATSHLYREPNKMLEHREVRDRHHSLGLNGGYQDELVDHRLT
  EREWADEWKHLDHALNCIMEMVEKTRRSMAVLRRCQESDREELNYWKRRYNENTELRKTGTELVSRQHSP
  GSADSLSNDSQREFNSRPGTGYVPVEFWKKTEEAVNKVKIQAMSEVQKAVAEAEQKAFEVIATERARMEQ
  TIADVKRQAAEDAFLVINEQEESTENCWNCGRKASETCSGCNIARYCGSFCQHKDWERHHRLCGQNLHGQ
  SPHGQGRPLLPVGRGSSARSADCSVPSPALDKTSATTSRSSTPASVTAIDTNGL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039709
• ORF Size: 1842 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001039709.1, NP_001034798.1
• RefSeq Size: 7449
• RefSeq ORF: 1728
• Locus ID: 9139
• Protein Families: Transcription Factors
• Gene Summary: In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. [provided by RefSeq, Jul 2008]