SP140 (NM_007237) Human Tagged ORF Clone

CAT#: RG222828

SP140 (GFP-tagged) - Human SP140 nuclear body protein (SP140), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SP140
• Synonyms: LYSP100; LYSP100-A; LYSP100-B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222828 representing NM_007237
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGCAGGGCCAGCAGGGGCAGATGGCAAGTGGAGACAGCAATCTCAACTTCAGGATGGTCGCAG
  AGATCCAGAACGTAGAGGGTCAGAACCTGCAGGAGCAGGTTTGCCCTGAGCCCATTTTCAGGTTCTTCAG
  AGAAAACAAGGTGGAGATTGCAAGTGCAATAACAAGGCCATTTCCTTTCCTTATGGGCCTCCGAGACCGC
  TCCTTCATCTCCGAGCAGATGTATGAACATTTTCAAGAAGCTTTTAGAAACCTGGTCCCAGTGACAAGAG
  TGATGTATTGTGTACTCAGTGAACTGGAGAAGACATTTGGCTGGTCACATCTGGAAGCATTGTTCAGCAG
  GATTAACCTGATGGCCTATCCTGATTTAAACGAGATTTACAGAAGCTTCCAGAATGTATGCTATGAACAC
  TCACCTCTCCAAATGAATAATGTAAACGATTTAGAAGATAGACCCAGATTACTACCATATGGTAAACAAG
  AGAACAGCAATGCCTGTCATGAAATGGATGATATAGCAGTGCCTCAGGAAGCCTTGAGCTCCTCGCCAAG
  GTGTGAGCCAGGTTTCTCTTCAGAGTCTTGTGAGCAGTTAGCTCTCCCAAAGGCTGGTGGAGGAGATGCT
  GAAGATGCACCCAGCCTACTACCAGGTGGGGGAGTGTCCTGTAAACTTGCTATACAAATAGATGAAGGAG
  AATCAGAAGAAATGCCCAAGTTACTGCCTTATGATACAGAAGTTCTAGAAAGCAACGGGATGATAGATGC
  GGCAAGGACATACAGCACAGCACCAGGGGAGAAACAGGGAGAGGAGGAAGGCAGGAACAGTCCCAGAAAA
  AGAAACCAAGACAAGGAGAAGTACCAAGAGAGTCCAGAGGGAAGAGACAAAGAGACCTTTGATCTAAAAA
  CTCCCCAAGTCACTAATGAAGGAGAACCAGAGAAGGGGCTCTGTCTACTACCAGGTGAAGGAGAAGAGGG
  CAGTGATGACTGTTCAGAAATGTGTGATGGAGAAGAGCCCCAGGAAGCCTCTAGCTCCCTAGCAAGATGT
  GGGTCAGTTTCTTGTTTCTCTACAGAGACCTTTGATCTAAAGACTCCCCAAGTCACTAATGAAGGAGAAC
  CAGAGAAGGAGCTCAGTCTACTACCAGGTGAAGGAGAAGAGGGCAGTGATGACTGTTCGGAAATGTGTGA
  TGGAGAAGAGCGCCAGGAAGCCTCTAGCTCCCTAGCAAGACGTGGGTCAGTGTCTAGTGAACTAGAAAAT
  CACCCAATGAATGAAGAAGGAGAATCAGAAGAGCTTGCTTCTAGCCTGCTATATGATAATGTACCAGGAG
  CGGAGCAATCAGCATATGAAAATGAGAAGTGTTCCTGTGTCATGTGTTTCTCAGAAGAGGTGCCAGGAAG
  CCCAGAAGCAAGGACGGAAAGTGATCAAGCGTGTGGCACAATGGATACTGTGGATATTGCAAACAACTCC
  ACTTTGGGAAAACCCAAGAGGAAAAGAAGAAAAAAGAGGGGGCATGGCTGGAGCAGAATGAGAATGAGAA
  GGCAGAAAAACAGCCAACAAAATGATAATAGCAAAGCCGACGGCCAGGTGGTCTCCAGTGAAAAGAAGGC
  GAACGTGAATCTGAAAGACCTTTCCAAGATTAGGGGGAGAAAGAGAGGCAAACCTGGAACCCGCTTCACT
  CAGAGTGACAGAGCTGCACAGAAAAGAGTCCGATCAAGAGCTTCAAGAAAGCACAAAGATGAAACTGTGG
  ATTTTAAGGCTCCTTTGCTTCCAGTGACCTGTGGTGGGGTGAAGGGAATTTTACATAAGAAGAAATTGCA
  GCAAGGAATCTTGGTGAAGTGTATACAGACTGAGGATGGAAAATGGTTCACCCCCACGGAATTTGAAATC
  AAAGGAGGCCATGCAAGATCAAAGAACTGGAGGCTGAGTGTGCGCTGTGGCGGGTGGCCCCTACGATGGC
  TGATGGAGAATGGATTTCTGCCTGATCCTCCAAGAATACGTTACAGGAAAAAAAAGAGAATACTGAAGTC
  TCAAAACAATAGCTCAGTTGACCCTTGTATGAGAAACCTGGATGAGTGTGAGGTGTGCCGGGACGGAGGG
  GAGCTGTTCTGTTGCGACACTTGTTCAAGAGTCTTCCATGAGGACTGTCACATCCCGCCTGTGGAAGCTG
  AGAGGACCCCGTGGAATTGCATCTTCTGCAGGATGAAGGAGTCTCCGGGAAGCCAACAGTGTTGTCAGGA
  ATCTGAGGTCCTGGAGAGGCAGATGTGTCCTGAGGAACAGTTGAAATGTGAGTTCCTCCTCTTGAAAGTC
  TATTGCTGTTCTGAGAGCTCCTTTTTTGCCAAGATTCCATACTATTATTATATTAGAGAGGCGTGTCAAG
  GCCTGAAGGAGCCCATGTGGTTGGATAAAATCAAGAAAAGGCTGAATGAGCACGGTTACCCCCAAGTGGA
  GGGGTTTGTACAAGACATGCGCCTCATCTTCCAGAACCACAGGGCCTCTTACAAGTACAAGGATTTTGGC
  CAAATGGGATTTAGACTGGAGGCTGAGTTTGAGAAGAATTTCAAGGAAGTGTTTGCTATTCAGGAAACAA
  ATGGGAACAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222828 representing NM_007237
  Red=Cloning site Green=Tags(s)
  
  MAQQGQQGQMASGDSNLNFRMVAEIQNVEGQNLQEQVCPEPIFRFFRENKVEIASAITRPFPFLMGLRDR
  SFISEQMYEHFQEAFRNLVPVTRVMYCVLSELEKTFGWSHLEALFSRINLMAYPDLNEIYRSFQNVCYEH
  SPLQMNNVNDLEDRPRLLPYGKQENSNACHEMDDIAVPQEALSSSPRCEPGFSSESCEQLALPKAGGGDA
  EDAPSLLPGGGVSCKLAIQIDEGESEEMPKLLPYDTEVLESNGMIDAARTYSTAPGEKQGEEEGRNSPRK
  RNQDKEKYQESPEGRDKETFDLKTPQVTNEGEPEKGLCLLPGEGEEGSDDCSEMCDGEEPQEASSSLARC
  GSVSCFSTETFDLKTPQVTNEGEPEKELSLLPGEGEEGSDDCSEMCDGEERQEASSSLARRGSVSSELEN
  HPMNEEGESEELASSLLYDNVPGAEQSAYENEKCSCVMCFSEEVPGSPEARTESDQACGTMDTVDIANNS
  TLGKPKRKRRKKRGHGWSRMRMRRQKNSQQNDNSKADGQVVSSEKKANVNLKDLSKIRGRKRGKPGTRFT
  QSDRAAQKRVRSRASRKHKDETVDFKAPLLPVTCGGVKGILHKKKLQQGILVKCIQTEDGKWFTPTEFEI
  KGGHARSKNWRLSVRCGGWPLRWLMENGFLPDPPRIRYRKKKRILKSQNNSSVDPCMRNLDECEVCRDGG
  ELFCCDTCSRVFHEDCHIPPVEAERTPWNCIFCRMKESPGSQQCCQESEVLERQMCPEEQLKCEFLLLKV
  YCCSESSFFAKIPYYYYIREACQGLKEPMWLDKIKKRLNEHGYPQVEGFVQDMRLIFQNHRASYKYKDFG
  QMGFRLEAEFEKNFKEVFAIQETNGNN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007237
• ORF Size: 2601 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007237.3, NP_009168.3
• RefSeq Size: 3269
• RefSeq ORF: 2604
• Locus ID: 11262
• Domains: SAND, BROMO, PHD, Sp100
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a member of the SP100 family of proteins, which are share common domains including an N-terminal homogeneously staining region domain followed by a SP100/autoimmune regulator/NucP41/P75/deformed epidermal autoregulatory factor domain, a plant homeobox zinc finger, and a bromodomain. The encoded protein is interferon-inducible and is expressed at high levels in the nuclei of leukocytes. Variants of this gene have been associated with multiple sclerosis, Crohn's disease, and chronic lymphocytic leukemia. Alternative splicing results in multiple variants. [provided by RefSeq, Aug 2016]