DACH1 (NM_080760) Human Tagged ORF Clone

CAT#: RG219215

DACH1 (GFP-tagged) - Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DACH1
• Synonyms: DACH
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219215 representing NM_080760
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTGCCGGCGGCTTTGATCCCTCCGACCCAGCTGGTCCCCCCTCAACCCCCAATCTCCACGTCTG
  CTTCCTCCTCTGGCACCACCACCTCCACCTCTTCGGCGACTTCGTCTCCGGCTCCTTCCATCGGACCCCC
  GGCGTCCTCTGGGCCAACTCTGTTCCGCCCGGAGCCCATCGCTTCGGCGGCGGCGGCGGCGGCCACAGTC
  ACCTCTACCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCAGCGGAGGCGGCGGCGGCAGCAGCGGCAACG
  GAGGCGGCGGTGGCGGCGGCGGCGGTGGCAGCAACTGCAACCCCAACCTGGCGGCCGCGAGCAACGGCAG
  CGGCGGCGGCGGCGGCGGCATCAGCGCTGGCGGCGGCGTCGCTTCCAGCACCCCCATCAACGCCAGCACC
  GGCAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGCAGTAGTAGCAGCAGCAGCAGTAGCAGCA
  GCAGCTGCGGCCCCCTCCCCGGGAAACCCGTGTACTCAACCCCGTCCCCAGTGGAAAACACCCCTCAGAA
  TAATGAGTGCAAAATGGTGGATCTGAGGGGGGCCAAAGTGGCTTCCTTCACGGTGGAGGGCTGCGAGCTG
  ATCTGCCTGCCCCAGGCTTTCGACCTGTTCCTGAAGCACTTGGTGGGGGGCTTGCATACGGTCTACACCA
  AGCTGAAGCGGCTGGAGATCACGCCGGTGGTGTGCAATGTGGAACAAGTTCGCATCCTGAGGGGACTGGG
  CGCCATCCAGCCAGGAGTGAACCGCTGCAAACTCATCTCCAGGAAGGACTTCGAGACCCTCTACAATGAC
  TGCACCAACGCAAGTTCTAGACCTGGAAGGCCTCCTAAGAGGACTCAAAGTGTCACCTCCCCAGAGAACT
  CTCACATCATGCCGCATTCTGTCCCTGGTCTCATGTCTCCTGGGATAATTCCACCAACAGGTCTGACAGC
  AGCCGCTGCAGCAGCTGCTGCTGCTACCAATGCAGCTATTGCTGAAGCAATGAAGGTGAAAAAAATCAAA
  TTAGAAGCCATGAGCAACTATCATGCCAGTAATAACCAACATGGAGCAGACTCTGAAAACGGGGACATGA
  ATTCAAGTGTCGATGAGACCCCGCTTTCTACACCAACCGCAAGAGACAGCCTTGACAAACTCTCTCTAAC
  TGGGCATGGACAACCACTGCCTCCAGGTTTTCCATCTCCTTTTCTGTTTCCTGATGGACTGTCTTCCATC
  GAGACTCTTCTGACTAACATACAGGGGCTGTTGAAAGTTGCCATAGATAATGCCAGAGCTCAAGAGAAAC
  AGGTCCAACTGGAAAAAACTGAGCTGAAGATGGATTTTTTAAGGGAAAGAGAACTAAGGGAAACACTTGA
  GAAGCAGTTGGCTATGGAACAAAAGAATAGAGCCATAGTTCAAAAGAGGCTAAAGAAGGAGAAGAAGGCA
  AAGAGAAAATTGCAGGAAGCACTTGAGTTTGAGACGAAACGGCGTGAACAAGCAGAACAGACGCTAAAAC
  AGGCAGCTTCAACAGATAGTCTCAGGGTCTTAAATGACTCTCTGACCCCAGAGATAGAGGCTGACCGCAG
  TGGCGGCAGAACAGATGCTGAAAGGACAATACAAGATGGAAGACTGTATTTGAAAACTACTGTCATGTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219215 representing NM_080760
  Red=Cloning site Green=Tags(s)
  
  MAVPAALIPPTQLVPPQPPISTSASSSGTTTSTSSATSSPAPSIGPPASSGPTLFRPEPIASAAAAAATV
  TSTGGGGGGGGGGSGGGGGSSGNGGGGGGGGGGSNCNPNLAAASNGSGGGGGGISAGGGVASSTPINAST
  GSSSSSSSSSSSSSSSSSSSSSSSSCGPLPGKPVYSTPSPVENTPQNNECKMVDLRGAKVASFTVEGCEL
  ICLPQAFDLFLKHLVGGLHTVYTKLKRLEITPVVCNVEQVRILRGLGAIQPGVNRCKLISRKDFETLYND
  CTNASSRPGRPPKRTQSVTSPENSHIMPHSVPGLMSPGIIPPTGLTAAAAAAAAATNAAIAEAMKVKKIK
  LEAMSNYHASNNQHGADSENGDMNSSVDETPLSTPTARDSLDKLSLTGHGQPLPPGFPSPFLFPDGLSSI
  ETLLTNIQGLLKVAIDNARAQEKQVQLEKTELKMDFLRERELRETLEKQLAMEQKNRAIVQKRLKKEKKA
  KRKLQEALEFETKRREQAEQTLKQAASTDSLRVLNDSLTPEIEADRSGGRTDAERTIQDGRLYLKTTVMY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080760
• ORF Size: 1680 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080760.4, NP_542938.2
• RefSeq Size: 4802 bp
• RefSeq ORF: 1677 bp
• Locus ID: 1602
• Cytogenetics: 13q21.33
• Domains: Ski_Sno
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a chromatin-associated protein that associates with other DNA-binding transcription factors to regulate gene expression and cell fate determination during development. The protein contains a Ski domain that is highly conserved from Drosophila to human. Expression of this gene is lost in some forms of metastatic cancer, and is correlated with poor prognosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]'