DACH1 (NM_080760) Human Untagged Clone

CAT#: SC313997

DACH1 (untagged)-Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DACH1
• Synonyms: DACH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC313997 sequence for NM_080760 edited (data generated by NextGen Sequencing)
  
  
  ATGGCAGTGCCGGCGGCTTTGATCCCTCCGACCCAGCTGGTCCCCCCTCAACCCCCAATCTCCACGTCTG
  CTTCCTCCTCTGGCACCACCACCTCCACCTCTTCGGCGACTTCGTCTCCGGCTCCTTCCATCGGACCCCC
  GGCGTCCTCTGGGCCAACTCTGTTCCGCCCGGAGCCCATCGCTTCGGCGGCGGCGGCGGCGGCCACAGTC
  ACCTCTACCGGCGGCGGCGGCGGCGGCGGCGGCAGCGGAGGCGGCGGCGGCAGCAGCGGCAACGGAGGCG
  GCGGTGGCGGCGGCGGCGGTGGCAGCAACTGCAACCCCAACCTGGCGGCCGCGAGCAACGGCAGCGGCGG
  CGGCGGCGGCGGCATCAGCGCTGGCGGCGGCGTCGCTTCCAGCACCCCCATCAACGCCAGCACCGGCAGC
  AGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGCAGTAGTAGCAGCAGCAGCAGTAGCAGCAGCAGCT
  GCGGCCCCCTCCCCGGGAAACCCGTGTACTCAACCCCGTCCCCAGTGGAAAACACCCCTCAGAATAATGA
  GTGCAAAATGGTGGATCTGAGGGGGGCCAAAGTGGTTTCCTTCACGGTGGAGGGCTGCGAGCTGATCTGC
  CTGCCCCAGGCTTTCGACCTGTTCCTGAAGCACTTGGTGGGGGGCTTGCATACGGTCTACACCAAGCTGA
  AGCGGCTGGAGATCACGCCGGTGGTGTGCAATGTGGAACAAGTTCGCATCCTGAGGGGACTGGGCGCCAT
  CCAGCCAGGAGTGAACCGCTGCAAACTCATCTCCAGGAAGGACTTCGAGACCCTCTACAATGACTGCACC
  AACGCAAGTTCTAGACCTGGAAGGCCTCCTAAGAGGACTCAAAGTGTCACCTCCCCAGAGAACTCTCACA
  TCATGCCGCATTCTGTCCCTGGTCTCATGTCTCCTGGGATAATTCCACCAACAGGTCTGACAGCAGCCGC
  TGCAGCAGCTGCTGCTGCTACCAATGCAGCTATTGCTGAAGCAATGAAGGTGAAAAAAATCAAATTAGAA
  GCCATGAGCAACTATCATGCCAGTAATAACCAACATGGAGCAGACTCTGAAAACGGGGACATTTTTTTAA
  TTTTAGTTGTTACACCGAGTTCTACACCAACCGCAAGAGACAGCCTTGACAAACTCTCTCTAACTGGGCA
  TGGACAACCACTGCCTCCAGGTTTTCCATCTCCTTTTCTGTTTCCTGATGGACTGTCTTCCATCGAGACT
  CTTCTGACTAACATACAGGGGCTGTTGAAAGTTGCCATAGATAATGCCAGAGCTCAAGAGAAACAGGTCC
  AACTGGAAAAAACTGAGCTGAAGATGGATTTTTTAAGGGAAAGAGAACTAAGGGAAACACTTGAGAAGCA
  GTTGGCTATGGAACAAAAGAATAGAGCCATAGTTCAAAAGAGGCTAAAGAAGGAGAAGAAGGCAAAGAGA
  AAATTGCAGGAAGCACTTGAGTTTGAGACGAAACGGCGTGAACAAGCAGAACAGACGCTAAAACAGGCAG
  CTTCAACAGATAGTCTCAGGGTCTTAAATGACTCTCTGACCCCAGAGATAGAGGCTGACCGCAGTGGCGG
  CAGAACAGATGCTGAAAGGACAATACAAGATGGAAGACTGTATTTGAAAACTACTGTCATGTACTGA
  
  Clone variation with respect to NM_080760.5
  596:t=>c 1113:t=>g 1114:t=>a 1115:t=>a 1118:t=>c 1121:t=>g 1123:t=>g 1125:a=>c 1127:t=>a 1130:t=>a 1131:t=>g 1134:a=>c 1138:a=>c 1139:g=>t
• Restriction Sites: Please inquire
• ACCN: NM_080760
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this clone has been fully sequenced and found one SNP within the protein associated with this reference, NM_080760.3.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080760.3, NP_542938.1
• RefSeq Size: 4796 bp
• RefSeq ORF: 1677 bp
• Locus ID: 1602
• Cytogenetics: 13q21.33
• Domains: Ski_Sno
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a chromatin-associated protein that associates with other DNA-binding transcription factors to regulate gene expression and cell fate determination during development. The protein contains a Ski domain that is highly conserved from Drosophila to human. Expression of this gene is lost in some forms of metastatic cancer, and is correlated with poor prognosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (2) lacks three consecutive coding exons but maintains the same reading frame, compared to variant 1. The resulting isoform (b) is shorter than isoform a. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence because a full-length transcript was not available for this variant. The genomic coordinates used for the transcript record were based on RT-PCR data reported in PMID:11543628.