DUSP10 (NM_007207) Human Tagged ORF Clone

CAT#: RG206760

DUSP10 (GFP-tagged) - Human dual specificity phosphatase 10 (DUSP10), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DUSP10
• Synonyms: MKP-5; MKP5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206760 representing NM_007207
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCCGTCTCCTTTAGACGACAGGGTAGTAGTGGCACTATCTAGGCCCGTCCGACCTCAGGATCTCA
  ACCTTTGTTTAGACTCTAGTTACCTTGGCTCTGCCAACCCAGGCAGTAACAGCCACCCTCCTGTCATCGC
  CACCACCGTTGTGTCCCTCAAGGCTGCGAATCTGACGTATATGCCCTCATCCAGCGGCTCTGCCCGCTCG
  CTGAATTGTGGATGCAGCAGTGCCAGCTGCTGCACTGTGGCAACCTACGACAAGGACAATCAGGCCCAAA
  CCCAAGCCATTGCCGCTGGCACCACCACCACTGCCATCGGAACCTCTACCACCTGCCCTGCTAACCAGAT
  GGTCAACAATAATGAGAATACAGGCTCTCTAAGTCCATCAAGTGGGGTGGGCAGCCCTGTGTCAGGGACC
  CCCAAGCAGCTAGCCAGCATCAAAATAATCTACCCCAATGACTTGGCAAAGAAGATGACCAAATGCAGCA
  AGAGTCACCTGCCGAGTCAGGGCCCTGTCATCATTGACTGCAGGCCCTTCATGGAGTACAACAAGAGTCA
  CATCCAAGGAGCTGTCCACATTAACTGTGCCGATAAGATCAGCCGGCGGAGACTGCAGCAGGGCAAGATC
  ACTGTCCTAGACTTGATTTCCTGTAGGGAAGGCAAGGACTCTTTCAAGAGGATCTTTTCCAAAGAAATTA
  TAGTTTATGATGAGAATACCAATGAACCAAGCCGAGTGATGCCCTCCCAGCCACTTCACATAGTCCTCGA
  GTCCCTGAAGAGAGAAGGCAAAGAACCTCTGGTGTTGAAAGGTGGACTTAGTAGTTTTAAGCAGAACCAT
  GAAAACCTCTGTGACAACTCCCTCCAGCTCCAAGAGTGCCGGGAGGTGGGGGGCGGCGCATCCGCGGCCT
  CGAGCTTGCTACCTCAGCCCATCCCCACCACCCCTGACATCGAGAACGCTGAGCTCACCCCCATCTTGCC
  CTTCCTGTTCCTTGGCAATGAGCAGGATGCTCAGGACCTGGACACCATGCAGCGGCTGAACATCGGCTAC
  GTCATCAACGTCACCACTCATCTTCCCCTCTACCACTATGAGAAAGGCCTGTTCAACTACAAGCGGCTGC
  CAGCCACTGACAGCAACAAGCAGAACCTGCGGCAGTACTTTGAAGAGGCTTTTGAGTTCATTGAGGAAGC
  TCACCAGTGTGGGAAGGGGCTTCTCATCCACTGCCAGGCTGGGGTGTCCCGCTCCGCCACCATCGTCATC
  GCTTACTTGATGAAGCACACTCGGATGACCATGACTGATGCTTATAAATTTGTCAAAGGCAAACGACCAA
  TTATCTCCCCAAACCTTAACTTCATGGGGCAGTTGCTAGAGTTCGAGGAAGACCTAAACAACGGTGTGAC
  ACCGAGAATCCTTACACCAAAGCTGATGGGCGTGGAGACGGTTGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206760 representing NM_007207
  Red=Cloning site Green=Tags(s)
  
  MPPSPLDDRVVVALSRPVRPQDLNLCLDSSYLGSANPGSNSHPPVIATTVVSLKAANLTYMPSSSGSARS
  LNCGCSSASCCTVATYDKDNQAQTQAIAAGTTTTAIGTSTTCPANQMVNNNENTGSLSPSSGVGSPVSGT
  PKQLASIKIIYPNDLAKKMTKCSKSHLPSQGPVIIDCRPFMEYNKSHIQGAVHINCADKISRRRLQQGKI
  TVLDLISCREGKDSFKRIFSKEIIVYDENTNEPSRVMPSQPLHIVLESLKREGKEPLVLKGGLSSFKQNH
  ENLCDNSLQLQECREVGGGASAASSLLPQPIPTTPDIENAELTPILPFLFLGNEQDAQDLDTMQRLNIGY
  VINVTTHLPLYHYEKGLFNYKRLPATDSNKQNLRQYFEEAFEFIEEAHQCGKGLLIHCQAGVSRSATIVI
  AYLMKHTRMTMTDAYKFVKGKRPIISPNLNFMGQLLEFEEDLNNGVTPRILTPKLMGVETVV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007207
• ORF Size: 1446 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007207.3, NP_009138.1
• RefSeq Size: 2619
• RefSeq ORF: 1449
• Locus ID: 11221
• Domains: DSPc, RHOD
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAP kinase superfamily, which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAP kinases, different tissue distribution and subcellular localization, and different modes of expression induction by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]