TKTL1 (NM_012253) Human Tagged ORF Clone

CAT#: RG205218

TKTL1 (GFP-tagged) - Human transketolase-like 1 (TKTL1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TKTL1
• Synonyms: TKR; TKT2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205218 representing NM_012253
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATGCTGAGGCGAGGGCTGAGTTCCCGGAGGAGGCCAGACCTGACAGGGGCACCTTGCAGGTGT
  TTCAAGATATGGCCAGCCGCTTGCGAATCCATTCCATCAGGGCCACATGCTCCACGAGCTCCGGCCACCC
  TACATCATGTAGCAGTTCTTCTGAGATCATGTCTGTGCTGTTCTTCTACATCATGAGGTACAAGCAGTCA
  GATCCAGAGAATCCGGACAACGACCGATTTGTCCTCGCAAAGAGACTGTCGTTTGTGGATGTGGCAACAG
  GATGGCTCGGACAAGGACTGGGAGTTGCATGTGGAATGGCATATACTGGCAAGTACTTCGACAGGGCCAG
  CTACCGGGTGTTCTGCCTCATGAGTGATGGCGAGTCCTCAGAAGGCTCTGTCTGGGAGGCAATGGCCTTT
  GCTTCCTACTACAGTCTGGACAATCTTGTGGCAACCTTTGATGTGAACCGCCTGGGACACAGTGGTGCAT
  TGCCCGCCGAGCACTGCATAAACATCTATCAGAGGCGCTGCGAAGCCTTTGGGTGGAACACTTATGTGGT
  GGACGGCCGGGACGTGGAGGCACTGTGCCAGGTATTCTGGCAGGCTTCTCAGGTGAAGCACAAGCCCACT
  GCTGTGGTGGCCAAGACCTTCAAGGGCCGGGGCACCCCAAGTATTGAGGATGCAGAAAGTTGGCATGCAA
  AGCCAATGCCGAGAGAAAGAGCAGATGCCATTATCAAATTAATTGAGAGCCAGATACAGACCAGCAGGAA
  TCTTGACCCACAGCCCCCCATTGAGGACTCACCTGAAGTCAACATCACAGATGTAAGGATGACCTCTCCA
  CCTGATTACAGAGTTGGTGACAAGATAGCTACTCGGAAAGCATGCGGTCTGGCTCTGGCTAAGCTGGGCT
  ACGCGAACAACAGAGTCGTTGTGCTGGATGGTGACACCAGGTACTCTACTTTCTCTGAGATATTCAACAA
  GGAGTACCCTGAGCGCTTCATCGAGTGCTTTATGGCTGAACAAAACATGGTGAGCGTGGCTCTGGGCTGT
  GCCTCCCGTGGACGGACCATTGCTTTTGCTAGCACCTTTGCTGCCTTTCTGACTCGAGCATTTGATCACA
  TCCGGATAGGAGGCCTCGCTGAGAGCAACATCAACATTATTGGTTCCCACTGTGGGGTATCTGTTGGTGA
  CGATGGTGCTTCCCAGATGGCCCTGGAGGATATAGCCATGTTCCGAACCATTCCCAAGTGCACGATCTTC
  TACCCAACTGATGCCGTCTCCACGGAGCATGCTGTTGCTCTGGCAGCCAATGCCAAGGGGATGTGCTTCA
  TTCGGACCACCCGACCAGAAACTATGGTTATTTACACCCCACAAGAACGCTTTGAGATCGGACAGGCCAA
  GGTCCTCCGCCACTGTGTCAGTGACAAGGTCACAGTTATTGGAGCTGGAATTACTGTGTATGAAGCCTTA
  GCAGCTGCTGATGAGCTTTCGAAACAAGATATTTTTATCCGTGTCATCGACCTGTTTACCATTAAACCTC
  TGGATGTCGCCACCATCGTCTCCAGTGCAAAAGCCACAGAGGGCCGGATCATTACAGTGGAGGATCACTA
  CCCGCAAGGTGGCATCGGGGAAGCTGTCTGCGCAGCCGTCTCCATGGATCCTGACATTCAGGTTCATTCG
  CTGGCAGTGTCGGGAGTGCCCCAGAGTGGGAAGTCCGAGGAATTGCTGGATATGTATGGAATTAGTGCCA
  GACATATCATAGTGGCCGTGAAATGCATGTTGCTGAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205218 representing NM_012253
  Red=Cloning site Green=Tags(s)
  
  MADAEARAEFPEEARPDRGTLQVFQDMASRLRIHSIRATCSTSSGHPTSCSSSSEIMSVLFFYIMRYKQS
  DPENPDNDRFVLAKRLSFVDVATGWLGQGLGVACGMAYTGKYFDRASYRVFCLMSDGESSEGSVWEAMAF
  ASYYSLDNLVATFDVNRLGHSGALPAEHCINIYQRRCEAFGWNTYVVDGRDVEALCQVFWQASQVKHKPT
  AVVAKTFKGRGTPSIEDAESWHAKPMPRERADAIIKLIESQIQTSRNLDPQPPIEDSPEVNITDVRMTSP
  PDYRVGDKIATRKACGLALAKLGYANNRVVVLDGDTRYSTFSEIFNKEYPERFIECFMAEQNMVSVALGC
  ASRGRTIAFASTFAAFLTRAFDHIRIGGLAESNINIIGSHCGVSVGDDGASQMALEDIAMFRTIPKCTIF
  YPTDAVSTEHAVALAANAKGMCFIRTTRPETMVIYTPQERFEIGQAKVLRHCVSDKVTVIGAGITVYEAL
  AAADELSKQDIFIRVIDLFTIKPLDVATIVSSAKATEGRIITVEDHYPQGGIGEAVCAAVSMDPDIQVHS
  LAVSGVPQSGKSEELLDMYGISARHIIVAVKCMLLN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012253
• ORF Size: 1788 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012253.2, NP_036385.2
• RefSeq Size: 2530
• RefSeq ORF: 1791
• Locus ID: 8277
• Domains: transketolase, transket_pyr, transketolase_C
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pentose phosphate pathway
• Gene Summary: The protein encoded by this gene is a transketolase that acts as a homodimer and catalyzes the conversion of sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate to D-ribose 5-phosphate and D-xylulose 5-phosphate. This reaction links the pentose phosphate pathway with the glycolytic pathway. Variations in this gene may be the cause of Wernicke-Korsakoff syndrome. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]